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  "author": "Brennan, Reid S",
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  "author": "Brennan, Reid S",
            
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  "author_email": "",
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  "author_email": "",
            
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  "citation": [],
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  "citation": [],
            
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  "creator_user_id": "17755db4-395a-4b3b-ac09-e8e3484ca700",
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  "creator_user_id": "17755db4-395a-4b3b-ac09-e8e3484ca700",
            
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  "doi": "10.1594/PANGAEA.912484",
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  "doi": "10.1594/PANGAEA.912484",
            
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  "doi_date_published": "2019",
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  "doi_date_published": "2019",
            
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  "doi_publisher": "",
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  "doi_publisher": "",
            
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  "doi_status": "True",
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  "doi_status": "True",
            
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  "extra_authors": [
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  "extra_authors": [
            
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    {
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    {
            
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      "extra_author": "Garrett, April D",
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      "extra_author": "Garrett, April D",
            
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      "familyName": "Garrett",
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      "familyName": "Garrett",
            
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      "givenName": "April D",
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      "givenName": "April D",
            
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      "orcid": ""
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      "orcid": ""
            
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    },
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    },
            
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    {
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    {
            
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      "extra_author": "Huber, Kaitlin E",
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      "extra_author": "Huber, Kaitlin E",
            
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      "familyName": "Huber",
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      "familyName": "Huber",
            
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      "givenName": "Kaitlin E",
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      "givenName": "Kaitlin E",
            
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      "orcid": ""
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      "orcid": ""
            
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    },
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    },
            
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    {
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    {
            
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      "extra_author": "Hargarten, Heidi",
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      "extra_author": "Hargarten, Heidi",
            
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      "familyName": "Hargarten",
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      "familyName": "Hargarten",
            
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      "givenName": "Heidi",
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      "givenName": "Heidi",
            
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      "orcid": "0000-0001-9278-1323"
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      "orcid": "0000-0001-9278-1323"
            
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    },
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    },
            
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    {
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    {
            
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      "extra_author": "Pespeni, Melissa H",
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      "extra_author": "Pespeni, Melissa H",
            
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      "familyName": "Pespeni",
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      "familyName": "Pespeni",
            
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      "givenName": "Melissa H",
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      "givenName": "Melissa H",
            
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      "orcid": "0000-0001-5447-6678"
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      "orcid": "0000-0001-5447-6678"
            
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    }
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    }
            
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  ],
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  ],
            
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  "familyName": "Brennan",
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  "familyName": "Brennan",
            
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  "givenName": "Reid S",
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  "givenName": "Reid S",
            
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  "groups": [],
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  "groups": [],
            
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  "id": "72d87aee-b233-45d4-a266-8215994680e6",
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  "license_id": "CC-BY-4.0",
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  "license_id": "CC-BY-4.0",
            
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  "license_title": "CC-BY-4.0",
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  "license_title": "CC-BY-4.0",
            
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  "metadata_created": "2024-11-29T11:46:21.133565",
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  "metadata_created": "2024-11-29T11:46:21.133565",
            
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  "metadata_modified": "2024-11-29T11:46:21.133572",
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  "metadata_modified": "2024-11-30T10:45:14.426596",
            
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  "name": "png-doi-10-1594-pangaea-912484",
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  "name": "png-doi-10-1594-pangaea-912484",
            
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  "notes": "Standing genetic variation is important for population 
46
  "notes": "Standing genetic variation is important for population 
            
47
persistence in extreme environmental conditions. While some species 
47
persistence in extreme environmental conditions. While some species 
            
48
may have the capacity to adapt to predicted average future global 
48
may have the capacity to adapt to predicted average future global 
            
49
change conditions, the ability to survive extreme events is largely 
49
change conditions, the ability to survive extreme events is largely 
            
50
unknown. We used single-generation selection experiments on hundreds 
50
unknown. We used single-generation selection experiments on hundreds 
            
51
of thousands of Strongylocentrotus purpuratus sea urchin larvae 
51
of thousands of Strongylocentrotus purpuratus sea urchin larvae 
            
52
generated from wild-caught adults to identify adaptive genetic 
52
generated from wild-caught adults to identify adaptive genetic 
            
53
variation responsive to moderate (pH 8.0) and extreme (pH 7.5) low-pH 
53
variation responsive to moderate (pH 8.0) and extreme (pH 7.5) low-pH 
            
54
conditions. Sequencing genomic DNA from pools of larvae, we identified 
54
conditions. Sequencing genomic DNA from pools of larvae, we identified 
            
55
consistent changes in allele frequencies across replicate cultures for 
55
consistent changes in allele frequencies across replicate cultures for 
            
56
each pH condition and observed increased linkage disequilibrium around 
56
each pH condition and observed increased linkage disequilibrium around 
            
