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{
            
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  "author": "Endo, H",
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  "author": "Endo, H",
            
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  "author_email": "",
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  "author_email": "",
            
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  "citation": [],
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  "citation": [],
            
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  "creator_user_id": "17755db4-395a-4b3b-ac09-e8e3484ca700",
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  "creator_user_id": "17755db4-395a-4b3b-ac09-e8e3484ca700",
            
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  "doi": "10.1594/PANGAEA.820333",
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  "doi": "10.1594/PANGAEA.820333",
            
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  "doi_date_published": "2013",
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  "doi_date_published": "2013",
            
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  "doi_publisher": "",
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  "doi_publisher": "",
            
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  "doi_status": "True",
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  "doi_status": "True",
            
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  "extra_authors": [
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  "extra_authors": [
            
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    {
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    {
            
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      "extra_author": "Yoshimura, T",
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      "extra_author": "Yoshimura, T",
            
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      "familyName": "Yoshimura",
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      "familyName": "Yoshimura",
            
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      "givenName": "T",
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      "givenName": "T",
            
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      "orcid": "0000-0001-5700-2590"
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      "orcid": "0000-0001-5700-2590"
            
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    },
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    },
            
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    {
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    {
            
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      "extra_author": "Kataoka, Takafumi",
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      "extra_author": "Kataoka, Takafumi",
            
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      "familyName": "Kataoka",
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      "familyName": "Kataoka",
            
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      "givenName": "Takafumi",
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      "givenName": "Takafumi",
            
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      "orcid": ""
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      "orcid": ""
            
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    },
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    },
            
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    {
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    {
            
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      "extra_author": "Suzuki, Koji",
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      "extra_author": "Suzuki, Koji",
            
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      "familyName": "Suzuki",
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      "familyName": "Suzuki",
            
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      "givenName": "Koji",
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      "givenName": "Koji",
            
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      "orcid": "0000-0001-5354-1044"
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      "orcid": "0000-0001-5354-1044"
            
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    }
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    }
            
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  ],
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  ],
            
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  "familyName": "Endo",
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  "familyName": "Endo",
            
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  "givenName": "H",
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  "givenName": "H",
            
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  "license_id": "CC-BY-3.0",
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  "license_id": "CC-BY-3.0",
            
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  "license_title": "CC-BY-3.0",
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  "license_title": "CC-BY-3.0",
            
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  "metadata_created": "2024-11-29T11:34:39.608622",
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  "metadata_created": "2024-11-29T11:34:39.608622",
            
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  "metadata_modified": "2024-11-29T11:34:39.608629",
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  "metadata_modified": "2024-11-30T10:40:59.606048",
            
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  "name": "png-doi-10-1594-pangaea-820333",
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  "name": "png-doi-10-1594-pangaea-820333",
            
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  "notes": "On-deck CO2-Fe-manipulated incubation experiments were 
40
  "notes": "On-deck CO2-Fe-manipulated incubation experiments were 
            
41
conducted using surface seawater collected from the Western Subarctic 
41
conducted using surface seawater collected from the Western Subarctic 
            
42
Gyre of the NW Pacific in the summer of 2008 to elucidate the impacts 
42
Gyre of the NW Pacific in the summer of 2008 to elucidate the impacts 
            
43
of ocean acidification and Fe enrichment on the abundance and 
43
of ocean acidification and Fe enrichment on the abundance and 
            
44
community composition of phytoplankton and eubacteria in the study 
44
community composition of phytoplankton and eubacteria in the study 
            
45
area. During the incubation, excluding the initial period, the mean 
45
area. During the incubation, excluding the initial period, the mean 
            
46
partial pressures of CO2 in non-Fe-added bottles were 230, 419, 843, 
46
partial pressures of CO2 in non-Fe-added bottles were 230, 419, 843, 
            
47
and 1124 \u00b5atm, whereas those in Fe-added treatments were 152, 
47
and 1124 \u00b5atm, whereas those in Fe-added treatments were 152, 
            
48
394, 791, and 1008 \u00b5atm. Changes in the abundance and community 
48
394, 791, and 1008 \u00b5atm. Changes in the abundance and community 
            
49
composition of phytoplankton were estimated using HPLC pigment 
49
composition of phytoplankton were estimated using HPLC pigment 
            
50
signatures with the program CHEMTAX and flow cytometry. A DGGE 
50
signatures with the program CHEMTAX and flow cytometry. A DGGE 
            
51
fingerprint technique targeting 16S rRNA gene fragments was also used 
51
fingerprint technique targeting 16S rRNA gene fragments was also used 
            
52
to estimate changes in eubacterial phylotypes during incubation. The 
52
to estimate changes in eubacterial phylotypes during incubation. The 
            
