Changes

              
    

          
          
        
        

            
f
1
{
f
1
{
            
2
  "author": "Schiffer, Melanie",
2
  "author": "Schiffer, Melanie",
            
3
  "author_email": "",
3
  "author_email": "",
            
4
  "citation": [],
4
  "citation": [],
            
5
  "creator_user_id": "17755db4-395a-4b3b-ac09-e8e3484ca700",
5
  "creator_user_id": "17755db4-395a-4b3b-ac09-e8e3484ca700",
            
6
  "doi": "10.1594/PANGAEA.846762",
6
  "doi": "10.1594/PANGAEA.846762",
            
7
  "doi_date_published": "2014",
7
  "doi_date_published": "2014",
            
8
  "doi_publisher": "",
8
  "doi_publisher": "",
            
9
  "doi_status": "True",
9
  "doi_status": "True",
            
10
  "familyName": "Schiffer",
10
  "familyName": "Schiffer",
            
11
  "givenName": "Melanie",
11
  "givenName": "Melanie",
            
12
  "groups": [],
12
  "groups": [],
            
13
  "id": "b502d8b7-27c6-4128-90fe-a322de9ee1e8",
13
  "id": "b502d8b7-27c6-4128-90fe-a322de9ee1e8",
            
14
  "isopen": false,
14
  "isopen": false,
            
15
  "license_id": "CC-BY-3.0",
15
  "license_id": "CC-BY-3.0",
            
16
  "license_title": "CC-BY-3.0",
16
  "license_title": "CC-BY-3.0",
            
17
  "metadata_created": "2024-11-29T11:45:23.869842",
17
  "metadata_created": "2024-11-29T11:45:23.869842",
            
n
18
  "metadata_modified": "2024-11-29T11:45:23.869849",
n
18
  "metadata_modified": "2024-11-30T14:30:24.510072",
            
19
  "name": "png-doi-10-1594-pangaea-846762",
19
  "name": "png-doi-10-1594-pangaea-846762",
            
20
  "notes": "Exposure to elevated seawater PCO2 limits the thermal 
20
  "notes": "Exposure to elevated seawater PCO2 limits the thermal 
            
21
tolerance of crustaceans but the underlying mechanisms have not been 
21
tolerance of crustaceans but the underlying mechanisms have not been 
            
22
comprehensively explored. Larval stages of crustaceans are even more 
22
comprehensively explored. Larval stages of crustaceans are even more 
            
23
sensitive to environmental hypercapnia and possess narrower thermal 
23
sensitive to environmental hypercapnia and possess narrower thermal 
            
24
windows than adults. In a mechanistic approach, we analysed the impact 
24
windows than adults. In a mechanistic approach, we analysed the impact 
            
25
of high seawater CO2 on parameters at different levels of biological 
25
of high seawater CO2 on parameters at different levels of biological 
            
26
organization, from the molecular to the whole animal level. At the 
26
organization, from the molecular to the whole animal level. At the 
            
27
whole animal level we measured oxygen consumption, heart rate and 
27
whole animal level we measured oxygen consumption, heart rate and 
            
28
activity during acute warming in zoea and megalopa larvae of the 
28
activity during acute warming in zoea and megalopa larvae of the 
            
29
spider crab Hyas araneus exposed to different levels of seawater PCO2. 
29
spider crab Hyas araneus exposed to different levels of seawater PCO2. 
            
30
Furthermore, the expression of genes responsible for acid-base 
30
Furthermore, the expression of genes responsible for acid-base 
            
31
regulation and mitochondrial energy metabolism, and cellular responses 
31
regulation and mitochondrial energy metabolism, and cellular responses 
            
32
to thermal stress (e.g. the heat shock response) was analysed before 
32
to thermal stress (e.g. the heat shock response) was analysed before 
            
33
and after larvae were heat shocked byrapidly raising the seawater 
33
and after larvae were heat shocked byrapidly raising the seawater 
            
34
temperature from 10\u00b0C rearing temperature to 20\u00b0C. Zoea 
34
temperature from 10\u00b0C rearing temperature to 20\u00b0C. Zoea 
            
