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  "author": "Gibbin, Emma M",
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  "author": "Gibbin, Emma M",
            
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  "author_email": "",
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  "author_email": "",
            
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  "citation": [],
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  "citation": [],
            
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  "creator_user_id": "17755db4-395a-4b3b-ac09-e8e3484ca700",
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  "creator_user_id": "17755db4-395a-4b3b-ac09-e8e3484ca700",
            
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  "doi": "10.1594/PANGAEA.837880",
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  "doi": "10.1594/PANGAEA.837880",
            
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  "doi_date_published": "2014",
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  "doi_date_published": "2014",
            
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  "doi_publisher": "",
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  "doi_publisher": "",
            
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  "doi_status": "True",
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  "doi_status": "True",
            
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  "extra_authors": [
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  "extra_authors": [
            
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    {
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    {
            
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      "extra_author": "Putnam, H M",
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      "extra_author": "Putnam, H M",
            
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      "familyName": "Putnam",
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      "familyName": "Putnam",
            
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      "givenName": "H M",
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      "givenName": "H M",
            
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      "orcid": "0000-0003-2322-3269"
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      "orcid": "0000-0003-2322-3269"
            
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    },
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    },
            
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    {
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    {
            
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      "extra_author": "Davy, Simon K",
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      "extra_author": "Davy, Simon K",
            
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      "familyName": "Davy",
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      "familyName": "Davy",
            
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      "givenName": "Simon K",
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      "givenName": "Simon K",
            
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      "orcid": "0000-0003-3584-5356"
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      "orcid": "0000-0003-3584-5356"
            
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    },
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    },
            
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    {
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    {
            
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      "extra_author": "Gates, Ruth D",
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      "extra_author": "Gates, Ruth D",
            
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      "familyName": "Gates",
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      "familyName": "Gates",
            
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      "givenName": "Ruth D",
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      "givenName": "Ruth D",
            
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      "orcid": ""
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      "orcid": ""
            
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    }
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    }
            
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  ],
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  ],
            
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  "familyName": "Gibbin",
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  "familyName": "Gibbin",
            
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  "givenName": "Emma M",
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  "givenName": "Emma M",
            
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  "groups": [],
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  "groups": [],
            
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  "id": "58ef2e71-5b75-4cc1-84c5-7bc0e7bacdc7",
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  "id": "58ef2e71-5b75-4cc1-84c5-7bc0e7bacdc7",
            
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  "license_id": "CC-BY-3.0",
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  "license_id": "CC-BY-3.0",
            
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  "license_title": "CC-BY-3.0",
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  "license_title": "CC-BY-3.0",
            
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  "metadata_created": "2024-11-29T11:45:16.924572",
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  "metadata_created": "2024-11-29T11:45:16.924572",
            
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  "metadata_modified": "2024-11-29T11:45:16.924577",
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  "metadata_modified": "2024-11-30T14:30:05.945260",
            
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  "name": "png-doi-10-1594-pangaea-837880",
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  "name": "png-doi-10-1594-pangaea-837880",
            
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  "notes": "Regulating intracellular pH (pHi) is critical for 
40
  "notes": "Regulating intracellular pH (pHi) is critical for 
            
41
optimising the metabolic activity of corals, yet mechanisms involved 
41
optimising the metabolic activity of corals, yet mechanisms involved 
            
42
in pH regulation and the buffering capacity within coral cells are not 
42
in pH regulation and the buffering capacity within coral cells are not 
            
43
well understood. Our study investigated how the presence of symbiotic 
43
well understood. Our study investigated how the presence of symbiotic 
            
44
dinoflagellates affects the response of pHi to pCO2-driven seawater 
44
dinoflagellates affects the response of pHi to pCO2-driven seawater 
            
45
acidification in cells isolated from Pocillopora damicornis. Using the 
45
acidification in cells isolated from Pocillopora damicornis. Using the 
            
