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f | 1 | { | f | 1 | { |
2 | "author": "Feiner, Alexander", | 2 | "author": "Feiner, Alexander", | ||
3 | "author_email": "", | 3 | "author_email": "", | ||
4 | "creator_user_id": "17755db4-395a-4b3b-ac09-e8e3484ca700", | 4 | "creator_user_id": "17755db4-395a-4b3b-ac09-e8e3484ca700", | ||
5 | "doi": "10.22000/319", | 5 | "doi": "10.22000/319", | ||
6 | "doi_date_published": "2020", | 6 | "doi_date_published": "2020", | ||
7 | "doi_publisher": "", | 7 | "doi_publisher": "", | ||
8 | "doi_status": "True", | 8 | "doi_status": "True", | ||
9 | "extra_authors": [ | 9 | "extra_authors": [ | ||
10 | { | 10 | { | ||
11 | "extra_author": "Pitra, Nicholi", | 11 | "extra_author": "Pitra, Nicholi", | ||
12 | "orcid": "0000-0001-8642-5138" | 12 | "orcid": "0000-0001-8642-5138" | ||
13 | }, | 13 | }, | ||
14 | { | 14 | { | ||
15 | "extra_author": "Matthews, Paul", | 15 | "extra_author": "Matthews, Paul", | ||
16 | "orcid": "" | 16 | "orcid": "" | ||
17 | }, | 17 | }, | ||
18 | { | 18 | { | ||
19 | "extra_author": "Pillen, Klaus", | 19 | "extra_author": "Pillen, Klaus", | ||
20 | "orcid": "" | 20 | "orcid": "" | ||
21 | }, | 21 | }, | ||
22 | { | 22 | { | ||
23 | "extra_author": "Wessjohann, Ludger A.", | 23 | "extra_author": "Wessjohann, Ludger A.", | ||
24 | "orcid": "0000-0003-2060-8235" | 24 | "orcid": "0000-0003-2060-8235" | ||
25 | }, | 25 | }, | ||
26 | { | 26 | { | ||
27 | "extra_author": "Riewe, David", | 27 | "extra_author": "Riewe, David", | ||
28 | "orcid": "0000-0002-9095-5518" | 28 | "orcid": "0000-0002-9095-5518" | ||
29 | } | 29 | } | ||
30 | ], | 30 | ], | ||
31 | "groups": [], | 31 | "groups": [], | ||
32 | "id": "aadf20e9-6251-440b-ac9d-550737e93140", | 32 | "id": "aadf20e9-6251-440b-ac9d-550737e93140", | ||
33 | "isopen": false, | 33 | "isopen": false, | ||
34 | "license_id": "CC BY 4.0 Attribution", | 34 | "license_id": "CC BY 4.0 Attribution", | ||
35 | "license_title": "CC BY 4.0 Attribution", | 35 | "license_title": "CC BY 4.0 Attribution", | ||
36 | "metadata_created": "2023-01-12T13:30:28.386448", | 36 | "metadata_created": "2023-01-12T13:30:28.386448", | ||
t | 37 | "metadata_modified": "2023-08-04T08:51:20.180596", | t | 37 | "metadata_modified": "2023-08-04T08:52:48.027427", |
38 | "name": "rdr-doi-10-22000-319", | 38 | "name": "rdr-doi-10-22000-319", | ||
39 | "notes": "Abstract: Downy mildew in hop (Humulus lupulus L.) is | 39 | "notes": "Abstract: Downy mildew in hop (Humulus lupulus L.) is | ||
40 | caused by Pseudoperonospora humuli and generates significant losses in | 40 | caused by Pseudoperonospora humuli and generates significant losses in | ||
41 | quality and yield. To identify the biochemical processes that confer | 41 | quality and yield. To identify the biochemical processes that confer | ||
42 | natural downy mildew resistance (DMR), a metabolome- and genome-wide | 42 | natural downy mildew resistance (DMR), a metabolome- and genome-wide | ||
43 | association study was performed. Inoculation of a high density | 43 | association study was performed. Inoculation of a high density | ||
44 | genotyped F1 hop population (n=192) with the obligate biotrophic | 44 | genotyped F1 hop population (n=192) with the obligate biotrophic | ||
45 | oomycete P. humuli led to variation in both the levels of thousands of | 45 | oomycete P. humuli led to variation in both the levels of thousands of | ||
46 | specialized metabolites and DMR. We observed that metabolites of | 46 | specialized metabolites and DMR. We observed that metabolites of | ||
47 | almost all major phytochemical classes were induced 48 hours after | 47 | almost all major phytochemical classes were induced 48 hours after | ||
48 | inoculation. But only a small number of metabolites were found to be | 48 | inoculation. But only a small number of metabolites were found to be | ||
49 | correlated with DMR and these were enriched with phenylpropanoids. | 49 | correlated with DMR and these were enriched with phenylpropanoids. | ||
50 | These metabolites were also correlated with DMR when measured from the | 50 | These metabolites were also correlated with DMR when measured from the | ||