35
larvae showed a high heat tolerance, which decreased at elevated 
35
larvae showed a high heat tolerance, which decreased at elevated 
            
36
seawater PCO2, while the already low heat tolerance of megalopa larvae 
36
seawater PCO2, while the already low heat tolerance of megalopa larvae 
            
37
was not limited further by hypercapnic exposure. There was a combined 
37
was not limited further by hypercapnic exposure. There was a combined 
            
38
effect of elevated seawater CO2 and heat shock in zoea larvae causing 
38
effect of elevated seawater CO2 and heat shock in zoea larvae causing 
            
39
elevated transcript levels of heat shock proteins. In all three larval 
39
elevated transcript levels of heat shock proteins. In all three larval 
            
40
stages, hypercapnic exposure elicited an up-regulation of genes 
40
stages, hypercapnic exposure elicited an up-regulation of genes 
            
41
involved in oxidative phosphorylation, which was, however, not 
41
involved in oxidative phosphorylation, which was, however, not 
            
42
accompanied by increased energetic demands. The combined effect of 
42
accompanied by increased energetic demands. The combined effect of 
            
43
seawater CO2 and heat shock on the gene expression of heat shock 
43
seawater CO2 and heat shock on the gene expression of heat shock 
            
44
proteins reflects the downward shift in thermal limits seen on the 
44
proteins reflects the downward shift in thermal limits seen on the 
            
45
whole animal level and indicates an associated capacity to elicit 
45
whole animal level and indicates an associated capacity to elicit 
            
46
passive thermal tolerance. The up-regulation of genes involved in 
46
passive thermal tolerance. The up-regulation of genes involved in 
            
47
oxidative phosphorylation might compensate for enzyme activities being 
47
oxidative phosphorylation might compensate for enzyme activities being 
            
48
lowered through bicarbonate inhibition and maintain larval standard 
48
lowered through bicarbonate inhibition and maintain larval standard 
            
49
metabolic rates at high seawater CO2 levels. The present study 
49
metabolic rates at high seawater CO2 levels. The present study 
            
50
underlines the necessity to align transcriptomic data with 
50
underlines the necessity to align transcriptomic data with 
            
51
physiological responses when addressing mechanisms affected by an 
51
physiological responses when addressing mechanisms affected by an 
            
52
interaction of elevated seawater PCO2 and temperature extremes.",
52
interaction of elevated seawater PCO2 and temperature extremes.",
            
53
  "num_resources": 0,
53
  "num_resources": 0,
            
n
54
  "num_tags": 16,
n
54
  "num_tags": 18,
            
55
  "orcid": "",
55
  "orcid": "",
            
56
  "organization": {
56
  "organization": {
            
57
    "approval_status": "approved",
57
    "approval_status": "approved",
            
n
58
    "created": "2024-11-29T11:32:00.143130",
n
58
    "created": "2024-11-30T14:19:14.458899",
            
59
    "description": "PANGAEA (Data Publisher for Earth & Environmental 
59
    "description": "PANGAEA (Data Publisher for Earth & Environmental 
            
60
Science): The information system PANGAEA is operated as an Open Access 
60
Science): The information system PANGAEA is operated as an Open Access 
            
61
library aimed at archiving, publishing and distributing georeferenced 
61
library aimed at archiving, publishing and distributing georeferenced 
            
62
data from earth system research. PANGAEA guarantees long-term 
62
data from earth system research. PANGAEA guarantees long-term 
            
63
availability (greater than 10 years) of its content. PANGAEA is open 
63
availability (greater than 10 years) of its content. PANGAEA is open 
            
64
to any project, institution, or individual scientist to use or to 
64
to any project, institution, or individual scientist to use or to 
            
65
archive and publish data. PANGAEA focuses on georeferenced 
65
archive and publish data. PANGAEA focuses on georeferenced 
            
66
observational data, experimental data, and models/simulations. 
66
observational data, experimental data, and models/simulations. 
            