46
fluorescent dye BCECF-AM, in conjunction with confocal microscopy, we 
46
fluorescent dye BCECF-AM, in conjunction with confocal microscopy, we 
            
47
simultaneously characterised the response of pHi in host coral cells 
47
simultaneously characterised the response of pHi in host coral cells 
            
48
and their dinoflagellate symbionts, in symbiotic and non-symbiotic 
48
and their dinoflagellate symbionts, in symbiotic and non-symbiotic 
            
49
states under saturating light, with and without the photosynthetic 
49
states under saturating light, with and without the photosynthetic 
            
50
inhibitor DCMU. Each treatment was run under control (pH 7.8) and CO2 
50
inhibitor DCMU. Each treatment was run under control (pH 7.8) and CO2 
            
51
acidified seawater conditions (decreasing pH from 7.8 - 6.8). After 
51
acidified seawater conditions (decreasing pH from 7.8 - 6.8). After 
            
52
two hours of CO2 addition, by which time the external pH (pHe) had 
52
two hours of CO2 addition, by which time the external pH (pHe) had 
            
53
declined to 6.8, the dinoflagellate symbionts had increased their pHi 
53
declined to 6.8, the dinoflagellate symbionts had increased their pHi 
            
54
by 0.5 pH units above control levels. In contrast, in both symbiotic 
54
by 0.5 pH units above control levels. In contrast, in both symbiotic 
            
55
and non-symbiotic host coral cells, 15 min of CO2 addition (0.2 pH 
55
and non-symbiotic host coral cells, 15 min of CO2 addition (0.2 pH 
            
56
unit drop in pHe) led to cytoplasmic acidosis equivalent to 0.4 pH 
56
unit drop in pHe) led to cytoplasmic acidosis equivalent to 0.4 pH 
            
57
units. Despite further seawater acidification over the duration of the 
57
units. Despite further seawater acidification over the duration of the 
            
58
experiment, the pHi of non-symbiotic coral cells did not change, 
58
experiment, the pHi of non-symbiotic coral cells did not change, 
            
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though in host cells containing a symbiont cell the pHi recovered to 
59
though in host cells containing a symbiont cell the pHi recovered to 
            
60
control levels. This recovery was negated when cells were incubated 
60
control levels. This recovery was negated when cells were incubated 
            
61
with DCMU. Our results reveal that photosynthetic activity of the 
61
with DCMU. Our results reveal that photosynthetic activity of the 
            
62
endosymbiont is tightly coupled with the ability of the host cell to 
62
endosymbiont is tightly coupled with the ability of the host cell to 
            
63
recover from cellular acidosis after exposure to high CO2 / low pH.",
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recover from cellular acidosis after exposure to high CO2 / low pH.",
            
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    "approval_status": "approved",
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    "approval_status": "approved",
            
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    "created": "2024-11-29T11:32:00.143130",
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    "created": "2024-11-30T14:19:14.458899",
            
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    "description": "PANGAEA (Data Publisher for Earth & Environmental 
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    "description": "PANGAEA (Data Publisher for Earth & Environmental 
            
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Science): The information system PANGAEA is operated as an Open Access 
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Science): The information system PANGAEA is operated as an Open Access 
            
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library aimed at archiving, publishing and distributing georeferenced 
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library aimed at archiving, publishing and distributing georeferenced 
            
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data from earth system research. PANGAEA guarantees long-term 
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data from earth system research. PANGAEA guarantees long-term 
            
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availability (greater than 10 years) of its content. PANGAEA is open 
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availability (greater than 10 years) of its content. PANGAEA is open 
            
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to any project, institution, or individual scientist to use or to 
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to any project, institution, or individual scientist to use or to 
            
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archive and publish data. PANGAEA focuses on georeferenced 
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archive and publish data. PANGAEA focuses on georeferenced 
            
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observational data, experimental data, and models/simulations. 
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observational data, experimental data, and models/simulations. 
            