53
Fe addition induced diatom blooms, and subsequently stimulated the 
53
Fe addition induced diatom blooms, and subsequently stimulated the 
            
54
growth of heterotrophic bacteria such as Roseobacter, Phaeobacter, and 
54
growth of heterotrophic bacteria such as Roseobacter, Phaeobacter, and 
            
55
Alteromonas in the post-bloom phase. In both the Fe-limited and 
55
Alteromonas in the post-bloom phase. In both the Fe-limited and 
            
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Fe-replete treatments, concentrations of 19'-hexanoyloxyfucoxanthin, a 
56
Fe-replete treatments, concentrations of 19'-hexanoyloxyfucoxanthin, a 
            
57
haptophyte marker, and the cell abundance of coccolithophores 
57
haptophyte marker, and the cell abundance of coccolithophores 
            
58
decreased at higher CO2 levels (750 and 1000 ppm), whereas diatoms 
58
decreased at higher CO2 levels (750 and 1000 ppm), whereas diatoms 
            
59
exhibited little response to the changes in CO2 availability. The 
59
exhibited little response to the changes in CO2 availability. The 
            
60
abundances of Synechococcus and small eukaryotic phytoplankton (<10 
60
abundances of Synechococcus and small eukaryotic phytoplankton (<10 
            
61
\u00b5m) increased at the higher CO2 levels. DGGE band positions 
61
\u00b5m) increased at the higher CO2 levels. DGGE band positions 
            
62
revealed that Methylobacterium of Alphaproteobacteria occurred solely 
62
revealed that Methylobacterium of Alphaproteobacteria occurred solely 
            
63
at lower CO2 levels (180 and 380 ppm) during the post-bloom phase. 
63
at lower CO2 levels (180 and 380 ppm) during the post-bloom phase. 
            
64
These results suggest that increases in CO2 level could affect not 
64
These results suggest that increases in CO2 level could affect not 
            
65
only the community composition of phytoplankton but also that of 
65
only the community composition of phytoplankton but also that of 
            
66
eubacteria. As these microorganisms play critical roles in the 
66
eubacteria. As these microorganisms play critical roles in the 
            
67
biological carbon pump and microbial loop, our results indicate that 
67
biological carbon pump and microbial loop, our results indicate that 
            
68
the progression of ocean acidification can alter the biogeochemical 
68
the progression of ocean acidification can alter the biogeochemical 
            
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processes in the study area.",
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processes in the study area.",
            
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    "approval_status": "approved",
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    "approval_status": "approved",
            
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    "description": "PANGAEA (Data Publisher for Earth & Environmental 
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Science): The information system PANGAEA is operated as an Open Access 
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Science): The information system PANGAEA is operated as an Open Access 
            
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library aimed at archiving, publishing and distributing georeferenced 
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library aimed at archiving, publishing and distributing georeferenced 
            
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data from earth system research. PANGAEA guarantees long-term 
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data from earth system research. PANGAEA guarantees long-term 
            
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availability (greater than 10 years) of its content. PANGAEA is open 
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availability (greater than 10 years) of its content. PANGAEA is open 
            
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to any project, institution, or individual scientist to use or to 
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to any project, institution, or individual scientist to use or to 
            
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archive and publish data. PANGAEA focuses on georeferenced 
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archive and publish data. PANGAEA focuses on georeferenced 
            
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observational data, experimental data, and models/simulations. 
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observational data, experimental data, and models/simulations. 
            
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Citability, comprehensive metadata descriptions, interoperability of 
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Citability, comprehensive metadata descriptions, interoperability of 
            
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data and metadata, a high degree of structural and semantic 
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data and metadata, a high degree of structural and semantic 
            
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harmonization of the data inventory as well as the commitment of the 
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harmonization of the data inventory as well as the commitment of the 
            
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hosting institutions ensures FAIRness of archived data.",
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hosting institutions ensures FAIRness of archived data.",
            
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    "name": "pangaea_biosphere",
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    "name": "pangaea_ecology",
            
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    "state": "active",
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    "state": "active",
            
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    "title": "PANGAEA (Biosphere)",
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    "title": "PANGAEA (Ecology)",
            
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    "type": "organization"
            
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  "publication_year": "2013",
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  "related_identifiers": [
            
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    {
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    {
            
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      "authors": "Endo H,Yoshimura T,Kataoka Takafumi,Suzuki Koji",
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      "authors": "Endo H,Yoshimura T,Kataoka Takafumi,Suzuki Koji",
            
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      "email_authors": 
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      "email_authors": 
            
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"endo@ees.hokudai.ac.jp,ytakeshi@criepi.denken.or.jp,,",
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"endo@ees.hokudai.ac.jp,ytakeshi@criepi.denken.or.jp,,",
            