67
Citability, comprehensive metadata descriptions, interoperability of 
67
Citability, comprehensive metadata descriptions, interoperability of 
            
68
data and metadata, a high degree of structural and semantic 
68
data and metadata, a high degree of structural and semantic 
            
69
harmonization of the data inventory as well as the commitment of the 
69
harmonization of the data inventory as well as the commitment of the 
            
70
hosting institutions ensures FAIRness of archived data.",
70
hosting institutions ensures FAIRness of archived data.",
            
n
71
    "id": "3226ef9c-20d1-43fd-ba8f-fa35c8c9fb5d",
n
71
    "id": "e4c6c709-6080-430f-8979-e57014b1b0ee",
            
72
    "image_url": "pangaea_topicbiosphere.png",
72
    "image_url": "pangaea_topicbiologicalclassification.png",
            
73
    "is_organization": true,
73
    "is_organization": true,
            
n
74
    "name": "pangaea_biosphere",
n
74
    "name": "pangaea_biologicalclassification",
            
75
    "state": "active",
75
    "state": "active",
            
n
76
    "title": "PANGAEA (Biosphere)",
n
76
    "title": "PANGAEA (Biological Classification)",
            
77
    "type": "organization"
77
    "type": "organization"
            
78
  },
78
  },
            
n
79
  "owner_org": "3226ef9c-20d1-43fd-ba8f-fa35c8c9fb5d",
n
79
  "owner_org": "e4c6c709-6080-430f-8979-e57014b1b0ee",
            
80
  "private": false,
80
  "private": false,
            
81
  "publication_year": "2014",
81
  "publication_year": "2014",
            
82
  "related_identifiers": [
82
  "related_identifiers": [
            
83
    {
83
    {
            
84
      "authors": "Schiffer Melanie,Harms Lars,Lucassen Magnus,Mark 
84
      "authors": "Schiffer Melanie,Harms Lars,Lucassen Magnus,Mark 
            
85
Felix Christopher,P\u00f6rtner Hans-Otto,Storch Daniela",
85
Felix Christopher,P\u00f6rtner Hans-Otto,Storch Daniela",
            
86
      "email_authors": 
86
      "email_authors": 
            
87
istopher.Mark@awi.de,hans-otto.poertner@awi.de,Daniela.Storch@awi.de",
87
istopher.Mark@awi.de,hans-otto.poertner@awi.de,Daniela.Storch@awi.de",
            
88
      "identifier": "https://doi.org/10.1186/s12983-014-0087-4",
88
      "identifier": "https://doi.org/10.1186/s12983-014-0087-4",
            
89
      "identifier_type": "DOI",
89
      "identifier_type": "DOI",
            
90
      "orcid_authors": 
90
      "orcid_authors": 
            
91
276-4781,0000-0002-5586-6704,0000-0001-6535-6575,0000-0003-3090-7554",
91
276-4781,0000-0002-5586-6704,0000-0001-6535-6575,0000-0003-3090-7554",
            
92
      "relation_type": "IsSupplementTo",
92
      "relation_type": "IsSupplementTo",
            
93
      "source": "Frontiers in Zoology",
93
      "source": "Frontiers in Zoology",
            
94
      "title": "Temperature tolerance of different larval stages of 
94
      "title": "Temperature tolerance of different larval stages of 
            
95
the spider crab Hyas araneus exposed to elevated seawater PCO2",
95
the spider crab Hyas araneus exposed to elevated seawater PCO2",
            
96
      "year": "2014"
96
      "year": "2014"
            
97
    },
97
    },
            
98
    {
98
    {
            
99
      "authors": "Gattuso Jean-Pierre,Epitalon Jean-Marie,Lavigne 
99
      "authors": "Gattuso Jean-Pierre,Epitalon Jean-Marie,Lavigne 
            
100
H\u00e9lo\u00efse",
100
H\u00e9lo\u00efse",
            
101
      "email_authors": 
101
      "email_authors": 
            
102
"jean-pierre.gattuso@imev-mer.fr,,hlavigne@naturalsciences.be",
102
"jean-pierre.gattuso@imev-mer.fr,,hlavigne@naturalsciences.be",
            
103
      "identifier": "https://cran.r-project.org/package=seacarb",
103
      "identifier": "https://cran.r-project.org/package=seacarb",
            
