Experiments on coexisting phytoplankton species Chaetoceros affinis and Emiliania huxleyi in response to nutrients

Measurements of cell size, cell density, nutrient concentration and genotype composition in a long-term experiment (182 days) with the marine phytoplankton species Chaetoceros affinis and Emiliania huxleyi, each consisting of nine genotypes. The species were cultivated together at three different nutrient regimes (10 N, 20 N, 30 N) with increasing nitrate supply in a semi-continuous batch cycle system. The genotype composition of both species was assessed after 49, 91, and 182 days using microsatellites. In a short-term experiment cell size and density of nine Chaetoceros affinis genotypes separately were measured after 7 days growth at seven nitrate levels (2.5, 5, 7.5, 12.5, 20, 30, and 45 μmol L−1 N).