51 | non-infected control set. A genome-wide association study revealed | 51 | non-infected control set. A genome-wide association study revealed | ||
52 | co-localization of the major DMR loci and the phenylpropanoid pathway | 52 | co-localization of the major DMR loci and the phenylpropanoid pathway | ||
53 | markers indicating that the major contribution to resistance is | 53 | markers indicating that the major contribution to resistance is | ||
54 | mediated by these metabolites in a heritable manner. The application | 54 | mediated by these metabolites in a heritable manner. The application | ||
55 | of three putative prophylactic phenylpropanoids led to a reduced | 55 | of three putative prophylactic phenylpropanoids led to a reduced | ||
56 | degree of leaf infection in susceptible genotypes, confirming their | 56 | degree of leaf infection in susceptible genotypes, confirming their | ||
57 | protective activity either directly or as precursors of active | 57 | protective activity either directly or as precursors of active | ||
58 | compounds.", | 58 | compounds.", | ||
59 | "num_resources": 0, | 59 | "num_resources": 0, | ||
60 | "num_tags": 6, | 60 | "num_tags": 6, | ||
61 | "orcid": "0000-0001-6564-8826", | 61 | "orcid": "0000-0001-6564-8826", | ||
62 | "organization": { | 62 | "organization": { | ||
63 | "approval_status": "approved", | 63 | "approval_status": "approved", | ||
64 | "created": "2023-01-12T13:30:23.238233", | 64 | "created": "2023-01-12T13:30:23.238233", | ||
65 | "description": "RADAR (Research Data Repository) is a | 65 | "description": "RADAR (Research Data Repository) is a | ||
66 | cross-disciplinary repository for archiving and publishing research | 66 | cross-disciplinary repository for archiving and publishing research | ||
67 | data from completed scientific studies and projects. The focus is on | 67 | data from completed scientific studies and projects. The focus is on | ||
68 | research data from subjects that do not yet have their own | 68 | research data from subjects that do not yet have their own | ||
69 | discipline-specific infrastructures for research data management. ", | 69 | discipline-specific infrastructures for research data management. ", | ||
70 | "id": "013c89a9-383c-4200-8baa-0f78bf1d91f9", | 70 | "id": "013c89a9-383c-4200-8baa-0f78bf1d91f9", | ||
71 | "image_url": "radar-logo.svg", | 71 | "image_url": "radar-logo.svg", | ||
72 | "is_organization": true, | 72 | "is_organization": true, | ||
73 | "name": "radar", | 73 | "name": "radar", | ||
74 | "state": "active", | 74 | "state": "active", | ||
75 | "title": "RADAR", | 75 | "title": "RADAR", | ||
76 | "type": "organization" | 76 | "type": "organization" | ||
77 | }, | 77 | }, | ||
78 | "owner_org": "013c89a9-383c-4200-8baa-0f78bf1d91f9", | 78 | "owner_org": "013c89a9-383c-4200-8baa-0f78bf1d91f9", | ||
79 | "private": false, | 79 | "private": false, | ||
80 | "production_year": "2015", | 80 | "production_year": "2015", | ||
81 | "publication_year": "2020", | 81 | "publication_year": "2020", | ||
82 | "publishers": [ | 82 | "publishers": [ | ||
83 | { | 83 | { | ||
84 | "publisher": "Simon H. Steiner, Hopfen, GmbH, Mainburg, Germany" | 84 | "publisher": "Simon H. Steiner, Hopfen, GmbH, Mainburg, Germany" | ||
85 | }, | 85 | }, | ||
86 | { | 86 | { | ||
87 | "publisher": "Leibniz Institute of Plant Genetics and Crop Plant | 87 | "publisher": "Leibniz Institute of Plant Genetics and Crop Plant | ||
88 | Research (IPK), Gatersleben, Germany" | 88 | Research (IPK), Gatersleben, Germany" | ||
89 | }, | 89 | }, | ||
90 | { | 90 | { | ||
91 | "publisher": "Julius Kuehn-Institute (JKI), Berlin, Germany" | 91 | "publisher": "Julius Kuehn-Institute (JKI), Berlin, Germany" | ||
92 | }, | 92 | }, | ||
93 | { | 93 | { | ||
94 | "publisher": "Leibniz Institute of Plant Biochemistry (IPB), | 94 | "publisher": "Leibniz Institute of Plant Biochemistry (IPB), | ||
95 | Halle (Saale), Germany" | 95 | Halle (Saale), Germany" | ||
96 | } | 96 | } | ||
97 | ], | 97 | ], | ||
98 | "relationships_as_object": [], | 98 | "relationships_as_object": [], | ||
99 | "relationships_as_subject": [], | 99 | "relationships_as_subject": [], | ||
100 | "repository_name": "RADAR (Research Data Repository)", | 100 | "repository_name": "RADAR (Research Data Repository)", | ||