104
      "identifier_type": "DOI",
104
      "identifier_type": "DOI",
            
105
      "orcid_authors": "0000-0002-4533-4114,,",
105
      "orcid_authors": "0000-0002-4533-4114,,",
            
106
      "relation_type": "References",
106
      "relation_type": "References",
            
107
      "source": "",
107
      "source": "",
            
108
      "title": "seacarb: seawater carbonate chemistry with R. R 
108
      "title": "seacarb: seawater carbonate chemistry with R. R 
            
109
package version 3.0.6",
109
package version 3.0.6",
            
110
      "year": "2015"
110
      "year": "2015"
            
111
    }
111
    }
            
112
  ],
112
  ],
            
113
  "relationships_as_object": [],
113
  "relationships_as_object": [],
            
114
  "relationships_as_subject": [],
114
  "relationships_as_subject": [],
            
115
  "repository_name": "PANGAEA (Data Publisher for Earth & 
115
  "repository_name": "PANGAEA (Data Publisher for Earth & 
            
116
Environmental Science)",
116
Environmental Science)",
            
117
  "resource_type": "text/tab-separated-values - filename: 
117
  "resource_type": "text/tab-separated-values - filename: 
            
118
Schiffer_2014",
118
Schiffer_2014",
            
119
  "resources": [],
119
  "resources": [],
            
120
  "source_metadata_created": "2014",
120
  "source_metadata_created": "2014",
            
121
  "source_metadata_modified": "",
121
  "source_metadata_modified": "",
            
122
  "state": "active",
122
  "state": "active",
            
123
  "subject_areas": [
123
  "subject_areas": [
            
124
    {
124
    {
            
125
      "subject_area_additional": "",
125
      "subject_area_additional": "",
            
126
      "subject_area_name": "BiologicalClassification"
126
      "subject_area_name": "BiologicalClassification"
            
127
    },
127
    },
            
128
    {
128
    {
            
129
      "subject_area_additional": "",
129
      "subject_area_additional": "",
            
130
      "subject_area_name": "Biosphere"
130
      "subject_area_name": "Biosphere"
            
131
    },
131
    },
            
132
    {
132
    {
            
133
      "subject_area_additional": "",
133
      "subject_area_additional": "",
            
134
      "subject_area_name": "Chemistry"
134
      "subject_area_name": "Chemistry"
            
135
    }
135
    }
            
136
  ],
136
  ],
            
137
  "tags": [
137
  "tags": [
            
138
    {
138
    {
            
139
      "display_name": "Animalia",
139
      "display_name": "Animalia",
            
140
      "id": "e645e194-a2f7-40f1-ae7c-c1496be6f6bf",
140
      "id": "e645e194-a2f7-40f1-ae7c-c1496be6f6bf",
            
141
      "name": "Animalia",
141
      "name": "Animalia",
            
142
      "state": "active",
142
      "state": "active",
            
143
      "vocabulary_id": null
143
      "vocabulary_id": null
            
144
    },
144
    },
            
145
    {
145
    {
            
n
n
146
      "display_name": "Aquaria 20 L",
            
147
      "id": "62c412c1-2294-406a-ad79-183330400def",
            
148
      "name": "Aquaria 20 L",
            
149
      "state": "active",
            
150
      "vocabulary_id": null
            
151
    },
            
152
    {
            
146
      "display_name": "Arthropoda",
153
      "display_name": "Arthropoda",
            
147
      "id": "34a6ba80-52d8-40d3-a5e5-38a9c5abeba1",
154
      "id": "34a6ba80-52d8-40d3-a5e5-38a9c5abeba1",
            
148
      "name": "Arthropoda",
155
      "name": "Arthropoda",
            
149
      "state": "active",
156
      "state": "active",
            
150
      "vocabulary_id": null
157
      "vocabulary_id": null
            
151
    },
158
    },
            
152
    {
159
    {
            
153
      "display_name": "Behaviour",
160
      "display_name": "Behaviour",
            
154
      "id": "997ae82e-dd3f-414b-8131-7288c916ef2b",
161
      "id": "997ae82e-dd3f-414b-8131-7288c916ef2b",
            
