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{
            
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  "author": "Laurent, Julien",
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  "author": "Laurent, Julien",
            
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  "author_email": "",
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  "author_email": "",
            
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  "citation": [],
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  "citation": [],
            
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  "creator_user_id": "17755db4-395a-4b3b-ac09-e8e3484ca700",
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  "creator_user_id": "17755db4-395a-4b3b-ac09-e8e3484ca700",
            
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  "doi": "10.1594/PANGAEA.837087",
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  "doi": "10.1594/PANGAEA.837087",
            
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  "doi_date_published": "2013",
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  "doi_date_published": "2013",
            
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  "doi_publisher": "",
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  "doi_publisher": "",
            
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  "doi_status": "True",
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  "doi_status": "True",
            
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  "extra_authors": [
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  "extra_authors": [
            
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    {
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    {
            
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      "extra_author": "Venn, Alexander A",
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      "extra_author": "Venn, Alexander A",
            
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      "familyName": "Venn",
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      "familyName": "Venn",
            
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      "givenName": "Alexander A",
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      "givenName": "Alexander A",
            
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      "orcid": "0000-0003-0544-0884"
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      "orcid": "0000-0003-0544-0884"
            
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    },
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    },
            
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    {
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    {
            
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      "extra_author": "Tambutt\u00e9, Eric",
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      "extra_author": "Tambutt\u00e9, Eric",
            
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      "familyName": "Tambutt\u00e9",
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      "familyName": "Tambutt\u00e9",
            
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      "givenName": "Eric",
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      "givenName": "Eric",
            
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      "orcid": "0000-0002-1419-3785"
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      "orcid": "0000-0002-1419-3785"
            
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    },
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    },
            
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    {
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    {
            
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      "extra_author": "Ganot, Philippe",
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      "extra_author": "Ganot, Philippe",
            
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      "familyName": "Ganot",
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      "familyName": "Ganot",
            
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      "givenName": "Philippe",
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      "givenName": "Philippe",
            
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      "orcid": ""
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      "orcid": ""
            
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    },
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    },
            
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    {
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    {
            
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      "extra_author": "Allemand, Denis",
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      "extra_author": "Allemand, Denis",
            
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      "familyName": "Allemand",
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      "familyName": "Allemand",
            
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      "givenName": "Denis",
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      "givenName": "Denis",
            
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      "orcid": ""
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      "orcid": ""
            
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    },
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    },
            
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    {
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    {
            
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      "extra_author": "Tambutt\u00e9, Sylvie",
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      "extra_author": "Tambutt\u00e9, Sylvie",
            
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      "familyName": "Tambutt\u00e9",
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      "familyName": "Tambutt\u00e9",
            
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      "givenName": "Sylvie",
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      "givenName": "Sylvie",
            
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      "orcid": "0000-0002-0505-6375"
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      "orcid": "0000-0002-0505-6375"
            
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    }
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    }
            
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  ],
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  ],
            
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  "familyName": "Laurent",
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  "familyName": "Laurent",
            
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  "givenName": "Julien",
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  "givenName": "Julien",
            
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  "groups": [],
            
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  "license_id": "CC-BY-3.0",
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  "license_id": "CC-BY-3.0",
            
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  "license_title": "CC-BY-3.0",
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  "license_title": "CC-BY-3.0",
            
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  "metadata_created": "2024-11-29T11:45:15.386740",
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  "metadata_created": "2024-11-29T11:45:15.386740",
            
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  "metadata_modified": "2024-11-29T11:45:15.386746",
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  "metadata_modified": "2024-11-30T14:30:05.270087",
            
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  "name": "png-doi-10-1594-pangaea-837087",
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  "name": "png-doi-10-1594-pangaea-837087",
            
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  "notes": "The regulation of intracellular pH (pHi) is a fundamental 
52
  "notes": "The regulation of intracellular pH (pHi) is a fundamental 
            
53
aspect of cell physiology that has received little attention in 
53
aspect of cell physiology that has received little attention in 
            
54
studies of the phylum Cnidaria, which includes ecologically important 
54
studies of the phylum Cnidaria, which includes ecologically important 
            
55
sea anemones and reef-building corals. Like all organisms, cnidarians 
55
sea anemones and reef-building corals. Like all organisms, cnidarians 
            
56
must maintain pH homeostasis to counterbalance reductions in pHi, 
56
must maintain pH homeostasis to counterbalance reductions in pHi, 
            
