Changes
On August 4, 2023 at 8:52:49 AM UTC, admin:
-
No fields were updated. See the metadata diff for more details.
f | 1 | { | f | 1 | { |
2 | "author": "Schnabel, Arianne", | 2 | "author": "Schnabel, Arianne", | ||
3 | "author_email": "", | 3 | "author_email": "", | ||
4 | "creator_user_id": "17755db4-395a-4b3b-ac09-e8e3484ca700", | 4 | "creator_user_id": "17755db4-395a-4b3b-ac09-e8e3484ca700", | ||
5 | "doi": "10.22000/381", | 5 | "doi": "10.22000/381", | ||
6 | "doi_date_published": "2021", | 6 | "doi_date_published": "2021", | ||
7 | "doi_publisher": "", | 7 | "doi_publisher": "", | ||
8 | "doi_status": "True", | 8 | "doi_status": "True", | ||
9 | "extra_authors": [ | 9 | "extra_authors": [ | ||
10 | { | 10 | { | ||
11 | "extra_author": "Cotinguiba, Fernando", | 11 | "extra_author": "Cotinguiba, Fernando", | ||
12 | "orcid": "0000-0002-7006-0768" | 12 | "orcid": "0000-0002-7006-0768" | ||
13 | }, | 13 | }, | ||
14 | { | 14 | { | ||
15 | "extra_author": "Athmer, Benedikt", | 15 | "extra_author": "Athmer, Benedikt", | ||
16 | "orcid": "0000-0002-0234-4398" | 16 | "orcid": "0000-0002-0234-4398" | ||
17 | }, | 17 | }, | ||
18 | { | 18 | { | ||
19 | "extra_author": "Vogt, Thomas", | 19 | "extra_author": "Vogt, Thomas", | ||
20 | "orcid": "0000-0001-6155-3360" | 20 | "orcid": "0000-0001-6155-3360" | ||
21 | } | 21 | } | ||
22 | ], | 22 | ], | ||
23 | "groups": [], | 23 | "groups": [], | ||
24 | "id": "bae242ee-9081-4906-ad22-6b0a7cd073ab", | 24 | "id": "bae242ee-9081-4906-ad22-6b0a7cd073ab", | ||
25 | "isopen": false, | 25 | "isopen": false, | ||
26 | "license_id": "CC BY 4.0 Attribution", | 26 | "license_id": "CC BY 4.0 Attribution", | ||
27 | "license_title": "CC BY 4.0 Attribution", | 27 | "license_title": "CC BY 4.0 Attribution", | ||
28 | "metadata_created": "2023-01-12T13:30:30.983133", | 28 | "metadata_created": "2023-01-12T13:30:30.983133", | ||
t | 29 | "metadata_modified": "2023-08-04T08:51:21.454038", | t | 29 | "metadata_modified": "2023-08-04T08:52:49.460187", |
30 | "name": "rdr-doi-10-22000-381", | 30 | "name": "rdr-doi-10-22000-381", | ||
31 | "notes": "Abstract: Black pepper (Piper nigrum) is among the | 31 | "notes": "Abstract: Black pepper (Piper nigrum) is among the | ||
32 | world\u2019s most popular spices. Its pungent principle, piperine, has | 32 | world\u2019s most popular spices. Its pungent principle, piperine, has | ||
33 | already been identified 200 years ago, yet the biosynthesis of | 33 | already been identified 200 years ago, yet the biosynthesis of | ||
34 | piperine in black pepper remains largely enigmatic. In this report we | 34 | piperine in black pepper remains largely enigmatic. In this report we | ||
35 | analyzed the characteristic methylenedioxy bridge formation of the | 35 | analyzed the characteristic methylenedioxy bridge formation of the | ||
36 | aromatic part of piperine by a combination of RNA-sequencing, | 36 | aromatic part of piperine by a combination of RNA-sequencing, | ||
37 | functional expression in yeast, and LC-MS based analysis of substrate | 37 | functional expression in yeast, and LC-MS based analysis of substrate | ||
38 | and product profiles. We identified a single cytochrome P450 | 38 | and product profiles. We identified a single cytochrome P450 | ||
39 | transcript, specifically expressed in black pepper immature fruits. | 39 | transcript, specifically expressed in black pepper immature fruits. | ||