155
      "name": "Behaviour",
162
      "name": "Behaviour",
            
156
      "state": "active",
163
      "state": "active",
            
157
      "vocabulary_id": null
164
      "vocabulary_id": null
            
158
    },
165
    },
            
159
    {
166
    {
            
n
160
      "display_name": "Bottles or small containers-Aquaria 20 L",
n
167
      "display_name": "Bottles or small containers",
            
161
      "id": "be154617-73a3-4ed4-b54f-69e22323c12a",
168
      "id": "ba227243-af91-4efc-8086-790112e90e09",
            
162
      "name": "Bottles or small containers-Aquaria 20 L",
169
      "name": "Bottles or small containers",
            
163
      "state": "active",
170
      "state": "active",
            
164
      "vocabulary_id": null
171
      "vocabulary_id": null
            
165
    },
172
    },
            
166
    {
173
    {
            
167
      "display_name": "Coast and continental shelf",
174
      "display_name": "Coast and continental shelf",
            
168
      "id": "58eb1929-34b5-4fd9-ac44-824f0e51de40",
175
      "id": "58eb1929-34b5-4fd9-ac44-824f0e51de40",
            
169
      "name": "Coast and continental shelf",
176
      "name": "Coast and continental shelf",
            
170
      "state": "active",
177
      "state": "active",
            
171
      "vocabulary_id": null
178
      "vocabulary_id": null
            
172
    },
179
    },
            
173
    {
180
    {
            
174
      "display_name": "Gene expression incl. proteomics",
181
      "display_name": "Gene expression incl. proteomics",
            
175
      "id": "a266e76f-1f2a-427a-97e6-8e248d591f17",
182
      "id": "a266e76f-1f2a-427a-97e6-8e248d591f17",
            
176
      "name": "Gene expression incl. proteomics",
183
      "name": "Gene expression incl. proteomics",
            
177
      "state": "active",
184
      "state": "active",
            
178
      "vocabulary_id": null
185
      "vocabulary_id": null
            
179
    },
186
    },
            
180
    {
187
    {
            
181
      "display_name": "Hyas araneus",
188
      "display_name": "Hyas araneus",
            
182
      "id": "d0058ac3-4119-4d6d-b26f-d67fdc0ad12c",
189
      "id": "d0058ac3-4119-4d6d-b26f-d67fdc0ad12c",
            
183
      "name": "Hyas araneus",
190
      "name": "Hyas araneus",
            
184
      "state": "active",
191
      "state": "active",
            
185
      "vocabulary_id": null
192
      "vocabulary_id": null
            
186
    },
193
    },
            
187
    {
194
    {
            
188
      "display_name": "Laboratory experiment",
195
      "display_name": "Laboratory experiment",
            
189
      "id": "c6c00754-c769-4932-a517-bff2c9512884",
196
      "id": "c6c00754-c769-4932-a517-bff2c9512884",
            
190
      "name": "Laboratory experiment",
197
      "name": "Laboratory experiment",
            
191
      "state": "active",
198
      "state": "active",
            
192
      "vocabulary_id": null
199
      "vocabulary_id": null
            
193
    },
200
    },
            
194
    {
201
    {
            
n
195
      "display_name": "Mortality-Survival",
n
202
      "display_name": "Mortality",
            
196
      "id": "b2f19921-42bc-411a-9ae3-bbc203562a04",
203
      "id": "35c7bb04-7b62-4836-b299-63cd1060eece",
            
197
      "name": "Mortality-Survival",
204
      "name": "Mortality",
            
198
      "state": "active",
205
      "state": "active",
            
199
      "vocabulary_id": null
206
      "vocabulary_id": null
            
200
    },
207
    },
            
201
    {
208
    {
            
202
      "display_name": "North Atlantic",
209
      "display_name": "North Atlantic",
            
203
      "id": "72c150cb-b0a4-479b-a0c5-f23ebb039ab7",
210
      "id": "72c150cb-b0a4-479b-a0c5-f23ebb039ab7",
            