57
which can arise because of changes in either intrinsic or extrinsic 
57
which can arise because of changes in either intrinsic or extrinsic 
            
58
parameters. Corals and sea anemones face natural daily changes in 
58
parameters. Corals and sea anemones face natural daily changes in 
            
59
internal fluids, where the extracellular pH can range from 8.9 during 
59
internal fluids, where the extracellular pH can range from 8.9 during 
            
60
the day to 7.4 at night. Furthermore, cnidarians are likely to 
60
the day to 7.4 at night. Furthermore, cnidarians are likely to 
            
61
experience future CO2-driven declines in seawater pH, a process known 
61
experience future CO2-driven declines in seawater pH, a process known 
            
62
as ocean acidification. Here, we carried out the first mechanistic 
62
as ocean acidification. Here, we carried out the first mechanistic 
            
63
investigation to determine how cnidarian pHi regulation responds to 
63
investigation to determine how cnidarian pHi regulation responds to 
            
64
decreases in extracellular and intracellular pH. Using the anemone 
64
decreases in extracellular and intracellular pH. Using the anemone 
            
65
Anemonia viridis, we employed confocal live cell imaging and a 
65
Anemonia viridis, we employed confocal live cell imaging and a 
            
66
pH-sensitive dye to track the dynamics of pHi after intracellular 
66
pH-sensitive dye to track the dynamics of pHi after intracellular 
            
67
acidosis induced by acute exposure to decreases in seawater pH and 
67
acidosis induced by acute exposure to decreases in seawater pH and 
            
68
NH4Cl prepulses. The investigation was conducted on cells that 
68
NH4Cl prepulses. The investigation was conducted on cells that 
            
69
contained intracellular symbiotic algae (Symbiodinium sp.) and on 
69
contained intracellular symbiotic algae (Symbiodinium sp.) and on 
            
70
symbiont-free endoderm cells. Experiments using inhibitors and Na-free 
70
symbiont-free endoderm cells. Experiments using inhibitors and Na-free 
            
71
seawater indicate a potential role of Na/H plasma membrane exchangers 
71
seawater indicate a potential role of Na/H plasma membrane exchangers 
            
72
(NHEs) in mediating pHi recovery following intracellular acidosis in 
72
(NHEs) in mediating pHi recovery following intracellular acidosis in 
            
73
both cell types. We also measured the buffering capacity of cells, and 
73
both cell types. We also measured the buffering capacity of cells, and 
            
74
obtained values between 20.8 and 43.8 mM per pH unit, which are 
74
obtained values between 20.8 and 43.8 mM per pH unit, which are 
            
75
comparable to those in other invertebrates. Our findings provide the 
75
comparable to those in other invertebrates. Our findings provide the 
            
76
first steps towards a better understanding of acid-base regulation in 
76
first steps towards a better understanding of acid-base regulation in 
            
77
these basal metazoans, for which information on cell physiology is 
77
these basal metazoans, for which information on cell physiology is 
            
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extremely limited.",
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extremely limited.",
            
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    "approval_status": "approved",
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    "approval_status": "approved",
            
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    "created": "2024-11-30T14:19:14.458899",
            
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    "description": "PANGAEA (Data Publisher for Earth & Environmental 
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    "description": "PANGAEA (Data Publisher for Earth & Environmental 
            
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Science): The information system PANGAEA is operated as an Open Access 
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Science): The information system PANGAEA is operated as an Open Access 
            
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library aimed at archiving, publishing and distributing georeferenced 
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library aimed at archiving, publishing and distributing georeferenced 
            
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data from earth system research. PANGAEA guarantees long-term 
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data from earth system research. PANGAEA guarantees long-term 
            
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availability (greater than 10 years) of its content. PANGAEA is open 
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availability (greater than 10 years) of its content. PANGAEA is open 
            
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to any project, institution, or individual scientist to use or to 
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to any project, institution, or individual scientist to use or to 
            
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archive and publish data. PANGAEA focuses on georeferenced 
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archive and publish data. PANGAEA focuses on georeferenced 
            
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observational data, experimental data, and models/simulations. 
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observational data, experimental data, and models/simulations. 
            
