Methane concentrations in deep-sea mussel culture: hand feed

During culture of deep-sea mussels Bathymodiolus azoricus in November 2013, the culture system (200 L) was repetitively enriched with methane by adding 5 L CH4-saturated sea water (after 15 minutes of equilibration with a gas diffusor stone, solution containing ~8 mmol of CH4). The CH4 concentrations were continuously measured by CONTROS HydroC® CH₄ sensor placed in the culture system.