204
      "name": "North Atlantic",
211
      "name": "North Atlantic",
            
205
      "state": "active",
212
      "state": "active",
            
206
      "vocabulary_id": null
213
      "vocabulary_id": null
            
207
    },
214
    },
            
208
    {
215
    {
            
209
      "display_name": "Pelagos",
216
      "display_name": "Pelagos",
            
210
      "id": "012f5b69-4e75-4f54-8ac4-8bf192cafaa2",
217
      "id": "012f5b69-4e75-4f54-8ac4-8bf192cafaa2",
            
211
      "name": "Pelagos",
218
      "name": "Pelagos",
            
212
      "state": "active",
219
      "state": "active",
            
213
      "vocabulary_id": null
220
      "vocabulary_id": null
            
214
    },
221
    },
            
215
    {
222
    {
            
216
      "display_name": "Respiration",
223
      "display_name": "Respiration",
            
217
      "id": "494c54c9-c9ac-44d3-88fa-8e717d8113e0",
224
      "id": "494c54c9-c9ac-44d3-88fa-8e717d8113e0",
            
218
      "name": "Respiration",
225
      "name": "Respiration",
            
219
      "state": "active",
226
      "state": "active",
            
220
      "vocabulary_id": null
227
      "vocabulary_id": null
            
221
    },
228
    },
            
222
    {
229
    {
            
223
      "display_name": "Single species",
230
      "display_name": "Single species",
            
224
      "id": "88de69be-3bdd-4cf8-b6a1-386d72c5888d",
231
      "id": "88de69be-3bdd-4cf8-b6a1-386d72c5888d",
            
225
      "name": "Single species",
232
      "name": "Single species",
            
t
t
233
      "state": "active",
            
234
      "vocabulary_id": null
            
235
    },
            
236
    {
            
237
      "display_name": "Survival",
            
238
      "id": "85f42287-dcce-4d6f-b018-56f70887a7bc",
            
239
      "name": "Survival",
            
226
      "state": "active",
240
      "state": "active",
            
227
      "vocabulary_id": null
241
      "vocabulary_id": null
            
228
    },
242
    },
            
229
    {
243
    {
            
230
      "display_name": "Temperate",
244
      "display_name": "Temperate",
            
231
      "id": "31aaeb0c-a4a5-4a8e-9c90-be1eff0cac91",
245
      "id": "31aaeb0c-a4a5-4a8e-9c90-be1eff0cac91",
            
232
      "name": "Temperate",
246
      "name": "Temperate",
            
233
      "state": "active",
247
      "state": "active",
            
234
      "vocabulary_id": null
248
      "vocabulary_id": null
            
235
    },
249
    },
            
236
    {
250
    {
            
237
      "display_name": "Temperature",
251
      "display_name": "Temperature",
            
238
      "id": "8ffcba75-112d-48f4-b013-882017189fff",
252
      "id": "8ffcba75-112d-48f4-b013-882017189fff",
            
239
      "name": "Temperature",
253
      "name": "Temperature",
            
240
      "state": "active",
254
      "state": "active",
            
241
      "vocabulary_id": null
255
      "vocabulary_id": null
            
242
    },
256
    },
            
243
    {
257
    {
            
244
      "display_name": "Zooplankton",
258
      "display_name": "Zooplankton",
            
245
      "id": "9a829b96-807f-48c1-84c6-8fa50a64f5a5",
259
      "id": "9a829b96-807f-48c1-84c6-8fa50a64f5a5",
            
246
      "name": "Zooplankton",
260
      "name": "Zooplankton",
            
247
      "state": "active",
261
      "state": "active",
            
248
      "vocabulary_id": null
262
      "vocabulary_id": null
            
249
    }
263
    }
            
250
  ],
264
  ],
            
251
  "title": "Temperature tolerance of different larval stages of the 
265
  "title": "Temperature tolerance of different larval stages of the 
            
252
spider crab Hyas araneus exposed to elevated seawater PCO2",
266
spider crab Hyas araneus exposed to elevated seawater PCO2",
            
253
  "type": "vdataset",
267
  "type": "vdataset",
            
254
  "url": "https://doi.org/10.1594/PANGAEA.846762"
268
  "url": "https://doi.org/10.1594/PANGAEA.846762"
            
255
}
269
}