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Citability, comprehensive metadata descriptions, interoperability of 
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Citability, comprehensive metadata descriptions, interoperability of 
            
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data and metadata, a high degree of structural and semantic 
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data and metadata, a high degree of structural and semantic 
            
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harmonization of the data inventory as well as the commitment of the 
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harmonization of the data inventory as well as the commitment of the 
            
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hosting institutions ensures FAIRness of archived data.",
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hosting institutions ensures FAIRness of archived data.",
            
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    "image_url": "pangaea_topicbiosphere.png",
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    "image_url": "pangaea_topicbiologicalclassification.png",
            
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    "is_organization": true,
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    "is_organization": true,
            
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    "name": "pangaea_biosphere",
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    "name": "pangaea_biologicalclassification",
            
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    "state": "active",
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    "state": "active",
            
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    "title": "PANGAEA (Biosphere)",
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    "title": "PANGAEA (Biological Classification)",
            
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    "type": "organization"
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    "type": "organization"
            
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  },
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  "owner_org": "3226ef9c-20d1-43fd-ba8f-fa35c8c9fb5d",
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  "private": false,
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  "private": false,
            
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  "publication_year": "2014",
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  "publication_year": "2014",
            
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  "related_identifiers": [
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  "related_identifiers": [
            
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    {
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    {
            
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      "authors": "Gibbin Emma M,Putnam H M,Davy Simon K,Gates Ruth D",
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      "authors": "Gibbin Emma M,Putnam H M,Davy Simon K,Gates Ruth D",
            
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      "email_authors": 
96
      "email_authors": 
            
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"gibbin@hotmail.co.uk,hputnam@hawaii.edu,simon.davy@vuw.ac.nz,",
97
"gibbin@hotmail.co.uk,hputnam@hawaii.edu,simon.davy@vuw.ac.nz,",
            
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      "identifier": "https://doi.org/10.1242/jeb.099549",
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      "identifier": "https://doi.org/10.1242/jeb.099549",
            
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      "identifier_type": "DOI",
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      "identifier_type": "DOI",
            
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      "orcid_authors": ",0000-0003-2322-3269,0000-0003-3584-5356,",
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      "orcid_authors": ",0000-0003-2322-3269,0000-0003-3584-5356,",
            
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      "relation_type": "IsSupplementTo",
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      "relation_type": "IsSupplementTo",
            
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      "source": "Journal of Experimental Biology",
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      "source": "Journal of Experimental Biology",
            
103
      "title": "Intracellular pH and its response to CO2-driven 
103
      "title": "Intracellular pH and its response to CO2-driven 
            
104
seawater acidification in symbiotic versus non-symbiotic coral cells",
104
seawater acidification in symbiotic versus non-symbiotic coral cells",
            
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      "year": "2014"
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      "year": "2014"
            
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    },
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    },
            
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    {
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    {
            
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      "authors": "Lavigne H\u00e9lo\u00efse,Epitalon 
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      "authors": "Lavigne H\u00e9lo\u00efse,Epitalon 
            
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Jean-Marie,Gattuso Jean-Pierre",
109
Jean-Marie,Gattuso Jean-Pierre",
            
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      "email_authors": 
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      "email_authors": 
            
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"hlavigne@naturalsciences.be,,jean-pierre.gattuso@imev-mer.fr",
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"hlavigne@naturalsciences.be,,jean-pierre.gattuso@imev-mer.fr",
            
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      "identifier": "https://cran.r-project.org/package=seacarb",
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      "identifier": "https://cran.r-project.org/package=seacarb",
            
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      "identifier_type": "DOI",
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      "identifier_type": "DOI",
            
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      "orcid_authors": ",,0000-0002-4533-4114",
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      "orcid_authors": ",,0000-0002-4533-4114",
            
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      "relation_type": "References",
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      "relation_type": "References",
            
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      "source": "",
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      "source": "",
            
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      "title": "seacarb: seawater carbonate chemistry with R. R 
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      "title": "seacarb: seawater carbonate chemistry with R. R 
            