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Citability, comprehensive metadata descriptions, interoperability of 
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Citability, comprehensive metadata descriptions, interoperability of 
            
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data and metadata, a high degree of structural and semantic 
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data and metadata, a high degree of structural and semantic 
            
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harmonization of the data inventory as well as the commitment of the 
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harmonization of the data inventory as well as the commitment of the 
            
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hosting institutions ensures FAIRness of archived data.",
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hosting institutions ensures FAIRness of archived data.",
            
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    "name": "pangaea_biosphere",
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    "name": "pangaea_biologicalclassification",
            
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    "state": "active",
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    "state": "active",
            
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    "title": "PANGAEA (Biosphere)",
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    "title": "PANGAEA (Biological Classification)",
            
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    "type": "organization"
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    "type": "organization"
            
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  },
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  },
            
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  "owner_org": "3226ef9c-20d1-43fd-ba8f-fa35c8c9fb5d",
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  "publication_year": "2013",
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  "publication_year": "2013",
            
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  "related_identifiers": [
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  "related_identifiers": [
            
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    {
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    {
            
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      "authors": "Laurent Julien,Venn Alexander A,Tambutt\u00e9 
110
      "authors": "Laurent Julien,Venn Alexander A,Tambutt\u00e9 
            
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Eric,Ganot Philippe,Allemand Denis,Tambutt\u00e9 Sylvie",
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Eric,Ganot Philippe,Allemand Denis,Tambutt\u00e9 Sylvie",
            
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      "email_authors": 
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      "email_authors": 
            
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,allemand@centrescientifique.mc,stambutt\u00e9@centrescientifique.mc",
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,allemand@centrescientifique.mc,stambutt\u00e9@centrescientifique.mc",
            
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      "identifier": "https://doi.org/10.1111/febs.12614",
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      "identifier": "https://doi.org/10.1111/febs.12614",
            
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      "identifier_type": "DOI",
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      "identifier_type": "DOI",
            
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      "orcid_authors": 
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      "orcid_authors": 
            
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",0000-0003-0544-0884,0000-0002-1419-3785,,,0000-0002-0505-6375",
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",0000-0003-0544-0884,0000-0002-1419-3785,,,0000-0002-0505-6375",
            
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      "relation_type": "IsSupplementTo",
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      "relation_type": "IsSupplementTo",
            
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      "source": "FEBS Journal",
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      "source": "FEBS Journal",
            
120
      "title": "Regulation of intracellular pH in cnidarians: response 
120
      "title": "Regulation of intracellular pH in cnidarians: response 
            
121
to acidosis in Anemonia viridis",
121
to acidosis in Anemonia viridis",
            
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      "year": "2014"
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      "year": "2014"
            
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    },
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    },
            
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    {
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    {
            
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      "authors": "Lavigne H\u00e9lo\u00efse,Epitalon 
125
      "authors": "Lavigne H\u00e9lo\u00efse,Epitalon 
            
126
Jean-Marie,Gattuso Jean-Pierre",
126
Jean-Marie,Gattuso Jean-Pierre",
            
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      "email_authors": 
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      "email_authors": 
            
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"hlavigne@naturalsciences.be,,jean-pierre.gattuso@imev-mer.fr",
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"hlavigne@naturalsciences.be,,jean-pierre.gattuso@imev-mer.fr",
            
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      "identifier": "https://cran.r-project.org/package=seacarb",
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      "identifier": "https://cran.r-project.org/package=seacarb",
            
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      "identifier_type": "DOI",
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      "identifier_type": "DOI",
            
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      "orcid_authors": ",,0000-0002-4533-4114",
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      "orcid_authors": ",,0000-0002-4533-4114",
            
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      "relation_type": "References",
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      "relation_type": "References",
            
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      "source": "",
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      "source": "",
            
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      "title": "seacarb: seawater carbonate chemistry with R. R 
134
      "title": "seacarb: seawater carbonate chemistry with R. R 
            
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package version 3.0",
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package version 3.0",
            
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      "year": "2014"
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      "year": "2014"
            
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    }
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    }
            
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  ],
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  "repository_name": "PANGAEA (Data Publisher for Earth & 
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  "repository_name": "PANGAEA (Data Publisher for Earth & 
            
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Environmental Science)",
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Environmental Science)",
            
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  "resource_type": "text/tab-separated-values - filename: 
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  "resource_type": "text/tab-separated-values - filename: 
            
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Laurent_2014",
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Laurent_2014",
            
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  "resources": [],
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  "resources": [],
            
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  "source_metadata_created": "2013",
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  "source_metadata_created": "2013",
            
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  "source_metadata_modified": "",
            
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  "state": "active",
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  "state": "active",
            
