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{
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{
            
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  "author": "Dang, Xin",
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  "author": "Dang, Xin",
            
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  "author_email": "",
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  "author_email": "",
            
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  "citation": [],
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  "citation": [],
            
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  "creator_user_id": "17755db4-395a-4b3b-ac09-e8e3484ca700",
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  "creator_user_id": "17755db4-395a-4b3b-ac09-e8e3484ca700",
            
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  "doi": "10.1594/PANGAEA.945551",
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  "doi": "10.1594/PANGAEA.945551",
            
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  "doi_date_published": "2022",
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  "doi_date_published": "2022",
            
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  "doi_publisher": "",
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  "doi_publisher": "",
            
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  "doi_status": "True",
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  "doi_status": "True",
            
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  "extra_authors": [
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  "extra_authors": [
            
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    {
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    {
            
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      "extra_author": "Noor, Zohaib",
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      "extra_author": "Noor, Zohaib",
            
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      "familyName": "Noor",
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      "familyName": "Noor",
            
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      "givenName": "Zohaib",
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      "givenName": "Zohaib",
            
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      "orcid": ""
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      "orcid": ""
            
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    },
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    },
            
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    {
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    {
            
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      "extra_author": "He, Yuanqiu",
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      "extra_author": "He, Yuanqiu",
            
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      "familyName": "He",
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      "familyName": "He",
            
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      "givenName": "Yuanqiu",
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      "givenName": "Yuanqiu",
            
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      "orcid": ""
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      "orcid": ""
            
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    },
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    },
            
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    {
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    {
            
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      "extra_author": "Lim, Yong Kian",
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      "extra_author": "Lim, Yong Kian",
            
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      "familyName": "Lim",
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      "familyName": "Lim",
            
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      "givenName": "Yong Kian",
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      "givenName": "Yong Kian",
            
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      "orcid": "0000-0001-9210-7718"
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      "orcid": "0000-0001-9210-7718"
            
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    },
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    },
            
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    {
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    {
            
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      "extra_author": "Zhang, Yang",
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      "extra_author": "Zhang, Yang",
            
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      "familyName": "Zhang",
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      "familyName": "Zhang",
            
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      "givenName": "Yang",
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      "givenName": "Yang",
            
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      "orcid": "0000-0002-6625-0913"
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      "orcid": "0000-0002-6625-0913"
            
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    },
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    },
            
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    {
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    {
            
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      "extra_author": "Yu, Ziniu",
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      "extra_author": "Yu, Ziniu",
            
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      "familyName": "Yu",
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      "familyName": "Yu",
            
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      "givenName": "Ziniu",
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      "givenName": "Ziniu",
            
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      "orcid": ""
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      "orcid": ""
            
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    },
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    },
            
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    {
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    {
            
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      "extra_author": "Thiyagarajan, Vengatesen",
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      "extra_author": "Thiyagarajan, Vengatesen",
            
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      "familyName": "Thiyagarajan",
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      "familyName": "Thiyagarajan",
            
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      "givenName": "Vengatesen",
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      "givenName": "Vengatesen",
            
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      "orcid": "0000-0002-2062-4799"
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      "orcid": "0000-0002-2062-4799"
            
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    }
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    }
            
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  ],
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  ],
            
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  "familyName": "Dang",
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  "familyName": "Dang",
            
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  "givenName": "Xin",
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  "givenName": "Xin",
            
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  "groups": [],
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  "groups": [],
            
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  "id": "66c3f0c0-d304-4dc9-ad76-41631e412793",
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  "isopen": false,
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  "isopen": false,
            
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  "license_id": "CC-BY-4.0",
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  "license_id": "CC-BY-4.0",
            
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  "license_title": "CC-BY-4.0",
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  "license_title": "CC-BY-4.0",
            
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  "metadata_created": "2024-11-29T11:46:58.182263",
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  "metadata_created": "2024-11-29T11:46:58.182263",
            
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  "metadata_modified": "2024-11-29T11:46:58.182269",
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  "metadata_modified": "2024-11-30T10:48:44.521205",
            
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  "name": "png-doi-10-1594-pangaea-945551",
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  "name": "png-doi-10-1594-pangaea-945551",
            
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  "notes": "The increase of CO2 by anthropogenic activities leads to a 
58
  "notes": "The increase of CO2 by anthropogenic activities leads to a 
            
