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{
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{
            
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  "author": "Milde, Raika",
2
  "author": "Milde, Raika",
            
3
  "author_email": "",
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  "author_email": "",
            
4
  "creator_user_id": "17755db4-395a-4b3b-ac09-e8e3484ca700",
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  "creator_user_id": "17755db4-395a-4b3b-ac09-e8e3484ca700",
            
5
  "doi": "10.22000/799",
5
  "doi": "10.22000/799",
            
6
  "doi_date_published": "2022",
6
  "doi_date_published": "2022",
            
7
  "doi_publisher": "",
7
  "doi_publisher": "",
            
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  "doi_status": "True",
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  "doi_status": "True",
            
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  "extra_authors": [
9
  "extra_authors": [
            
10
    {
10
    {
            
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      "extra_author": "Schnabel, Arianne",
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      "extra_author": "Schnabel, Arianne",
            
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      "orcid": "0000-0002-2172-2905"
12
      "orcid": "0000-0002-2172-2905"
            
13
    },
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    },
            
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    {
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    {
            
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      "extra_author": "Ditfe, Toni",
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      "extra_author": "Ditfe, Toni",
            
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      "orcid": "0000-0001-6360-6673"
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      "orcid": "0000-0001-6360-6673"
            
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    },
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    },
            
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    {
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    {
            
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      "extra_author": "Hoehenwarter, Wolfgang",
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      "extra_author": "Hoehenwarter, Wolfgang",
            
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      "orcid": "0000-0002-7669-7524"
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      "orcid": "0000-0002-7669-7524"
            
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    },
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    },
            
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    {
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    {
            
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      "extra_author": "Proksch, Carsten",
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      "extra_author": "Proksch, Carsten",
            
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      "orcid": "0000-0003-4948-8281"
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      "orcid": "0000-0003-4948-8281"
            
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    },
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    },
            
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    {
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    {
            
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      "extra_author": "Westermann, Bernhard",
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      "extra_author": "Westermann, Bernhard",
            
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      "orcid": "0000-0002-6228-5991"
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      "orcid": "0000-0002-6228-5991"
            
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    },
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    },
            
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    {
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    {
            
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      "extra_author": "Vogt, Thomas",
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      "extra_author": "Vogt, Thomas",
            
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      "orcid": "0000-0001-6155-3360"
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      "orcid": "0000-0001-6155-3360"
            
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    }
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    }
            
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  ],
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  ],
            
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  "groups": [],
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  "groups": [],
            
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  "id": "16693d9f-07e1-4ff1-9ab6-87ca53305e1a",
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  "id": "16693d9f-07e1-4ff1-9ab6-87ca53305e1a",
            
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  "isopen": false,
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  "isopen": false,
            
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  "license_id": "CC BY 4.0 Attribution",
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  "license_id": "CC BY 4.0 Attribution",
            
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  "license_title": "CC BY 4.0 Attribution",
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  "license_title": "CC BY 4.0 Attribution",
            
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  "metadata_created": "2023-01-12T13:30:54.420773",
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  "metadata_created": "2023-01-12T13:30:54.420773",
            
t
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  "metadata_modified": "2023-01-12T13:30:54.420780",
t
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  "metadata_modified": "2023-05-02T07:10:05.280841",
            
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  "name": "rdr-doi-10-22000-799",
42
  "name": "rdr-doi-10-22000-799",
            
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  "notes": "Abstract: Capsaicin, produced by diverse Capsicum species 
43
  "notes": "Abstract: Capsaicin, produced by diverse Capsicum species 
            
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is among the world\u2019s most popular spices and of considerable 
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is among the world\u2019s most popular spices and of considerable 
            
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pharmaceutical relevance. Although the capsaicinoid biosynthetic 
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pharmaceutical relevance. Although the capsaicinoid biosynthetic 
            
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pathway has been investigated for decades, several biosynthetic steps 
46
pathway has been investigated for decades, several biosynthetic steps 
            
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have remained partly hypothetical. Genetic evidence suggested that the 
47
have remained partly hypothetical. Genetic evidence suggested that the 
            
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decisive capsaicin synthase is encoded by the Pun1 locus. Yet, genetic 
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decisive capsaicin synthase is encoded by the Pun1 locus. Yet, genetic 
            
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evidence of the Pun1 locus was never corroborated by functionally 
49
evidence of the Pun1 locus was never corroborated by functionally 
            
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active capsaicin synthase that presumably catalyzes amide bond 
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active capsaicin synthase that presumably catalyzes amide bond 
            
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formation between trans 8-methyl-6-nonenoyl-CoA derived from branched 
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formation between trans 8-methyl-6-nonenoyl-CoA derived from branched 
            
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chain amino acid biosynthesis and vanilloylamine derived from the 
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chain amino acid biosynthesis and vanilloylamine derived from the 
            