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package version 3.0",
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package version 3.0",
            
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      "year": "2014"
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      "year": "2014"
            
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    }
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    }
            
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  ],
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  ],
            
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  "relationships_as_object": [],
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  "relationships_as_subject": [],
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  "relationships_as_subject": [],
            
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  "repository_name": "PANGAEA (Data Publisher for Earth & 
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  "repository_name": "PANGAEA (Data Publisher for Earth & 
            
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Environmental Science)",
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Environmental Science)",
            
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  "resource_type": "text/tab-separated-values - filename: 
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  "resource_type": "text/tab-separated-values - filename: 
            
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Schluter_2014",
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Schluter_2014",
            
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  "resources": [],
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  "resources": [],
            
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  "source_metadata_created": "2014",
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  "source_metadata_created": "2014",
            
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  "source_metadata_modified": "",
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  "source_metadata_modified": "",
            
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  "state": "active",
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  "state": "active",
            
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    {
            
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      "subject_area_additional": "",
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      "subject_area_additional": "",
            
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      "subject_area_name": "BiologicalClassification"
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      "subject_area_name": "BiologicalClassification"
            
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    },
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    },
            
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    {
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    {
            
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      "subject_area_additional": "",
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      "subject_area_additional": "",
            
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      "subject_area_name": "Biosphere"
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      "subject_area_name": "Biosphere"
            
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    },
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    },
            
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    {
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    {
            
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      "subject_area_additional": "",
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      "subject_area_additional": "",
            
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      "subject_area_name": "Chemistry"
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      "subject_area_name": "Chemistry"
            
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    }
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    }
            
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  ],
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  ],
            
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  "tags": [
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  "tags": [
            
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      "display_name": "1000 L or  1 m2",
            
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      "id": "d920ecdb-c44e-43f2-bb4c-ffdc3a3c398c",
            
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      "name": "1000 L or  1 m2",
            
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      "display_name": "Acid-base regulation",
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      "state": "active",
            
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      "display_name": "Animalia",
            
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      "name": "Animalia",
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      "name": "Animalia",
            
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      "display_name": "Benthic animals",
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      "display_name": "Benthic animals",
            
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      "id": "ba711ac9-d2d3-4cff-8023-8b85163344bb",
            
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      "name": "Benthic animals",
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      "name": "Benthic animals",
            
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      "display_name": "Benthos",
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      "display_name": "Benthos",
            
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      "id": "55bc70f2-ff73-4d9a-89f6-16358d0d9b87",
            
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      "name": "Benthos",
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      "name": "Benthos",
            
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      "state": "active",
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      "state": "active",
            
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    },
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    },
            
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    {
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    {
            
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      "display_name": "Cnidaria",
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      "display_name": "Cnidaria",
            
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      "id": "5c3a6df6-61df-4230-b9e3-7768cbada71d",
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      "id": "5c3a6df6-61df-4230-b9e3-7768cbada71d",
            
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      "name": "Cnidaria",
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      "name": "Cnidaria",
            
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      "state": "active",
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      "state": "active",
            
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      "vocabulary_id": null
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      "vocabulary_id": null
            
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    },
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    },
            
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    {
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    {
            
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      "display_name": "Coast and continental shelf",
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      "display_name": "Coast and continental shelf",
            
184
      "id": "58eb1929-34b5-4fd9-ac44-824f0e51de40",
191
      "id": "58eb1929-34b5-4fd9-ac44-824f0e51de40",
            
185
      "name": "Coast and continental shelf",
192
      "name": "Coast and continental shelf",
            
186
      "state": "active",
193
      "state": "active",
            
187
      "vocabulary_id": null
194
      "vocabulary_id": null
            
188
    },
195
    },
            
189
    {
196
    {
            
n
190
      "display_name": "Containers and aquaria 20-1000 L or  1 m2",
n
197
      "display_name": "Containers and aquaria 20",
            
