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{
            
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  "author": "Jana Schellenberg, Michaela Dehne",
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  "author": "Jana Schellenberg",
            
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  "author_email": "",
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  "author_email": "",
            
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  "creator_user_id": "17755db4-395a-4b3b-ac09-e8e3484ca700",
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  "creator_user_id": "17755db4-395a-4b3b-ac09-e8e3484ca700",
            
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  "doi": "10.25835/nxrqvp0w",
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  "doi": "10.25835/nxrqvp0w",
            
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  "doi_date_published": "2023-04-21",
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  "doi_date_published": "2023-04-21",
            
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  "doi_publisher": "LUIS",
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  "doi_publisher": "LUIS",
            
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  "doi_status": "true",
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  "doi_status": "true",
            
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  "domain": "https://data.uni-hannover.de",
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  "domain": "https://data.uni-hannover.de",
            
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  "extra_authors": [
            
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    {
            
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      "extra_author": " Michaela Dehne"
            
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    }
            
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  ],
            
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  "groups": [],
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  "groups": [],
            
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  "have_copyright": "Yes",
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  "have_copyright": "Yes",
            
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  "isopen": false,
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  "isopen": false,
            
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  "license_id": "CC0-1.0",
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  "license_id": "CC0-1.0",
            
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  "license_title": "CC0-1.0",
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  "license_title": "CC0-1.0",
            
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  "maintainer": "D\u00f6rte Solle",
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  "maintainer": "D\u00f6rte Solle",
            
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  "maintainer_email": "",
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  "maintainer_email": "",
            
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  "metadata_created": "2023-05-02T07:05:19.013601",
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  "metadata_created": "2023-05-02T07:05:19.013601",
            
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  "metadata_modified": "2023-05-02T07:05:19.013606",
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  "metadata_modified": "2023-08-04T08:46:22.103178",
            
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  "name": 
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  "name": 
            
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ss-using-a-microfluidic-spiral-separator-with-web-based-flow-control",
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ss-using-a-microfluidic-spiral-separator-with-web-based-flow-control",
            
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  "notes": "In an ongoing effort to increase product yields of CHO 
27
  "notes": "In an ongoing effort to increase product yields of CHO 
            
23
cells, a 3D-printed microfluidic spiral separator for plug and play 
28
cells, a 3D-printed microfluidic spiral separator for plug and play 
            
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cell retention was combined with a small-scale bioreactor system. In 
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cell retention was combined with a small-scale bioreactor system. In 
            
25
addition, this system includes automatic flushing, web-based control, 
30
addition, this system includes automatic flushing, web-based control, 
            
26
and a mobile status notification.\r\nA proof-of-concept cultivation 
31
and a mobile status notification.\r\nA proof-of-concept cultivation 
            
27
over 530 h at viable cell concentrations between 10-17 x 106 cells/mL 
32
over 530 h at viable cell concentrations between 10-17 x 106 cells/mL 
            
28
in a hybrid mode (with alternating cell retention and cell bleed 
33
in a hybrid mode (with alternating cell retention and cell bleed 
            
29
phases) successfully reduced both shear stress and microfluidic 
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phases) successfully reduced both shear stress and microfluidic 
            
30
channel blockage. In addition to a retention efficiency of almost 100 
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channel blockage. In addition to a retention efficiency of almost 100 
            
31
%, this set-up also increased cell viability within the bioreactor by 
36
%, this set-up also increased cell viability within the bioreactor by 
            
32
separating dead cells and cell debris into the cell-free fraction. 
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separating dead cells and cell debris into the cell-free fraction. 
            
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Moreover, no retention of the product was detected.",
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Moreover, no retention of the product was detected.",
            
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  "organization": {
            
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    "approval_status": "approved",
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    "approval_status": "approved",
            
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    "created": "2021-10-14T10:15:53.756874",
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    "created": "2021-10-14T10:15:53.756874",
            
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  "repository_name": "Leibniz University Hannover",
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  "repository_name": "Leibniz University Hannover",
            
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  "source_metadata_modified": "2023-04-24T08:19:36.976702",
            
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  "state": "active",
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  "state": "active",
            
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  "tags": [
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  "tags": [
            
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    {
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    {
            
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      "display_name": "3D-print",
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      "display_name": "3D-print",
            
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      "id": "ad5bb3f9-0e25-492e-aed7-b01aae7a9484",
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    },
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    },
            
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    {
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    {
            
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      "display_name": "CHO",
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      "display_name": "CHO",
            
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      "id": "76b6c05d-ec66-4270-89a6-d865ff2ec251",
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      "id": "76b6c05d-ec66-4270-89a6-d865ff2ec251",
            
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      "name": "CHO",
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      "name": "CHO",
            
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      "state": "active",
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      "state": "active",
            
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      "vocabulary_id": null
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      "vocabulary_id": null
            
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    },
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    },
            
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    {
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    {
            
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      "display_name": "mAb",
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      "display_name": "mAb",
            
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      "id": "ac73686d-7f7c-4df7-9bc5-658fc3a5605a",
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      "id": "ac73686d-7f7c-4df7-9bc5-658fc3a5605a",
            
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      "name": "mAb",
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      "name": "mAb",
            
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      "state": "active",
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      "state": "active",
            
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      "vocabulary_id": null
            
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    },
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    },
            
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    {
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    {
            
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      "display_name": "mikrofluidic spiral separator",
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      "display_name": "mikrofluidic spiral separator",
            
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      "id": "caac8485-9c15-4337-8fad-78e1b702507f",
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      "id": "caac8485-9c15-4337-8fad-78e1b702507f",
            
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      "name": "mikrofluidic spiral separator",
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      "name": "mikrofluidic spiral separator",
            
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      "state": "active",
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      "state": "active",
            
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      "vocabulary_id": null
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      "vocabulary_id": null
            
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    }
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    }
            
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  ],
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  ],
            
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  "terms_of_usage": "Yes",
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  "terms_of_usage": "Yes",
            
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  "title": "CHO Perfusion Process using a microfluidic spiral 
139
  "title": "CHO Perfusion Process using a microfluidic spiral 
            
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separator with web-based flow control",
140
separator with web-based flow control",
            
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  "type": "vdataset",
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  "type": "vdataset",
            
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  "url": 
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  "url": 
            
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ss-using-a-microfluidic-spiral-separator-with-web-based-flow-control",
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ss-using-a-microfluidic-spiral-separator-with-web-based-flow-control",
            
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  "version": "1.0"
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  "version": "1.0"
            
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}
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}