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phenylpropanoid pathway. In this report, we demonstrate enzymatic 
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phenylpropanoid pathway. In this report, we demonstrate enzymatic 
            
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activity of a recombinant capsaicin synthase encoded by Pun1, 
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activity of a recombinant capsaicin synthase encoded by Pun1, 
            
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functionally expressed in E. coli, and provide information on 
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functionally expressed in E. coli, and provide information on 
            
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substrate specificity and catalytic properties. Recombinant capsaicin 
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substrate specificity and catalytic properties. Recombinant capsaicin 
            
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synthase is specific for selected aliphatic CoA-esters and highly 
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synthase is specific for selected aliphatic CoA-esters and highly 
            
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specific for vanilloylamine. Partly purified from E. coli, the 
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specific for vanilloylamine. Partly purified from E. coli, the 
            
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recombinant active enzyme is a monomeric protein of 51 kDa that is 
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recombinant active enzyme is a monomeric protein of 51 kDa that is 
            
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independent of additional co-factors or associated proteins, as 
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independent of additional co-factors or associated proteins, as 
            
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previously proposed. These data can now be used to design capsaicin 
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previously proposed. These data can now be used to design capsaicin 
            
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synthase variants with different properties and alternative substrate 
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synthase variants with different properties and alternative substrate 
            
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preferences.",
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preferences.",
            
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  "num_resources": 0,
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  "num_resources": 0,
            
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  "num_tags": 8,
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  "num_tags": 8,
            
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  "orcid": "0000-0003-0630-8105",
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  "orcid": "0000-0003-0630-8105",
            
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  "organization": {
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  "organization": {
            
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    "approval_status": "approved",
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    "approval_status": "approved",
            
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    "created": "2023-01-12T13:30:23.238233",
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    "created": "2023-01-12T13:30:23.238233",
            
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    "description": "RADAR (Research Data Repository) is a 
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    "description": "RADAR (Research Data Repository) is a 
            
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cross-disciplinary repository for archiving and publishing research 
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cross-disciplinary repository for archiving and publishing research 
            
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data from completed scientific studies and projects. The focus is on 
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data from completed scientific studies and projects. The focus is on 
            
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research data from subjects that do not yet have their own 
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research data from subjects that do not yet have their own 
            
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discipline-specific infrastructures for research data management. ",
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discipline-specific infrastructures for research data management. ",
            
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    "id": "013c89a9-383c-4200-8baa-0f78bf1d91f9",
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    "id": "013c89a9-383c-4200-8baa-0f78bf1d91f9",
            
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    "image_url": "radar-logo.svg",
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    "image_url": "radar-logo.svg",
            
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    "is_organization": true,
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    "is_organization": true,
            
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    "name": "radar",
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    "name": "radar",
            
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    "state": "active",
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    "state": "active",
            
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    "title": "RADAR",
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    "title": "RADAR",
            
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    "type": "organization"
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    "type": "organization"
            
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  },
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  },
            
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  "owner_org": "013c89a9-383c-4200-8baa-0f78bf1d91f9",
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  "owner_org": "013c89a9-383c-4200-8baa-0f78bf1d91f9",
            
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  "private": false,
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  "private": false,
            
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  "production_year": "2022",
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  "production_year": "2022",
            
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  "publication_year": "2022",
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  "publication_year": "2022",
            
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  "publishers": [
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  "publishers": [
            
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    {
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    {
            
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      "publisher": "Vogt, Thomas"
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      "publisher": "Vogt, Thomas"
            
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    }
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    }
            
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  ],
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  ],
            
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  "relationships_as_object": [],
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  "relationships_as_object": [],
            
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  "relationships_as_subject": [],
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  "relationships_as_subject": [],
            
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  "repository_name": "RADAR (Research Data Repository)",
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  "repository_name": "RADAR (Research Data Repository)",
            
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  "resource_type": "Dataset - chromatogram data (arw files), protein 
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  "resource_type": "Dataset - chromatogram data (arw files), protein 
            
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LC-MS/MS, images (tiff, jpeg), tabular data (csv, xlsx)",
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LC-MS/MS, images (tiff, jpeg), tabular data (csv, xlsx)",
            
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  "resources": [],
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  "resources": [],
            
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  "source_metadata_created": "2022",
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  "source_metadata_created": "2022",
            
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  "source_metadata_modified": "",
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  "source_metadata_modified": "",
            
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  "state": "active",
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  "state": "active",
            
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  "subject_areas": [
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  "subject_areas": [
            
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    {
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    {
            
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      "subject_area_additional": "",
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      "subject_area_additional": "",
            
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      "subject_area_name": "Biochemistry"
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      "subject_area_name": "Biochemistry"
            
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    }
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    }
            
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  ],
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  ],
            
