Impact of climate change on the kelp Laminaria digitata: Biochemical and physiological raw data of a long-term experiment simulating Artic winter warming

doi:doi:10.1594/PANGAEA.972789

Impact of climate change on the kelp Laminaria digitata: Biochemical and physiological raw data of a long-term experiment simulating Artic winter warming

Global warming affects the Arctic much faster than the rest of the globe, with the highest amplitude of temperature rise occurring during the Polar Night. To determine the stress resistance of the ecosystem-engineering kelp Laminaria digitata against Arctic winter warming, non-meristematic discs of adult sporophytes from Porsangerfjorden (Finnmark, Norway) were kept in total darkness at 0°C and 5°C over a period of three months. The maximum photosynthetic quantum yield of photosystem II (Fv/Fm; Imaging-PAM, Walz GmbH Mess- und Regeltechnik, Effeltrich, Germany) was monitored once a week. For monitoring of potential growth, the size (area) of the algal discs was photographed every two weeks and analyzed with ImageJ (Version 1.52a). Every four weeks, subsamples for monitoring the dry weight and for the biochemical analyses were taken. Laminarin content was determined following via enzymatic digestion. C:N ratio was analyzed with an Elementar Analyzer. Mannitol concentration was analyzed using a HPLC. Absolut pigment concentrations were also analyzed using a HPLC and pool sizes, the de-epoxidation state of the xanthophyll cycle (DPS), and the ratios calculated afterwards.

BibTex:

@dataset{Trautmann_Moritz_and_Bartsch_Inka_and_Bligh_Margot_and_Buck-Wiese_Hagen_and_Hehemann_Jan-Hendrik_and_Niedzwiedz_Sarina_and_Plag_Niklas_and_Shan_Tifeng_and_Bischof_Kai_and_Diehl_Nora_2024,
    abstract = {Global warming affects the Arctic much faster than the rest of the globe, with the highest amplitude of temperature rise occurring during the Polar Night. To determine the stress resistance of the ecosystem-engineering kelp Laminaria digitata against Arctic winter warming, non-meristematic discs of adult sporophytes from Porsangerfjorden (Finnmark, Norway) were kept in total darkness at 0°C and 5°C over a period of three months. The maximum photosynthetic quantum yield of photosystem II (Fv/Fm; Imaging-PAM, Walz GmbH Mess- und Regeltechnik, Effeltrich, Germany) was monitored once a week. For monitoring of potential growth, the size (area) of the algal discs was photographed every two weeks and analyzed with ImageJ (Version 1.52a). Every four weeks, subsamples for monitoring the dry weight and for the biochemical analyses were taken. Laminarin content was determined following via enzymatic digestion. C:N ratio was analyzed with an Elementar Analyzer. Mannitol concentration was analyzed using a HPLC. Absolut pigment concentrations were also analyzed using a HPLC and pool sizes, the de-epoxidation state of the xanthophyll cycle (DPS), and the ratios calculated afterwards.},
    author = {Trautmann, Moritz and Bartsch, Inka and Bligh, Margot and Buck-Wiese, Hagen and Hehemann, Jan-Hendrik and Niedzwiedz, Sarina and Plag, Niklas and Shan, Tifeng and Bischof, Kai and Diehl, Nora},
    doi = {10.1594/PANGAEA.972789},
    institution = {PANGAEA (Ecology)},
    keyword = {'Arctic Amplification', 'CN', 'Fm', 'Fv', 'Mannitol', 'laminarin', 'pigments', 'polar night'},
    title = {Impact of climate change on the kelp Laminaria digitata: Biochemical and physiological raw data of a long-term experiment simulating Artic winter warming},
    url = {https://service.tib.eu/ldmservice/vdataset/png-doi-10-1594-pangaea-972789},
    year = {2024}
}