40 | The corresponding gene was functionally expressed in yeast | 40 | The corresponding gene was functionally expressed in yeast | ||
41 | (Saccharomyces cerevisiae) and characterized for substrate specificity | 41 | (Saccharomyces cerevisiae) and characterized for substrate specificity | ||
42 | with a series of putative aromatic precursors with an aromatic | 42 | with a series of putative aromatic precursors with an aromatic | ||
43 | vanilloid structure. Methylenedioxy bridge formation was only detected | 43 | vanilloid structure. Methylenedioxy bridge formation was only detected | ||
44 | when feruperic acid (5-(4-hydroxy-3-methoxyphenyl)-2,4-pentadienoic | 44 | when feruperic acid (5-(4-hydroxy-3-methoxyphenyl)-2,4-pentadienoic | ||
45 | acid) was used as a substrate, and the corresponding product was | 45 | acid) was used as a substrate, and the corresponding product was | ||
46 | identified as piperic acid. Two alternative precursors, ferulic acid | 46 | identified as piperic acid. Two alternative precursors, ferulic acid | ||
47 | and feruperine, were not accepted. Our data provide experimental | 47 | and feruperine, were not accepted. Our data provide experimental | ||
48 | evidence that formation of the piperine methylenedioxy bridge takes | 48 | evidence that formation of the piperine methylenedioxy bridge takes | ||
49 | place in young black pepper fruits after a currently hypothetical | 49 | place in young black pepper fruits after a currently hypothetical | ||
50 | chain elongation of ferulic acid and before the formation of the amide | 50 | chain elongation of ferulic acid and before the formation of the amide | ||
51 | bond. The partially characterized enzyme was classified as CYP719A37 | 51 | bond. The partially characterized enzyme was classified as CYP719A37 | ||
52 | and is discussed in terms of the phylogenetic origin of CYP719 | 52 | and is discussed in terms of the phylogenetic origin of CYP719 | ||
53 | catalysed reactions in magnoliids and eudicots.", | 53 | catalysed reactions in magnoliids and eudicots.", | ||
54 | "num_resources": 0, | 54 | "num_resources": 0, | ||
55 | "num_tags": 10, | 55 | "num_tags": 10, | ||
56 | "orcid": "0000-0002-2172-2905", | 56 | "orcid": "0000-0002-2172-2905", | ||
57 | "organization": { | 57 | "organization": { | ||
58 | "approval_status": "approved", | 58 | "approval_status": "approved", | ||
59 | "created": "2023-01-12T13:30:23.238233", | 59 | "created": "2023-01-12T13:30:23.238233", | ||
60 | "description": "RADAR (Research Data Repository) is a | 60 | "description": "RADAR (Research Data Repository) is a | ||
61 | cross-disciplinary repository for archiving and publishing research | 61 | cross-disciplinary repository for archiving and publishing research | ||
62 | data from completed scientific studies and projects. The focus is on | 62 | data from completed scientific studies and projects. The focus is on | ||
63 | research data from subjects that do not yet have their own | 63 | research data from subjects that do not yet have their own | ||
64 | discipline-specific infrastructures for research data management. ", | 64 | discipline-specific infrastructures for research data management. ", | ||
65 | "id": "013c89a9-383c-4200-8baa-0f78bf1d91f9", | 65 | "id": "013c89a9-383c-4200-8baa-0f78bf1d91f9", | ||
66 | "image_url": "radar-logo.svg", | 66 | "image_url": "radar-logo.svg", | ||
67 | "is_organization": true, | 67 | "is_organization": true, | ||
68 | "name": "radar", | 68 | "name": "radar", | ||