57
selected loci, revealing selection on recombined standing genetic 
57
selected loci, revealing selection on recombined standing genetic 
            
58
variation. We found that loci responding uniquely to either selection 
58
variation. We found that loci responding uniquely to either selection 
            
59
regime were at low starting allele frequencies while variants that 
59
regime were at low starting allele frequencies while variants that 
            
60
responded to both pH conditions (11.6% of selected variants) started 
60
responded to both pH conditions (11.6% of selected variants) started 
            
61
at high frequencies. Loci under selection performed functions related 
61
at high frequencies. Loci under selection performed functions related 
            
62
to energetics, pH tolerance, cell growth and actin/cytoskeleton 
62
to energetics, pH tolerance, cell growth and actin/cytoskeleton 
            
63
dynamics. These results highlight that persistence in future 
63
dynamics. These results highlight that persistence in future 
            
64
conditions will require two classes of genetic variation: common, 
64
conditions will require two classes of genetic variation: common, 
            
65
pH-responsive variants maintained by balancing selection in a 
65
pH-responsive variants maintained by balancing selection in a 
            
66
heterogeneous environment, and rare variants, particularly for extreme 
66
heterogeneous environment, and rare variants, particularly for extreme 
            
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conditions, that must be maintained by large population sizes.",
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conditions, that must be maintained by large population sizes.",
            
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  "orcid": "0000-0001-7678-564X",
            
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    "approval_status": "approved",
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    "approval_status": "approved",
            
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    "created": "2024-11-29T11:32:00.143130",
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    "created": "2024-11-30T10:25:26.439072",
            
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    "description": "PANGAEA (Data Publisher for Earth & Environmental 
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    "description": "PANGAEA (Data Publisher for Earth & Environmental 
            
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Science): The information system PANGAEA is operated as an Open Access 
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Science): The information system PANGAEA is operated as an Open Access 
            
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library aimed at archiving, publishing and distributing georeferenced 
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library aimed at archiving, publishing and distributing georeferenced 
            
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data from earth system research. PANGAEA guarantees long-term 
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data from earth system research. PANGAEA guarantees long-term 
            
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availability (greater than 10 years) of its content. PANGAEA is open 
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availability (greater than 10 years) of its content. PANGAEA is open 
            
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to any project, institution, or individual scientist to use or to 
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to any project, institution, or individual scientist to use or to 
            
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archive and publish data. PANGAEA focuses on georeferenced 
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archive and publish data. PANGAEA focuses on georeferenced 
            
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observational data, experimental data, and models/simulations. 
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observational data, experimental data, and models/simulations. 
            
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Citability, comprehensive metadata descriptions, interoperability of 
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Citability, comprehensive metadata descriptions, interoperability of 
            
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data and metadata, a high degree of structural and semantic 
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data and metadata, a high degree of structural and semantic 
            
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harmonization of the data inventory as well as the commitment of the 
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harmonization of the data inventory as well as the commitment of the 
            
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hosting institutions ensures FAIRness of archived data.",
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hosting institutions ensures FAIRness of archived data.",
            
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    "id": "c2816fc6-b5ce-42c9-b5a4-11709b658b82",
            
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    "image_url": "pangaea_topicbiosphere.png",
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    "image_url": "pangaea_topicecology.png",
            
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    "is_organization": true,
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    "is_organization": true,
            
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    "name": "pangaea_biosphere",
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    "name": "pangaea_ecology",
            
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    "state": "active",
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    "state": "active",
            
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    "title": "PANGAEA (Biosphere)",
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    "title": "PANGAEA (Ecology)",
            
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    "type": "organization"
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    "type": "organization"
            
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  },
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  },
            
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  "owner_org": "3226ef9c-20d1-43fd-ba8f-fa35c8c9fb5d",
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  "private": false,
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  "private": false,
            
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  "publication_year": "2019",
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  "publication_year": "2019",
            
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  "related_identifiers": [
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  "related_identifiers": [
            
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    {
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    {
            
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      "authors": "Brennan Reid S,Garrett April D,Huber Kaitlin 
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      "authors": "Brennan Reid S,Garrett April D,Huber Kaitlin 
            
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E,Hargarten Heidi,Pespeni Melissa H",
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E,Hargarten Heidi,Pespeni Melissa H",
            
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      "email_authors": "rbrennan@geomar.de,,,,mpespeni@uvm.edu",
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      "email_authors": "rbrennan@geomar.de,,,,mpespeni@uvm.edu",
            
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      "identifier": "https://doi.org/10.1098/rspb.2019.0943",
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      "identifier": "https://doi.org/10.1098/rspb.2019.0943",
            
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      "identifier_type": "DOI",
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      "identifier_type": "DOI",
            
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      "orcid_authors": 
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      "orcid_authors": 
            
