Disaggregated indurated carbonate samples using Calgon, acetic acid and electric pulse fragmentation

Three disaggregation methods, i.e. Calgon, acetic acid and electric pulse fragmentation (EPF), have been applied to a range of heavily lithified, carbonate-rich sedimentary rock samples of Paleogene age. Samples are predominantly from the carbonate-rich, shallow water domain ( 500 μm, suggesting this technique may be more appropriate for liberating larger microfossils (e.g. LFs); however, we discuss nuances to the method that would allow for more effective recovery of smaller microfossil specimens. The more traditional acetic acid method was also able to disaggregate a number of the samples; however, preservation of the LF was compromised. We suggest a best-practice methodology for implementing EPF in micropalaeontological studies.