59
decrease of pH in the ocean surface due to ocean acidification (OA) 
59
decrease of pH in the ocean surface due to ocean acidification (OA) 
            
60
process. Generally, OA not only reduces the rate of calcification in 
60
process. Generally, OA not only reduces the rate of calcification in 
            
61
marine environments but also affects various physiological activities, 
61
marine environments but also affects various physiological activities, 
            
62
especially in calcifiers, including edible oysters. Quantitative 
62
especially in calcifiers, including edible oysters. Quantitative 
            
63
real-time PCR (qRT-PCR) is often used to detect gene expression in 
63
real-time PCR (qRT-PCR) is often used to detect gene expression in 
            
64
response to OA, which relies on the stability of internal control. 
64
response to OA, which relies on the stability of internal control. 
            
65
However, the appropriate internal controls for OA experiments remain 
65
However, the appropriate internal controls for OA experiments remain 
            
66
scarce especially in the marine calcifiers. Hence, this study 
66
scarce especially in the marine calcifiers. Hence, this study 
            
67
developed internal controls for qRT-PCR assays using the Hong Kong 
67
developed internal controls for qRT-PCR assays using the Hong Kong 
            
68
oyster (Crassostrea hongkongensis) as a model to reveal gene 
68
oyster (Crassostrea hongkongensis) as a model to reveal gene 
            
69
expression profile during development under OA. In this study, 17 
69
expression profile during development under OA. In this study, 17 
            
70
housekeeping genes were selected as the possible candidate of the 
70
housekeeping genes were selected as the possible candidate of the 
            
71
internal controls. After a comprehensive interpretation from the 
71
internal controls. After a comprehensive interpretation from the 
            
72
multiple algorithms and software, GAPDH paired with RL23 is 
72
multiple algorithms and software, GAPDH paired with RL23 is 
            
73
recommended for the normalization for planktonic larvae and benthic 
73
recommended for the normalization for planktonic larvae and benthic 
            
74
juveniles, but beyond that, TUBB and EF2 are recommended for 
74
juveniles, but beyond that, TUBB and EF2 are recommended for 
            
75
post-metamorphic stage. Moreover, GAPDH and EF2 were suitable for 
75
post-metamorphic stage. Moreover, GAPDH and EF2 were suitable for 
            
76
various pH treatments, and TUBB, RL35 and RL23 could be the 
76
various pH treatments, and TUBB, RL35 and RL23 could be the 
            
77
alternatives for OA experiments. These results are instrumental for 
77
alternatives for OA experiments. These results are instrumental for 
            
78
the selection of internal control in Crassostrea hongkongensis during 
78
the selection of internal control in Crassostrea hongkongensis during 
            
79
the development, and shed light on other molecular OA experiments in 
79
the development, and shed light on other molecular OA experiments in 
            
80
marine invertebrates for reference.",
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marine invertebrates for reference.",
            
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    "approval_status": "approved",
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    "approval_status": "approved",
            
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    "created": "2024-11-29T11:32:00.143130",
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    "created": "2024-11-30T10:25:26.439072",
            
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    "description": "PANGAEA (Data Publisher for Earth & Environmental 
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    "description": "PANGAEA (Data Publisher for Earth & Environmental 
            
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Science): The information system PANGAEA is operated as an Open Access 
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Science): The information system PANGAEA is operated as an Open Access 
            
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library aimed at archiving, publishing and distributing georeferenced 
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library aimed at archiving, publishing and distributing georeferenced 
            
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data from earth system research. PANGAEA guarantees long-term 
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data from earth system research. PANGAEA guarantees long-term 
            
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availability (greater than 10 years) of its content. PANGAEA is open 
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availability (greater than 10 years) of its content. PANGAEA is open 
            
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to any project, institution, or individual scientist to use or to 
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to any project, institution, or individual scientist to use or to 
            
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archive and publish data. PANGAEA focuses on georeferenced 
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archive and publish data. PANGAEA focuses on georeferenced 
            
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observational data, experimental data, and models/simulations. 
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observational data, experimental data, and models/simulations. 
            