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  "tags": [
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  "tags": [
            
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    {
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    {
            
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      "display_name": "BAHD",
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      "display_name": "BAHD",
            
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      "id": "8a5f3d7c-afd7-44ed-9792-a3ae0a885b35",
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      "id": "8a5f3d7c-afd7-44ed-9792-a3ae0a885b35",
            
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      "name": "BAHD",
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      "name": "BAHD",
            
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      "state": "active",
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      "state": "active",
            
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      "vocabulary_id": null
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      "vocabulary_id": null
            
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    },
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    },
            
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    {
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    {
            
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      "display_name": "Capsaicin",
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      "display_name": "Capsaicin",
            
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      "id": "bbd5ccfb-a796-4f5f-bc95-6bc2f1fa65c9",
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      "id": "bbd5ccfb-a796-4f5f-bc95-6bc2f1fa65c9",
            
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      "name": "Capsaicin",
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      "name": "Capsaicin",
            
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      "state": "active",
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      "state": "active",
            
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      "vocabulary_id": null
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      "vocabulary_id": null
            
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    },
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    },
            
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    {
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    {
            
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      "display_name": "Capsicum",
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      "display_name": "Capsicum",
            
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      "id": "6d9c4e69-e04b-4638-961b-f60439d47b71",
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      "id": "6d9c4e69-e04b-4638-961b-f60439d47b71",
            
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      "name": "Capsicum",
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      "name": "Capsicum",
            
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      "state": "active",
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      "state": "active",
            
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      "vocabulary_id": null
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      "vocabulary_id": null
            
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    },
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    },
            
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    {
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    {
            
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      "display_name": "CoA-ester",
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      "display_name": "CoA-ester",
            
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      "id": "9a4b1c85-6dff-4e36-9965-0bea1040d0af",
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      "id": "9a4b1c85-6dff-4e36-9965-0bea1040d0af",
            
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      "name": "CoA-ester",
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      "name": "CoA-ester",
            
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      "state": "active",
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      "state": "active",
            
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      "vocabulary_id": null
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      "vocabulary_id": null
            
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    },
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    },
            
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    {
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    {
            
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      "display_name": "Pun1",
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      "display_name": "Pun1",
            
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      "id": "0c0118f4-3109-4e15-8d13-6e7271a87ffb",
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      "id": "0c0118f4-3109-4e15-8d13-6e7271a87ffb",
            
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      "name": "Pun1",
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      "name": "Pun1",
            
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      "state": "active",
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      "state": "active",
            
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      "vocabulary_id": null
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      "vocabulary_id": null
            
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    },
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    },
            
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    {
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    {
            
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      "display_name": "acyltransferase",
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      "display_name": "acyltransferase",
            
145
      "id": "228fc1dc-1a69-4d85-aac6-4724f2a4110d",
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      "id": "228fc1dc-1a69-4d85-aac6-4724f2a4110d",
            
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      "name": "acyltransferase",
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      "name": "acyltransferase",
            
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      "state": "active",
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      "state": "active",
            
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      "vocabulary_id": null
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      "vocabulary_id": null
            
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    },
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    },
            
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    {
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    {
            
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      "display_name": "enzyme activity",
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      "display_name": "enzyme activity",
            
152
      "id": "5444a8c0-f0d5-4bc0-8e1b-e482795b60bd",
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      "id": "5444a8c0-f0d5-4bc0-8e1b-e482795b60bd",
            
153
      "name": "enzyme activity",
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      "name": "enzyme activity",
            
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      "state": "active",
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      "state": "active",
            
155
      "vocabulary_id": null
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      "vocabulary_id": null
            
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    },
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    },
            
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    {
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    {
            
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      "display_name": "substrate specificity",
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      "display_name": "substrate specificity",
            
159
      "id": "195b59a9-46ce-4489-83a8-aeb4b1680fed",
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      "id": "195b59a9-46ce-4489-83a8-aeb4b1680fed",
            
160
      "name": "substrate specificity",
160
      "name": "substrate specificity",
            
161
      "state": "active",
161
      "state": "active",
            
162
      "vocabulary_id": null
162
      "vocabulary_id": null
            
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    }
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    }
            
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  ],
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  ],
            
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  "title": "supporting information: chemical synthesis of trans 
165
  "title": "supporting information: chemical synthesis of trans 
            
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8-methyl-6-nonenoyl-coa and functional expression unravel capsaicin 
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8-methyl-6-nonenoyl-coa and functional expression unravel capsaicin 
            
167
synthase activity encoded by the pun1 locus",
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synthase activity encoded by the pun1 locus",
            
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  "type": "vdataset",
168
  "type": "vdataset",
            
169
  "url": "https://doi.org/10.22000/799"
169
  "url": "https://doi.org/10.22000/799"
            
170
}
170
}