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      "identifier": "https://doi.org/10.1016/j.jembe.2012.11.003",
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      "identifier": "https://doi.org/10.1016/j.jembe.2012.11.003",
            
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      "identifier_type": "DOI",
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      "identifier_type": "DOI",
            
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      "orcid_authors": 
            
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      "relation_type": "IsSupplementTo",
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      "relation_type": "IsSupplementTo",
            
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      "source": "Journal of Experimental Marine Biology and Ecology",
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      "source": "Journal of Experimental Marine Biology and Ecology",
            
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      "title": "Effects of CO2 and iron availability on phytoplankton 
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      "title": "Effects of CO2 and iron availability on phytoplankton 
            
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and eubacterial community compositions in the northwest subarctic 
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and eubacterial community compositions in the northwest subarctic 
            
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Pacific",
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Pacific",
            
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      "year": "2013"
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      "year": "2013"
            
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    },
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    },
            
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    {
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    {
            
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      "authors": "Lavigne H\u00e9lo\u00efse,Gattuso Jean-Pierre",
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      "authors": "Lavigne H\u00e9lo\u00efse,Gattuso Jean-Pierre",
            
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      "email_authors": 
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      "email_authors": 
            
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"hlavigne@naturalsciences.be,jean-pierre.gattuso@imev-mer.fr",
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"hlavigne@naturalsciences.be,jean-pierre.gattuso@imev-mer.fr",
            
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      "identifier": "https://cran.r-project.org/package=seacarb",
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      "identifier": "https://cran.r-project.org/package=seacarb",
            
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      "identifier_type": "DOI",
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      "identifier_type": "DOI",
            
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      "orcid_authors": ",0000-0002-4533-4114",
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      "orcid_authors": ",0000-0002-4533-4114",
            
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      "relation_type": "References",
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      "relation_type": "References",
            
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      "source": "",
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      "source": "",
            
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      "title": "seacarb: seawater carbonate chemistry with R. R 
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      "title": "seacarb: seawater carbonate chemistry with R. R 
            
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package version 2.4",
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package version 2.4",
            
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      "year": "2011"
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      "year": "2011"
            
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  "repository_name": "PANGAEA (Data Publisher for Earth & 
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Environmental Science)",
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Environmental Science)",
            
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      "subject_area_name": "BiologicalClassification"
            
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      "subject_area_additional": "",
            
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      "subject_area_name": "Biosphere"
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      "subject_area_name": "Biosphere"
            
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      "subject_area_name": "Oceans"
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      "subject_area_name": "Oceans"
            
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      "display_name": "Aquaria 20 L",
            
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    {
            
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      "name": "Micro-nutrients",
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    {
            
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      "display_name": "Open ocean",
            
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    {
            
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      "name": "Pelagos",
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      "name": "Pelagos",
            
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      "state": "active",
            
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      "vocabulary_id": null
            
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    {
            
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      "name": "Photosynthesis",
            
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      "state": "active",
            
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      "vocabulary_id": null
            
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    },
            
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    {
            
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      "display_name": "Primary production-Photosynthesis",
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      "display_name": "Primary production",
            
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      "id": "aca6914d-a728-406d-a5b9-dd9593c21e4e",
            
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      "name": "Primary production-Photosynthesis",
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      "name": "Primary production",
            
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      "state": "active",
            
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      "vocabulary_id": null
            
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    },
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    },
            
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    {
238
    {
            
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      "display_name": "Temperate",
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      "display_name": "Temperate",
            
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      "id": "31aaeb0c-a4a5-4a8e-9c90-be1eff0cac91",
            
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      "name": "Temperate",
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      "name": "Temperate",
            
t
t
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      "state": "active",
            
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      "vocabulary_id": null
            
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    },
            
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    {
            
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      "display_name": "nutrients",
            
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      "id": "46776643-4fc4-4ec7-b6bb-623620baae77",
            
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      "name": "nutrients",
            
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      "state": "active",
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      "state": "active",
            
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      "vocabulary_id": null
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      "vocabulary_id": null
            
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    }
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    }
            
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  ],
252
  ],
            
232
  "title": "Seawater carbonate chemistry and phytoplankton and 
253
  "title": "Seawater carbonate chemistry and phytoplankton and 
            
233
eubacterial community compositions in the northwest subarctic 
254
eubacterial community compositions in the northwest subarctic 
            
234
Pacific",
255
Pacific",
            
235
  "type": "vdataset",
256
  "type": "vdataset",
            
236
  "url": "https://doi.org/10.1594/PANGAEA.820333"
257
  "url": "https://doi.org/10.1594/PANGAEA.820333"
            
237
}
258
}