69 | "state": "active", | 69 | "state": "active", | ||
70 | "title": "RADAR", | 70 | "title": "RADAR", | ||
71 | "type": "organization" | 71 | "type": "organization" | ||
72 | }, | 72 | }, | ||
73 | "owner_org": "013c89a9-383c-4200-8baa-0f78bf1d91f9", | 73 | "owner_org": "013c89a9-383c-4200-8baa-0f78bf1d91f9", | ||
74 | "private": false, | 74 | "private": false, | ||
75 | "production_year": "2019-2020", | 75 | "production_year": "2019-2020", | ||
76 | "publication_year": "2021", | 76 | "publication_year": "2021", | ||
77 | "publishers": [ | 77 | "publishers": [ | ||
78 | { | 78 | { | ||
79 | "publisher": "Thomas Vogt" | 79 | "publisher": "Thomas Vogt" | ||
80 | } | 80 | } | ||
81 | ], | 81 | ], | ||
82 | "related_identifiers": [ | 82 | "related_identifiers": [ | ||
83 | { | 83 | { | ||
84 | "identifier": "10.3390/plants10010128", | 84 | "identifier": "10.3390/plants10010128", | ||
85 | "identifier_type": "DOI", | 85 | "identifier_type": "DOI", | ||
86 | "relation_type": "IsSupplementTo" | 86 | "relation_type": "IsSupplementTo" | ||
87 | } | 87 | } | ||
88 | ], | 88 | ], | ||
89 | "relationships_as_object": [], | 89 | "relationships_as_object": [], | ||
90 | "relationships_as_subject": [], | 90 | "relationships_as_subject": [], | ||
91 | "repository_name": "RADAR (Research Data Repository)", | 91 | "repository_name": "RADAR (Research Data Repository)", | ||
92 | "resource_type": "Dataset - chromatograms, raw data, excel files | 92 | "resource_type": "Dataset - chromatograms, raw data, excel files | ||
93 | (zipped)", | 93 | (zipped)", | ||
94 | "resources": [], | 94 | "resources": [], | ||
95 | "services_used_list": "", | 95 | "services_used_list": "", | ||
96 | "source_metadata_created": "2021", | 96 | "source_metadata_created": "2021", | ||
97 | "source_metadata_modified": "", | 97 | "source_metadata_modified": "", | ||
98 | "state": "active", | 98 | "state": "active", | ||
99 | "subject_areas": [ | 99 | "subject_areas": [ | ||
100 | { | 100 | { | ||
101 | "subject_area_additional": "", | 101 | "subject_area_additional": "", | ||
102 | "subject_area_name": "Biochemistry" | 102 | "subject_area_name": "Biochemistry" | ||
103 | } | 103 | } | ||
104 | ], | 104 | ], | ||
105 | "tags": [ | 105 | "tags": [ | ||
106 | { | 106 | { | ||
107 | "display_name": "Excel data", | 107 | "display_name": "Excel data", | ||
108 | "id": "76ca67b9-ee42-4f19-b9c6-74ff501cb1b0", | 108 | "id": "76ca67b9-ee42-4f19-b9c6-74ff501cb1b0", | ||
109 | "name": "Excel data", | 109 | "name": "Excel data", | ||
110 | "state": "active", | 110 | "state": "active", | ||
111 | "vocabulary_id": null | 111 | "vocabulary_id": null | ||
112 | }, | 112 | }, | ||
113 | { | 113 | { | ||
114 | "display_name": "Piper nigrum", | 114 | "display_name": "Piper nigrum", | ||
115 | "id": "c57dda35-7a22-4265-b5ba-0f8ef27d8aba", | 115 | "id": "c57dda35-7a22-4265-b5ba-0f8ef27d8aba", | ||
116 | "name": "Piper nigrum", | 116 | "name": "Piper nigrum", | ||
117 | "state": "active", | 117 | "state": "active", | ||
118 | "vocabulary_id": null | 118 | "vocabulary_id": null | ||
119 | }, | 119 | }, | ||
120 | { | 120 | { | ||
121 | "display_name": "black pepper", | 121 | "display_name": "black pepper", | ||
122 | "id": "4c53c72a-d8ee-4b29-bcbf-efda1405b903", | 122 | "id": "4c53c72a-d8ee-4b29-bcbf-efda1405b903", | ||
123 | "name": "black pepper", | 123 | "name": "black pepper", | ||
124 | "state": "active", | 124 | "state": "active", | ||