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"0000-0001-7678-564X,,,0000-0001-9278-1323,0000-0001-5447-6678",
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"0000-0001-7678-564X,,,0000-0001-9278-1323,0000-0001-5447-6678",
            
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      "relation_type": "IsSupplementTo",
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      "relation_type": "IsSupplementTo",
            
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      "source": "Proceedings of the Royal Society B-Biological 
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      "source": "Proceedings of the Royal Society B-Biological 
            
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Sciences",
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Sciences",
            
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      "title": "Rare genetic variation and balanced polymorphisms are 
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      "title": "Rare genetic variation and balanced polymorphisms are 
            
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important for survival in global change conditions",
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important for survival in global change conditions",
            
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      "year": "2019"
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      "year": "2019"
            
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    },
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    },
            
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    {
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    {
            
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      "authors": "Gattuso Jean-Pierre,Epitalon Jean-Marie,Lavigne 
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      "authors": "Gattuso Jean-Pierre,Epitalon Jean-Marie,Lavigne 
            
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H\u00e9lo\u00efse,Orr James C,Gentili Bernard,Hagens Mathilde,Hofmann 
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H\u00e9lo\u00efse,Orr James C,Gentili Bernard,Hagens Mathilde,Hofmann 
            
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Andreas,Mueller Jens-Daniel,Proye Aur\u00e9lien,Rae James,Soetaert 
116
Andreas,Mueller Jens-Daniel,Proye Aur\u00e9lien,Rae James,Soetaert 
            
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Karline",
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Karline",
            
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      "email_authors": 
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      "email_authors": 
            
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orr@iaea.org,bernard.gentili@orange.fr,,,,,,karline.soetaert@nioz.nl",
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orr@iaea.org,bernard.gentili@orange.fr,,,,,,karline.soetaert@nioz.nl",
            
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      "identifier": "https://CRAN.R-project.org/package=seacarb",
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      "identifier": "https://CRAN.R-project.org/package=seacarb",
            
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      "identifier_type": "DOI",
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      "identifier_type": "DOI",
            
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      "orcid_authors": 
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      "orcid_authors": 
            
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7080,,0000-0003-3980-1043,,,,0000-0003-3904-2526,0000-0003-4603-7100",
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7080,,0000-0003-3980-1043,,,,0000-0003-3904-2526,0000-0003-4603-7100",
            
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      "relation_type": "References",
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      "relation_type": "References",
            
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      "source": "",
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      "source": "",
            
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      "title": "seacarb: seawater carbonate chemistry with R. R 
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      "title": "seacarb: seawater carbonate chemistry with R. R 
            
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package version 3.2.12",
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package version 3.2.12",
            
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      "year": "2019"
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      "year": "2019"
            
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    }
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    }
            
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  ],
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  ],
            
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  "relationships_as_object": [],
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  "relationships_as_object": [],
            
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  "repository_name": "PANGAEA (Data Publisher for Earth & 
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  "repository_name": "PANGAEA (Data Publisher for Earth & 
            
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Environmental Science)",
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Environmental Science)",
            
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  "resource_type": "text/tab-separated-values - filename: 
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  "resource_type": "text/tab-separated-values - filename: 
            
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Brennan-etal_2019_PRSB",
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Brennan-etal_2019_PRSB",
            
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  "resources": [],
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  "resources": [],
            
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  "source_metadata_created": "2019",
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  "source_metadata_created": "2019",
            
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  "source_metadata_modified": "",
            
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  "state": "active",
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  "state": "active",
            
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    {
            
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      "subject_area_additional": "",
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      "subject_area_additional": "",
            
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      "subject_area_name": "BiologicalClassification"
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      "subject_area_name": "BiologicalClassification"
            
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    },
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    },
            
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    {
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    {
            
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      "subject_area_additional": "",
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      "subject_area_additional": "",
            
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      "subject_area_name": "Biosphere"
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      "subject_area_name": "Biosphere"
            
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    },
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    },
            
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    {
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    {
            
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      "subject_area_additional": "",
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      "subject_area_additional": "",
            
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      "subject_area_name": "Chemistry"
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      "subject_area_name": "Chemistry"
            
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    },
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    },
            
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    {
            
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      "subject_area_additional": "",
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      "subject_area_additional": "",
            
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      "subject_area_name": "Ecology"
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      "subject_area_name": "Ecology"
            
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    }
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    }
            
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  ],
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  "tags": [
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  "tags": [
            
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    {
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    {
            
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      "display_name": "Animalia",
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      "display_name": "Animalia",
            
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      "id": "e645e194-a2f7-40f1-ae7c-c1496be6f6bf",
            
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      "name": "Animalia",
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    {
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      "id": "c10fba87-d779-4e8c-8a1d-44ede960d1f9",
            
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      "name": "Echinodermata",
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      "name": "Echinodermata",
            