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Citability, comprehensive metadata descriptions, interoperability of 
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Citability, comprehensive metadata descriptions, interoperability of 
            
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data and metadata, a high degree of structural and semantic 
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data and metadata, a high degree of structural and semantic 
            
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harmonization of the data inventory as well as the commitment of the 
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harmonization of the data inventory as well as the commitment of the 
            
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hosting institutions ensures FAIRness of archived data.",
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hosting institutions ensures FAIRness of archived data.",
            
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    "state": "active",
            
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    "title": "PANGAEA (Biosphere)",
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    "title": "PANGAEA (Ecology)",
            
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  "publication_year": "2022",
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  "publication_year": "2022",
            
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  "related_identifiers": [
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  "related_identifiers": [
            
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    {
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    {
            
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      "authors": "Dang Xin,Noor Zohaib,He Yuanqiu,Lim Yong Kian,Zhang 
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      "authors": "Dang Xin,Noor Zohaib,He Yuanqiu,Lim Yong Kian,Zhang 
            
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Yang,Yu Ziniu,Thiyagarajan Vengatesen,Gattuso Jean-Pierre,Epitalon 
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Yang,Yu Ziniu,Thiyagarajan Vengatesen,Gattuso Jean-Pierre,Epitalon 
            
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Jean-Marie,Lavigne H\u00e9lo\u00efse,Orr James",
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Jean-Marie,Lavigne H\u00e9lo\u00efse,Orr James",
            
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      "email_authors": 
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      "email_authors": 
            
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tuso@imev-mer.fr,,hlavigne@naturalsciences.be,James.Orr@lsce.ipsl.fr",
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tuso@imev-mer.fr,,hlavigne@naturalsciences.be,James.Orr@lsce.ipsl.fr",
            
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      "identifier": "https://doi.org/10.1016/j.jembe.2021.151683",
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      "identifier": "https://doi.org/10.1016/j.jembe.2021.151683",
            
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      "identifier_type": "DOI",
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      "identifier_type": "DOI",
            
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      "orcid_authors": 
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      "orcid_authors": 
            
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7718,0000-0002-6625-0913,,0000-0002-2062-4799,0000-0002-4533-4114,,,",
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7718,0000-0002-6625-0913,,0000-0002-2062-4799,0000-0002-4533-4114,,,",
            
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      "relation_type": "References",
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      "relation_type": "References",
            
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      "source": "Journal of Experimental Marine Biology and Ecology",
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      "source": "Journal of Experimental Marine Biology and Ecology",
            
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      "title": "Internal controls for quantitative RT-PCR analysis of 
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      "title": "Internal controls for quantitative RT-PCR analysis of 
            
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gene expression in response to ocean acidification in edible oysters",
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gene expression in response to ocean acidification in edible oysters",
            
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      "year": "2022"
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      "year": "2022"
            
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    },
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    },
            
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    {
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    {
            
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      "authors": "Dang Xin,Noor Zohaib,He Yuanqiu,Lim Yong Kian,Zhang 
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      "authors": "Dang Xin,Noor Zohaib,He Yuanqiu,Lim Yong Kian,Zhang 
            
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Yang,Yu Ziniu,Thiyagarajan Vengatesen,Gattuso Jean-Pierre,Epitalon 
129
Yang,Yu Ziniu,Thiyagarajan Vengatesen,Gattuso Jean-Pierre,Epitalon 
            
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Jean-Marie,Lavigne H\u00e9lo\u00efse,Orr James",
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Jean-Marie,Lavigne H\u00e9lo\u00efse,Orr James",
            
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      "email_authors": 
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      "email_authors": 
            
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tuso@imev-mer.fr,,hlavigne@naturalsciences.be,James.Orr@lsce.ipsl.fr",
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tuso@imev-mer.fr,,hlavigne@naturalsciences.be,James.Orr@lsce.ipsl.fr",
            
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      "identifier": 
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      "identifier": 
            
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"https://cran.r-project.org/web/packages/seacarb/index.html",
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"https://cran.r-project.org/web/packages/seacarb/index.html",
            
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      "identifier_type": "DOI",
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      "identifier_type": "DOI",
            
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      "orcid_authors": 
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      "orcid_authors": 
            
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7718,0000-0002-6625-0913,,0000-0002-2062-4799,0000-0002-4533-4114,,,",
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7718,0000-0002-6625-0913,,0000-0002-2062-4799,0000-0002-4533-4114,,,",
            
