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Molecular and biochemical analyses of wounding in mesocarp of peach (Prunus persica L. Batsch) ripe fruits

The physiological and molecular responses of ripe fruit to wounding were evaluated in two peach (Prunus persica) varieties ('Glohaven', GH, melting and 'BigTop', BT, slow melting nectarine) by comparing mesocarp samples from wedges (as in minimal processing) and whole fruit as the control. Slight differences between the two varieties were detected in terms of ethylene production, whereas total phenol and flavonoid concentrations, and PPO and POD enzyme activities showed a general increase in wounded GH but not in BT. This was associated with the better appearance of the BT wedges at the end of the experimental period (72 h). Microarray (genome-wide mPEACH3.0) analysis revealed that a total number of 2218 genes were differentially expressed (p 1 or <-1) in GH 24 h after wounding compared to the control. This number was much lower (1208) in BT. According to the enrichment analysis, cell wall, plasma membrane, response to stress, secondary metabolic processes, oxygen binding were the GO categories over-represented among the GH up-regulated genes, whereas plasma membrane and response to endogenous stimulus were the categories over-represented among the down-regulated genes. Only 32 genes showed a common expression trend in the two varieties 24 h after wounding, whereas a total of 512 genes (with highly represented transcription factors), displayed opposite behavior. Quantitative RT-PCR analysis confirmed the microarray data for 18 out of a total of 20 genes selected. Specific WRKY, AP2/ERF and HSP20 genes were markedly up-regulated in wounded GH, indicating the activation of regulatory and signaling mechanisms probably related to different hormone categories. Compared to BT, the expression of specific genes involved in phenylpropanoid and triterpenoid biosynthetic pathways showed a more pronounced induction in GH, highlighting the difference between the two peach varieties in terms of molecular responses to wounding in the mesocarp tissue.

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Tosetti Roberta, Tadiello Alice, Zaffalon Valerio, Giorgi Federico M, Guidi Lucia, Trainotti Livio, Tonutti Pietro (2014). Dataset: Molecular and biochemical analyses of wounding in mesocarp of peach (Prunus persica L. Batsch) ripe fruits. https://doi.org/10.1594/PANGAEA.819776

DOI retrieved: 2014

Additional Info

Field Value
Imported on January 12, 2023
Last update August 4, 2023
License CC-BY-3.0
Source https://doi.org/10.1594/PANGAEA.819776
Author Tosetti Roberta
More Authors
Tadiello Alice
Zaffalon Valerio
Giorgi Federico M
Guidi Lucia
Trainotti Livio
Tonutti Pietro
Source Creation 2014
Publication Year 2014
Resource Type text/tab-separated-values - filename: Tosetti_2014
Subject Areas
Name: Agriculture

Name: Ecology

Name: HumanDimensions

Related Identifiers
Title: Molecular and biochemical responses to wounding in mesocarp of ripe peach (Prunus persica L. Batsch) fruit
Identifier: https://doi.org/10.1016/j.postharvbio.2013.12.001
Type: DOI
Relation: IsSupplementTo
Year: 2014
Source: Postharvest Biology and Technology
Authors: Tosetti Roberta , Tardelli Francesca , Tadiello Alice , Zaffalon Valerio , Giorgi Federico M , Guidi Lucia , Trainotti Livio , Bonghi Claudio , Tonutti Pietro .