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      "state": "active",
            
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    {
            
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      "display_name": "Gene expression incl. proteomics",
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      "display_name": "Gene expression incl. proteomics",
            
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      "id": "a266e76f-1f2a-427a-97e6-8e248d591f17",
            
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      "name": "Gene expression incl. proteomics",
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      "name": "Gene expression incl. proteomics",
            
192
      "state": "active",
199
      "state": "active",
            
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      "vocabulary_id": null
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      "vocabulary_id": null
            
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    },
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    },
            
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    {
202
    {
            
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      "display_name": "Laboratory experiment",
203
      "display_name": "Laboratory experiment",
            
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      "id": "c6c00754-c769-4932-a517-bff2c9512884",
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      "id": "c6c00754-c769-4932-a517-bff2c9512884",
            
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      "name": "Laboratory experiment",
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      "name": "Laboratory experiment",
            
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      "state": "active",
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      "state": "active",
            
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      "vocabulary_id": null
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      "vocabulary_id": null
            
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    },
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    },
            
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    {
209
    {
            
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      "display_name": "North Pacific",
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      "display_name": "North Pacific",
            
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      "id": "efbdbaff-aa72-4a57-8e03-e9f84253d644",
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      "id": "efbdbaff-aa72-4a57-8e03-e9f84253d644",
            
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      "name": "North Pacific",
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      "name": "North Pacific",
            
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      "state": "active",
213
      "state": "active",
            
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      "vocabulary_id": null
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      "vocabulary_id": null
            
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    },
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    },
            
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    {
216
    {
            
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      "display_name": "Pelagos",
217
      "display_name": "Pelagos",
            
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      "id": "012f5b69-4e75-4f54-8ac4-8bf192cafaa2",
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      "id": "012f5b69-4e75-4f54-8ac4-8bf192cafaa2",
            
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      "name": "Pelagos",
219
      "name": "Pelagos",
            
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      "state": "active",
220
      "state": "active",
            
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      "vocabulary_id": null
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      "vocabulary_id": null
            
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    },
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    },
            
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    {
223
    {
            
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      "display_name": "Single species",
224
      "display_name": "Single species",
            
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      "id": "88de69be-3bdd-4cf8-b6a1-386d72c5888d",
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      "id": "88de69be-3bdd-4cf8-b6a1-386d72c5888d",
            
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      "name": "Single species",
226
      "name": "Single species",
            
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      "state": "active",
227
      "state": "active",
            
221
      "vocabulary_id": null
228
      "vocabulary_id": null
            
222
    },
229
    },
            
223
    {
230
    {
            
224
      "display_name": "Strongylocentrotus purpuratus",
231
      "display_name": "Strongylocentrotus purpuratus",
            
225
      "id": "a09abc6d-cf20-4baa-9b1a-d4f06aba8351",
232
      "id": "a09abc6d-cf20-4baa-9b1a-d4f06aba8351",
            
226
      "name": "Strongylocentrotus purpuratus",
233
      "name": "Strongylocentrotus purpuratus",
            
227
      "state": "active",
234
      "state": "active",
            
228
      "vocabulary_id": null
235
      "vocabulary_id": null
            
229
    },
236
    },
            
230
    {
237
    {
            
231
      "display_name": "Temperate",
238
      "display_name": "Temperate",
            
232
      "id": "31aaeb0c-a4a5-4a8e-9c90-be1eff0cac91",
239
      "id": "31aaeb0c-a4a5-4a8e-9c90-be1eff0cac91",
            
233
      "name": "Temperate",
240
      "name": "Temperate",
            
234
      "state": "active",
241
      "state": "active",
            
235
      "vocabulary_id": null
242
      "vocabulary_id": null
            
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    },
243
    },
            
237
    {
244
    {
            
238
      "display_name": "Zooplankton",
245
      "display_name": "Zooplankton",
            
239
      "id": "9a829b96-807f-48c1-84c6-8fa50a64f5a5",
246
      "id": "9a829b96-807f-48c1-84c6-8fa50a64f5a5",
            
240
      "name": "Zooplankton",
247
      "name": "Zooplankton",
            
241
      "state": "active",
248
      "state": "active",
            
242
      "vocabulary_id": null
249
      "vocabulary_id": null
            
243
    }
250
    }
            
244
  ],
251
  ],
            
245
  "title": "Seawater carbonate chemistry and body size, change in 
252
  "title": "Seawater carbonate chemistry and body size, change in 
            
246
allele frequency of Strongylocentrotus purpuratus larvae",
253
allele frequency of Strongylocentrotus purpuratus larvae",
            
247
  "type": "vdataset",
254
  "type": "vdataset",
            
248
  "url": "https://doi.org/10.1594/PANGAEA.912484"
255
  "url": "https://doi.org/10.1594/PANGAEA.912484"
            
249
}
256
}