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      "relation_type": "References",
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      "relation_type": "References",
            
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      "source": "",
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      "source": "",
            
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      "title": "seacarb: seawater carbonate chemistry with R. R 
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      "title": "seacarb: seawater carbonate chemistry with R. R 
            
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package version 3.2.16",
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package version 3.2.16",
            
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      "year": "2021"
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      "year": "2021"
            
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    }
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Environmental Science)",
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Environmental Science)",
            
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Dang_etal_2022",
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  "source_metadata_created": "2022",
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  "source_metadata_created": "2022",
            
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      "subject_area_name": "BiologicalClassification"
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      "subject_area_name": "BiologicalClassification"
            
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    },
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    },
            
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    {
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    {
            
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      "subject_area_additional": "",
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      "subject_area_additional": "",
            
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      "subject_area_name": "Biosphere"
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      "subject_area_name": "Biosphere"
            
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    },
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    },
            
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    {
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    {
            
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      "subject_area_additional": "",
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      "subject_area_additional": "",
            
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      "subject_area_name": "Chemistry"
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      "subject_area_name": "Chemistry"
            
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    },
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    },
            
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    {
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    {
            
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      "subject_area_additional": "",
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      "subject_area_additional": "",
            
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      "subject_area_name": "Ecology"
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      "subject_area_name": "Ecology"
            
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    },
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    },
            
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    {
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    {
            
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      "subject_area_additional": "",
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      "subject_area_additional": "",
            
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      "subject_area_name": "Oceans"
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      "subject_area_name": "Oceans"
            
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    }
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    }
            
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  ],
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  ],
            
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  "tags": [
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  "tags": [
            
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    {
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    {
            
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      "display_name": "1000 L or  1 m2",
            
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      "display_name": "Animalia",
            
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      "name": "Animalia",
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      "name": "Animalia",
            
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      "state": "active",
            
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    },
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    },
            
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    {
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    {
            
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      "display_name": "Benthic animals",
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      "display_name": "Benthic animals",
            
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      "id": "ba711ac9-d2d3-4cff-8023-8b85163344bb",
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      "id": "ba711ac9-d2d3-4cff-8023-8b85163344bb",
            
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      "name": "Benthic animals",
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      "name": "Benthic animals",
            
189
      "state": "active",
196
      "state": "active",
            
190
      "vocabulary_id": null
197
      "vocabulary_id": null
            
191
    },
198
    },
            
192
    {
199
    {
            
193
      "display_name": "Benthos",
200
      "display_name": "Benthos",
            
194
      "id": "55bc70f2-ff73-4d9a-89f6-16358d0d9b87",
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      "id": "55bc70f2-ff73-4d9a-89f6-16358d0d9b87",
            
195
      "name": "Benthos",
202
      "name": "Benthos",
            
196
      "state": "active",
203
      "state": "active",
            
197
      "vocabulary_id": null
204
      "vocabulary_id": null
            
198
    },
205
    },
            
199
    {
206
    {
            
200
      "display_name": "Coast and continental shelf",
207
      "display_name": "Coast and continental shelf",
            
201
      "id": "58eb1929-34b5-4fd9-ac44-824f0e51de40",
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      "id": "58eb1929-34b5-4fd9-ac44-824f0e51de40",
            
202
      "name": "Coast and continental shelf",
209
      "name": "Coast and continental shelf",
            
203
      "state": "active",
210
      "state": "active",
            
204
      "vocabulary_id": null
211
      "vocabulary_id": null
            
205
    },
212
    },
            
206
    {
213
    {
            
t
207
      "display_name": "Containers and aquaria 20-1000 L or  1 m2",
t
214
      "display_name": "Containers and aquaria 20",
            
208
      "id": "a9d9036d-3fe8-4fb1-bcb9-07b8a3a3fe11",
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      "id": "18d57eca-7aec-4d8a-9a57-92bf32a45d53",
            
209
      "name": "Containers and aquaria 20-1000 L or  1 m2",
216
      "name": "Containers and aquaria 20",
            
210
      "state": "active",
217
      "state": "active",
            
211
      "vocabulary_id": null
218
      "vocabulary_id": null
            
212
    },
219
    },
            
213
    {
220
    {
            
214
      "display_name": "Gene expression incl. proteomics",
221
      "display_name": "Gene expression incl. proteomics",
            