101 | "resource_type": "Dataset - MS Peak table", | 101 | "resource_type": "Dataset - MS Peak table", | ||
102 | "resources": [], | 102 | "resources": [], | ||
103 | "services_used_list": "", | 103 | "services_used_list": "", | ||
104 | "source_metadata_created": "2020", | 104 | "source_metadata_created": "2020", | ||
105 | "source_metadata_modified": "", | 105 | "source_metadata_modified": "", | ||
106 | "state": "active", | 106 | "state": "active", | ||
107 | "subject_areas": [ | 107 | "subject_areas": [ | ||
108 | { | 108 | { | ||
109 | "subject_area_additional": "", | 109 | "subject_area_additional": "", | ||
110 | "subject_area_name": "Biochemistry" | 110 | "subject_area_name": "Biochemistry" | ||
111 | }, | 111 | }, | ||
112 | { | 112 | { | ||
113 | "subject_area_additional": "", | 113 | "subject_area_additional": "", | ||
114 | "subject_area_name": "Agriculture" | 114 | "subject_area_name": "Agriculture" | ||
115 | } | 115 | } | ||
116 | ], | 116 | ], | ||
117 | "tags": [ | 117 | "tags": [ | ||
118 | { | 118 | { | ||
119 | "display_name": "downy mildew resistance", | 119 | "display_name": "downy mildew resistance", | ||
120 | "id": "eb00d134-acff-407d-9361-307bcc40f1e7", | 120 | "id": "eb00d134-acff-407d-9361-307bcc40f1e7", | ||
121 | "name": "downy mildew resistance", | 121 | "name": "downy mildew resistance", | ||
122 | "state": "active", | 122 | "state": "active", | ||
123 | "vocabulary_id": null | 123 | "vocabulary_id": null | ||
124 | }, | 124 | }, | ||
125 | { | 125 | { | ||
126 | "display_name": "genome-wide association study", | 126 | "display_name": "genome-wide association study", | ||
127 | "id": "e0e5c774-d57a-435e-8418-e86330b7f7a0", | 127 | "id": "e0e5c774-d57a-435e-8418-e86330b7f7a0", | ||
128 | "name": "genome-wide association study", | 128 | "name": "genome-wide association study", | ||
129 | "state": "active", | 129 | "state": "active", | ||
130 | "vocabulary_id": null | 130 | "vocabulary_id": null | ||
131 | }, | 131 | }, | ||
132 | { | 132 | { | ||
133 | "display_name": "hop", | 133 | "display_name": "hop", | ||
134 | "id": "e2a218b7-02e0-460b-941e-69da131b30aa", | 134 | "id": "e2a218b7-02e0-460b-941e-69da131b30aa", | ||
135 | "name": "hop", | 135 | "name": "hop", | ||
136 | "state": "active", | 136 | "state": "active", | ||
137 | "vocabulary_id": null | 137 | "vocabulary_id": null | ||
138 | }, | 138 | }, | ||
139 | { | 139 | { | ||
140 | "display_name": "oomycete", | 140 | "display_name": "oomycete", | ||
141 | "id": "3a5b9fd3-a0ba-40e7-bf52-bb5d2421de10", | 141 | "id": "3a5b9fd3-a0ba-40e7-bf52-bb5d2421de10", | ||
142 | "name": "oomycete", | 142 | "name": "oomycete", | ||
143 | "state": "active", | 143 | "state": "active", | ||
144 | "vocabulary_id": null | 144 | "vocabulary_id": null | ||
145 | }, | 145 | }, | ||
146 | { | 146 | { | ||
147 | "display_name": "phenylpropanoids", | 147 | "display_name": "phenylpropanoids", | ||
148 | "id": "74603eb2-37cc-4f92-bfc8-a0dcb3630b1c", | 148 | "id": "74603eb2-37cc-4f92-bfc8-a0dcb3630b1c", | ||
149 | "name": "phenylpropanoids", | 149 | "name": "phenylpropanoids", | ||
150 | "state": "active", | 150 | "state": "active", | ||
151 | "vocabulary_id": null | 151 | "vocabulary_id": null | ||
152 | }, | 152 | }, | ||
153 | { | 153 | { | ||
154 | "display_name": "untargeted metabolomics", | 154 | "display_name": "untargeted metabolomics", | ||
155 | "id": "cfd280db-a29d-4fdb-bcf9-0f5d31e0f746", | 155 | "id": "cfd280db-a29d-4fdb-bcf9-0f5d31e0f746", | ||
156 | "name": "untargeted metabolomics", | 156 | "name": "untargeted metabolomics", | ||
157 | "state": "active", | 157 | "state": "active", | ||
158 | "vocabulary_id": null | 158 | "vocabulary_id": null | ||
159 | } | 159 | } | ||
160 | ], | 160 | ], | ||
161 | "title": "Supplementary data for \"downy mildew resistance is | 161 | "title": "Supplementary data for \"downy mildew resistance is | ||
162 | genetically mediated by prophylactic production of phenylpropanoids in | 162 | genetically mediated by prophylactic production of phenylpropanoids in | ||
163 | hop\"", | 163 | hop\"", | ||
164 | "type": "vdataset", | 164 | "type": "vdataset", | ||
165 | "url": "https://doi.org/10.22000/319" | 165 | "url": "https://doi.org/10.22000/319" | ||
166 | } | 166 | } |