Seawater carbonate chemistry and sperm-egg collisions, gamete fusion, and generation of Ca2+ oscillations of a broadcast spawning bivalve, Tegillarca granosa

doi:doi:10.1594/PANGAEA.888184

Seawater carbonate chemistry and sperm-egg collisions, gamete fusion, and generation of Ca2+ oscillations of a broadcast spawning bivalve, Tegillarca granosa

Although the effect of ocean acidification on fertilization success of marine organisms is increasingly well documented, the underlying mechanisms are not completely understood. The fertilization success of broadcast spawning invertebrates depends on successful sperm-egg collisions, gamete fusion, and standard generation of Ca2+oscillations. Therefore, the realistic effects of future ocean pCO2 levels on these specific aspects of fertilization of Tegillarca granosa were investigated in the present study through sperm velocity trials, fertilization kinetics model analysis, and intracellular Ca2+assays, respectively. Results obtained indicated that ocean acidification significantly reduced the fertilization success of T. granosa, which could be accountable by (i) decreased sperm velocity hence reducing the probability for sperm-egg collisions; (ii) lowered probability of gamete fusion for each gamete collision event; and (iii) disrupted intracellular Ca2+ oscillations.

BibTex:

@dataset{Shi_Wei_and_Han_Yu_and_Guo_Cheng_and_Zhao_Xinguo_and_Liu_Saixi_and_Su_Wenhao_and_Wang_Yichen_and_Zha_Shanjie_and_Chai_Xueliang_and_Liu_Guangxu_2017,
    abstract = {Although the effect of ocean acidification on fertilization success of marine organisms is increasingly well documented, the underlying mechanisms are not completely understood. The fertilization success of broadcast spawning invertebrates depends on successful sperm-egg collisions, gamete fusion, and standard generation of Ca2+oscillations. Therefore, the realistic effects of future ocean pCO2 levels on these specific aspects of fertilization of Tegillarca granosa were investigated in the present study through sperm velocity trials, fertilization kinetics model analysis, and intracellular Ca2+assays, respectively. Results obtained indicated that ocean acidification significantly reduced the fertilization success of T. granosa, which could be accountable by (i) decreased sperm velocity hence reducing the probability for sperm-egg collisions; (ii) lowered probability of gamete fusion for each gamete collision event; and (iii) disrupted intracellular Ca2+ oscillations.},
    author = {Shi, Wei and Han, Yu and Guo, Cheng and Zhao, Xinguo and Liu, Saixi and Su, Wenhao and Wang, Yichen and Zha, Shanjie and Chai, Xueliang and Liu, Guangxu},
    doi = {10.1594/PANGAEA.888184},
    institution = {PANGAEA (Ecology)},
    keyword = {'Animalia', 'Aquaria 20 L', 'Benthic animals', 'Benthos', 'Bottles or small containers', 'Coast and continental shelf', 'Laboratory experiment', 'Mollusca', 'North Pacific', 'Other metabolic rates', 'Reproduction', 'Single species', 'Tegillarca granosa', 'Temperate'},
    title = {Seawater carbonate chemistry and sperm-egg collisions, gamete fusion, and generation of Ca2+ oscillations of a broadcast spawning bivalve, Tegillarca granosa},
    url = {https://service.tib.eu/ldmservice/vdataset/png-doi-10-1594-pangaea-888184},
    year = {2017}
}