215
      "id": "a266e76f-1f2a-427a-97e6-8e248d591f17",
222
      "id": "a266e76f-1f2a-427a-97e6-8e248d591f17",
            
216
      "name": "Gene expression incl. proteomics",
223
      "name": "Gene expression incl. proteomics",
            
217
      "state": "active",
224
      "state": "active",
            
218
      "vocabulary_id": null
225
      "vocabulary_id": null
            
219
    },
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    },
            
220
    {
227
    {
            
221
      "display_name": "Laboratory experiment",
228
      "display_name": "Laboratory experiment",
            
222
      "id": "c6c00754-c769-4932-a517-bff2c9512884",
229
      "id": "c6c00754-c769-4932-a517-bff2c9512884",
            
223
      "name": "Laboratory experiment",
230
      "name": "Laboratory experiment",
            
224
      "state": "active",
231
      "state": "active",
            
225
      "vocabulary_id": null
232
      "vocabulary_id": null
            
226
    },
233
    },
            
227
    {
234
    {
            
228
      "display_name": "Magallana hongkongensis",
235
      "display_name": "Magallana hongkongensis",
            
229
      "id": "e9e78bd8-2e98-4d56-89e2-a0f73eb5c15b",
236
      "id": "e9e78bd8-2e98-4d56-89e2-a0f73eb5c15b",
            
230
      "name": "Magallana hongkongensis",
237
      "name": "Magallana hongkongensis",
            
231
      "state": "active",
238
      "state": "active",
            
232
      "vocabulary_id": null
239
      "vocabulary_id": null
            
233
    },
240
    },
            
234
    {
241
    {
            
235
      "display_name": "Mollusca",
242
      "display_name": "Mollusca",
            
236
      "id": "006d5f6d-11b3-4c34-a35a-879d7cd074c8",
243
      "id": "006d5f6d-11b3-4c34-a35a-879d7cd074c8",
            
237
      "name": "Mollusca",
244
      "name": "Mollusca",
            
238
      "state": "active",
245
      "state": "active",
            
239
      "vocabulary_id": null
246
      "vocabulary_id": null
            
240
    },
247
    },
            
241
    {
248
    {
            
242
      "display_name": "North Pacific",
249
      "display_name": "North Pacific",
            
243
      "id": "efbdbaff-aa72-4a57-8e03-e9f84253d644",
250
      "id": "efbdbaff-aa72-4a57-8e03-e9f84253d644",
            
244
      "name": "North Pacific",
251
      "name": "North Pacific",
            
245
      "state": "active",
252
      "state": "active",
            
246
      "vocabulary_id": null
253
      "vocabulary_id": null
            
247
    },
254
    },
            
248
    {
255
    {
            
249
      "display_name": "Single species",
256
      "display_name": "Single species",
            
250
      "id": "88de69be-3bdd-4cf8-b6a1-386d72c5888d",
257
      "id": "88de69be-3bdd-4cf8-b6a1-386d72c5888d",
            
251
      "name": "Single species",
258
      "name": "Single species",
            
252
      "state": "active",
259
      "state": "active",
            
253
      "vocabulary_id": null
260
      "vocabulary_id": null
            
254
    },
261
    },
            
255
    {
262
    {
            
256
      "display_name": "Tropical",
263
      "display_name": "Tropical",
            
257
      "id": "db8bd80f-8578-455b-acc6-6adbeae406b8",
264
      "id": "db8bd80f-8578-455b-acc6-6adbeae406b8",
            
258
      "name": "Tropical",
265
      "name": "Tropical",
            
259
      "state": "active",
266
      "state": "active",
            
260
      "vocabulary_id": null
267
      "vocabulary_id": null
            
261
    }
268
    }
            
262
  ],
269
  ],
            
263
  "title": "Seawater carbonate chemistry and gene expression stability 
270
  "title": "Seawater carbonate chemistry and gene expression stability 
            
264
of Hong Kong oyster (Crassostrea hongkongensis)",
271
of Hong Kong oyster (Crassostrea hongkongensis)",
            
265
  "type": "vdataset",
272
  "type": "vdataset",
            
266
  "url": "https://doi.org/10.1594/PANGAEA.945551"
273
  "url": "https://doi.org/10.1594/PANGAEA.945551"
            
267
}
274
}