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      "id": "a9d9036d-3fe8-4fb1-bcb9-07b8a3a3fe11",
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      "id": "18d57eca-7aec-4d8a-9a57-92bf32a45d53",
            
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      "name": "Containers and aquaria 20-1000 L or  1 m2",
199
      "name": "Containers and aquaria 20",
            
193
      "state": "active",
200
      "state": "active",
            
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      "vocabulary_id": null
201
      "vocabulary_id": null
            
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    },
202
    },
            
196
    {
203
    {
            
197
      "display_name": "Laboratory experiment",
204
      "display_name": "Laboratory experiment",
            
198
      "id": "c6c00754-c769-4932-a517-bff2c9512884",
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      "id": "c6c00754-c769-4932-a517-bff2c9512884",
            
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      "name": "Laboratory experiment",
206
      "name": "Laboratory experiment",
            
200
      "state": "active",
207
      "state": "active",
            
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      "vocabulary_id": null
208
      "vocabulary_id": null
            
202
    },
209
    },
            
203
    {
210
    {
            
204
      "display_name": "North Pacific",
211
      "display_name": "North Pacific",
            
205
      "id": "efbdbaff-aa72-4a57-8e03-e9f84253d644",
212
      "id": "efbdbaff-aa72-4a57-8e03-e9f84253d644",
            
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      "name": "North Pacific",
213
      "name": "North Pacific",
            
207
      "state": "active",
214
      "state": "active",
            
208
      "vocabulary_id": null
215
      "vocabulary_id": null
            
209
    },
216
    },
            
210
    {
217
    {
            
211
      "display_name": "Pocillopora damicornis",
218
      "display_name": "Pocillopora damicornis",
            
212
      "id": "a4eed90a-849f-49bb-b5c9-a2e3516a614c",
219
      "id": "a4eed90a-849f-49bb-b5c9-a2e3516a614c",
            
213
      "name": "Pocillopora damicornis",
220
      "name": "Pocillopora damicornis",
            
214
      "state": "active",
221
      "state": "active",
            
215
      "vocabulary_id": null
222
      "vocabulary_id": null
            
216
    },
223
    },
            
217
    {
224
    {
            
218
      "display_name": "Single species",
225
      "display_name": "Single species",
            
219
      "id": "88de69be-3bdd-4cf8-b6a1-386d72c5888d",
226
      "id": "88de69be-3bdd-4cf8-b6a1-386d72c5888d",
            
220
      "name": "Single species",
227
      "name": "Single species",
            
221
      "state": "active",
228
      "state": "active",
            
222
      "vocabulary_id": null
229
      "vocabulary_id": null
            
223
    },
230
    },
            
224
    {
231
    {
            
225
      "display_name": "Tropical",
232
      "display_name": "Tropical",
            
226
      "id": "db8bd80f-8578-455b-acc6-6adbeae406b8",
233
      "id": "db8bd80f-8578-455b-acc6-6adbeae406b8",
            
227
      "name": "Tropical",
234
      "name": "Tropical",
            
t
t
235
      "state": "active",
            
236
      "vocabulary_id": null
            
237
    },
            
238
    {
            
239
      "display_name": "base regulation",
            
240
      "id": "7e6acb21-37f2-4d0b-89e9-0ad04a1db8aa",
            
241
      "name": "base regulation",
            
228
      "state": "active",
242
      "state": "active",
            
229
      "vocabulary_id": null
243
      "vocabulary_id": null
            
230
    }
244
    }
            
231
  ],
245
  ],
            
232
  "title": "Adaptation of a globally important coccolithophore to 
246
  "title": "Adaptation of a globally important coccolithophore to 
            
233
ocean warming and acidification",
247
ocean warming and acidification",
            
234
  "type": "vdataset",
248
  "type": "vdataset",
            
235
  "url": "https://doi.org/10.1594/PANGAEA.837880"
249
  "url": "https://doi.org/10.1594/PANGAEA.837880"
            
236
}
250
}