125 | "vocabulary_id": null | 125 | "vocabulary_id": null | ||
126 | }, | 126 | }, | ||
127 | { | 127 | { | ||
128 | "display_name": "cytochrome P450", | 128 | "display_name": "cytochrome P450", | ||
129 | "id": "c5b3075c-798d-4a45-bae1-961c9ab5f73d", | 129 | "id": "c5b3075c-798d-4a45-bae1-961c9ab5f73d", | ||
130 | "name": "cytochrome P450", | 130 | "name": "cytochrome P450", | ||
131 | "state": "active", | 131 | "state": "active", | ||
132 | "vocabulary_id": null | 132 | "vocabulary_id": null | ||
133 | }, | 133 | }, | ||
134 | { | 134 | { | ||
135 | "display_name": "enzyme activity", | 135 | "display_name": "enzyme activity", | ||
136 | "id": "5444a8c0-f0d5-4bc0-8e1b-e482795b60bd", | 136 | "id": "5444a8c0-f0d5-4bc0-8e1b-e482795b60bd", | ||
137 | "name": "enzyme activity", | 137 | "name": "enzyme activity", | ||
138 | "state": "active", | 138 | "state": "active", | ||
139 | "vocabulary_id": null | 139 | "vocabulary_id": null | ||
140 | }, | 140 | }, | ||
141 | { | 141 | { | ||
142 | "display_name": "methylenedioxy bridge", | 142 | "display_name": "methylenedioxy bridge", | ||
143 | "id": "a3ed4bc0-5bb6-4d64-b575-685462bc7337", | 143 | "id": "a3ed4bc0-5bb6-4d64-b575-685462bc7337", | ||
144 | "name": "methylenedioxy bridge", | 144 | "name": "methylenedioxy bridge", | ||
145 | "state": "active", | 145 | "state": "active", | ||
146 | "vocabulary_id": null | 146 | "vocabulary_id": null | ||
147 | }, | 147 | }, | ||
148 | { | 148 | { | ||
149 | "display_name": "piperine", | 149 | "display_name": "piperine", | ||
150 | "id": "7ba8940f-0497-4d2c-b20f-8bd101e2564a", | 150 | "id": "7ba8940f-0497-4d2c-b20f-8bd101e2564a", | ||
151 | "name": "piperine", | 151 | "name": "piperine", | ||
152 | "state": "active", | 152 | "state": "active", | ||
153 | "vocabulary_id": null | 153 | "vocabulary_id": null | ||
154 | }, | 154 | }, | ||
155 | { | 155 | { | ||
156 | "display_name": "raw data chromatograms", | 156 | "display_name": "raw data chromatograms", | ||
157 | "id": "7e5dff6b-d2c1-46f9-a52b-db4f4194e7ed", | 157 | "id": "7e5dff6b-d2c1-46f9-a52b-db4f4194e7ed", | ||
158 | "name": "raw data chromatograms", | 158 | "name": "raw data chromatograms", | ||
159 | "state": "active", | 159 | "state": "active", | ||
160 | "vocabulary_id": null | 160 | "vocabulary_id": null | ||
161 | }, | 161 | }, | ||
162 | { | 162 | { | ||
163 | "display_name": "sequence data", | 163 | "display_name": "sequence data", | ||
164 | "id": "4b8e45da-25a4-465e-9771-ebfa71fe5c8c", | 164 | "id": "4b8e45da-25a4-465e-9771-ebfa71fe5c8c", | ||
165 | "name": "sequence data", | 165 | "name": "sequence data", | ||
166 | "state": "active", | 166 | "state": "active", | ||
167 | "vocabulary_id": null | 167 | "vocabulary_id": null | ||
168 | }, | 168 | }, | ||
169 | { | 169 | { | ||
170 | "display_name": "yeast expression", | 170 | "display_name": "yeast expression", | ||
171 | "id": "c80638bb-ca9c-4213-8713-d144d92ca35e", | 171 | "id": "c80638bb-ca9c-4213-8713-d144d92ca35e", | ||
172 | "name": "yeast expression", | 172 | "name": "yeast expression", | ||
173 | "state": "active", | 173 | "state": "active", | ||
174 | "vocabulary_id": null | 174 | "vocabulary_id": null | ||
175 | } | 175 | } | ||
176 | ], | 176 | ], | ||
177 | "title": "Plants cyp719 radar", | 177 | "title": "Plants cyp719 radar", | ||
178 | "type": "vdataset", | 178 | "type": "vdataset", | ||
179 | "url": "https://doi.org/10.22000/381" | 179 | "url": "https://doi.org/10.22000/381" | ||
180 | } | 180 | } |