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    {
            
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      "subject_area_additional": "",
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      "subject_area_additional": "",
            
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      "subject_area_name": "BiologicalClassification"
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      "subject_area_name": "BiologicalClassification"
            
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    },
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    },
            
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    {
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    {
            
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      "subject_area_additional": "",
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      "subject_area_additional": "",
            
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      "subject_area_name": "Biosphere"
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      "subject_area_name": "Biosphere"
            
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    },
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    },
            
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    {
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    {
            
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      "subject_area_additional": "",
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      "subject_area_additional": "",
            
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      "subject_area_name": "Chemistry"
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      "subject_area_name": "Chemistry"
            
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    }
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    }
            
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  ],
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  ],
            
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  "tags": [
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  "tags": [
            
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    {
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    {
            
n
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      "display_name": "Acid-base regulation",
n
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      "display_name": "Acid",
            
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      "id": "cacede69-85cf-4701-9659-7886f241718e",
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      "id": "47822529-283b-45e4-b112-eb25b4c31993",
            
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      "name": "Acid-base regulation",
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      "name": "Acid",
            
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      "state": "active",
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      "state": "active",
            
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      "vocabulary_id": null
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      "vocabulary_id": null
            
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    },
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    },
            
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    {
171
    {
            
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      "display_name": "Anemonia viridis",
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      "display_name": "Anemonia viridis",
            
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      "id": "a8b9d3a1-8b2e-4686-8eb1-1e581f066b82",
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      "id": "a8b9d3a1-8b2e-4686-8eb1-1e581f066b82",
            
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      "name": "Anemonia viridis",
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      "name": "Anemonia viridis",
            
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      "state": "active",
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      "state": "active",
            
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      "vocabulary_id": null
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      "vocabulary_id": null
            
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    },
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    },
            
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    {
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    {
            
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      "display_name": "Animalia",
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      "display_name": "Animalia",
            
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      "id": "e645e194-a2f7-40f1-ae7c-c1496be6f6bf",
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      "id": "e645e194-a2f7-40f1-ae7c-c1496be6f6bf",
            
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      "name": "Animalia",
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      "name": "Animalia",
            
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      "state": "active",
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      "state": "active",
            
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      "vocabulary_id": null
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      "vocabulary_id": null
            
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    },
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    },
            
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    {
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    {
            
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      "display_name": "Aquaria 20 L",
            
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      "id": "62c412c1-2294-406a-ad79-183330400def",
            
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      "name": "Aquaria 20 L",
            
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      "state": "active",
            
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      "vocabulary_id": null
            
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    },
            
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    {
            
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      "display_name": "Benthic animals",
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      "display_name": "Benthic animals",
            
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      "id": "ba711ac9-d2d3-4cff-8023-8b85163344bb",
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      "id": "ba711ac9-d2d3-4cff-8023-8b85163344bb",
            
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      "name": "Benthic animals",
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      "name": "Benthic animals",
            
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      "state": "active",
196
      "state": "active",
            
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      "vocabulary_id": null
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      "vocabulary_id": null
            
191
    },
198
    },
            
192
    {
199
    {
            
193
      "display_name": "Benthos",
200
      "display_name": "Benthos",
            
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      "id": "55bc70f2-ff73-4d9a-89f6-16358d0d9b87",
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      "id": "55bc70f2-ff73-4d9a-89f6-16358d0d9b87",
            
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      "name": "Benthos",
202
      "name": "Benthos",
            
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      "state": "active",
203
      "state": "active",
            
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      "vocabulary_id": null
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      "vocabulary_id": null
            
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    },
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    },
            
199
    {
206
    {
            
n
200
      "display_name": "Bottles or small containers-Aquaria 20 L",
n
207
      "display_name": "Bottles or small containers",
            
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      "id": "be154617-73a3-4ed4-b54f-69e22323c12a",
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      "id": "ba227243-af91-4efc-8086-790112e90e09",
            
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      "name": "Bottles or small containers-Aquaria 20 L",
209
      "name": "Bottles or small containers",
            
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      "state": "active",
210
      "state": "active",
            
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      "vocabulary_id": null
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      "vocabulary_id": null
            
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    },
212
    },
            
206
    {
213
    {
            
207
      "display_name": "Cnidaria",
214
      "display_name": "Cnidaria",
            
208
      "id": "5c3a6df6-61df-4230-b9e3-7768cbada71d",
215
      "id": "5c3a6df6-61df-4230-b9e3-7768cbada71d",
            
209
      "name": "Cnidaria",
216
      "name": "Cnidaria",
            
210
      "state": "active",
217
      "state": "active",
            
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      "vocabulary_id": null
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      "vocabulary_id": null
            
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    },
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    },
            
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    {
220
    {
            
214
      "display_name": "Coast and continental shelf",
221
      "display_name": "Coast and continental shelf",
            
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      "id": "58eb1929-34b5-4fd9-ac44-824f0e51de40",
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      "id": "58eb1929-34b5-4fd9-ac44-824f0e51de40",
            
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      "name": "Coast and continental shelf",
223
      "name": "Coast and continental shelf",
            
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      "state": "active",
224
      "state": "active",
            
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      "vocabulary_id": null
225
      "vocabulary_id": null
            
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    },
226
    },
            
220
    {
227
    {
            
221
      "display_name": "Laboratory experiment",
228
      "display_name": "Laboratory experiment",
            
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      "id": "c6c00754-c769-4932-a517-bff2c9512884",
229
      "id": "c6c00754-c769-4932-a517-bff2c9512884",
            
223
      "name": "Laboratory experiment",
230
      "name": "Laboratory experiment",
            
224
      "state": "active",
231
      "state": "active",
            
225
      "vocabulary_id": null
232
      "vocabulary_id": null
            
226
    },
233
    },
            
227
    {
234
    {
            
228
      "display_name": "Mediterranean Sea",
235
      "display_name": "Mediterranean Sea",
            
229
      "id": "3aca1dd8-7465-4a62-a7b5-d877160a2ef6",
236
      "id": "3aca1dd8-7465-4a62-a7b5-d877160a2ef6",
            
230
      "name": "Mediterranean Sea",
237
      "name": "Mediterranean Sea",
            
231
      "state": "active",
238
      "state": "active",
            
232
      "vocabulary_id": null
239
      "vocabulary_id": null
            
233
    },
240
    },
            
234
    {
241
    {
            
235
      "display_name": "Respiration",
242
      "display_name": "Respiration",
            
236
      "id": "494c54c9-c9ac-44d3-88fa-8e717d8113e0",
243
      "id": "494c54c9-c9ac-44d3-88fa-8e717d8113e0",
            
237
      "name": "Respiration",
244
      "name": "Respiration",
            
238
      "state": "active",
245
      "state": "active",
            
239
      "vocabulary_id": null
246
      "vocabulary_id": null
            
240
    },
247
    },
            
241
    {
248
    {
            
242
      "display_name": "Single species",
249
      "display_name": "Single species",
            
243
      "id": "88de69be-3bdd-4cf8-b6a1-386d72c5888d",
250
      "id": "88de69be-3bdd-4cf8-b6a1-386d72c5888d",
            
244
      "name": "Single species",
251
      "name": "Single species",
            
245
      "state": "active",
252
      "state": "active",
            
246
      "vocabulary_id": null
253
      "vocabulary_id": null
            
247
    },
254
    },
            
248
    {
255
    {
            
249
      "display_name": "Temperate",
256
      "display_name": "Temperate",
            
250
      "id": "31aaeb0c-a4a5-4a8e-9c90-be1eff0cac91",
257
      "id": "31aaeb0c-a4a5-4a8e-9c90-be1eff0cac91",
            
251
      "name": "Temperate",
258
      "name": "Temperate",
            
t
t
259
      "state": "active",
            
260
      "vocabulary_id": null
            
261
    },
            
262
    {
            
263
      "display_name": "base regulation",
            
264
      "id": "7e6acb21-37f2-4d0b-89e9-0ad04a1db8aa",
            
265
      "name": "base regulation",
            
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      "state": "active",
266
      "state": "active",
            
253
      "vocabulary_id": null
267
      "vocabulary_id": null
            
254
    }
268
    }
            
255
  ],
269
  ],
            
256
  "title": "Regulation of intracellular pH in cnidarians: response to 
270
  "title": "Regulation of intracellular pH in cnidarians: response to 
            
257
acidosis in Anemonia viridis",
271
acidosis in Anemonia viridis",
            
258
  "type": "vdataset",
272
  "type": "vdataset",
            
259
  "url": "https://doi.org/10.1594/PANGAEA.837087"
273
  "url": "https://doi.org/10.1594/PANGAEA.837087"
            
260
}
274
}