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{
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{
            
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  "author": "Milde, Raika",
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  "author": "Milde, Raika",
            
3
  "author_email": "",
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  "author_email": "",
            
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  "creator_user_id": "17755db4-395a-4b3b-ac09-e8e3484ca700",
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  "creator_user_id": "17755db4-395a-4b3b-ac09-e8e3484ca700",
            
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  "doi": "10.22000/799",
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  "doi": "10.22000/799",
            
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  "doi_date_published": "2022",
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  "doi_date_published": "2022",
            
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  "doi_publisher": "",
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  "doi_publisher": "",
            
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  "doi_status": "True",
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  "doi_status": "True",
            
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  "extra_authors": [
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  "extra_authors": [
            
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    {
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    {
            
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      "extra_author": "Schnabel, Arianne",
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      "extra_author": "Schnabel, Arianne",
            
12
      "orcid": "0000-0002-2172-2905"
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      "orcid": "0000-0002-2172-2905"
            
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    },
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    },
            
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    {
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    {
            
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      "extra_author": "Ditfe, Toni",
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      "extra_author": "Ditfe, Toni",
            
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      "orcid": "0000-0001-6360-6673"
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      "orcid": "0000-0001-6360-6673"
            
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    },
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    },
            
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    {
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    {
            
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      "extra_author": "Hoehenwarter, Wolfgang",
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      "extra_author": "Hoehenwarter, Wolfgang",
            
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      "orcid": "0000-0002-7669-7524"
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      "orcid": "0000-0002-7669-7524"
            
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    },
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    },
            
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    {
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    {
            
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      "extra_author": "Proksch, Carsten",
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      "extra_author": "Proksch, Carsten",
            
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      "orcid": "0000-0003-4948-8281"
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      "orcid": "0000-0003-4948-8281"
            
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    },
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    },
            
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    {
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    {
            
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      "extra_author": "Westermann, Bernhard",
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      "extra_author": "Westermann, Bernhard",
            
28
      "orcid": "0000-0002-6228-5991"
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      "orcid": "0000-0002-6228-5991"
            
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    },
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    },
            
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    {
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    {
            
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      "extra_author": "Vogt, Thomas",
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      "extra_author": "Vogt, Thomas",
            
32
      "orcid": "0000-0001-6155-3360"
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      "orcid": "0000-0001-6155-3360"
            
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    }
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    }
            
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  ],
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  ],
            
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  "groups": [],
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  "groups": [],
            
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  "id": "16693d9f-07e1-4ff1-9ab6-87ca53305e1a",
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  "id": "16693d9f-07e1-4ff1-9ab6-87ca53305e1a",
            
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  "isopen": false,
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  "isopen": false,
            
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  "license_id": "CC BY 4.0 Attribution",
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  "license_id": "CC BY 4.0 Attribution",
            
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  "license_title": "CC BY 4.0 Attribution",
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  "license_title": "CC BY 4.0 Attribution",
            
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  "metadata_created": "2023-01-12T13:30:54.420773",
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  "metadata_created": "2023-01-12T13:30:54.420773",
            
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  "metadata_modified": "2023-08-04T08:51:32.610078",
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  "metadata_modified": "2023-08-04T08:53:01.536555",
            
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  "name": "rdr-doi-10-22000-799",
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  "name": "rdr-doi-10-22000-799",
            
43
  "notes": "Abstract: Capsaicin, produced by diverse Capsicum species 
43
  "notes": "Abstract: Capsaicin, produced by diverse Capsicum species 
            
44
is among the world\u2019s most popular spices and of considerable 
44
is among the world\u2019s most popular spices and of considerable 
            
45
pharmaceutical relevance. Although the capsaicinoid biosynthetic 
45
pharmaceutical relevance. Although the capsaicinoid biosynthetic 
            
46
pathway has been investigated for decades, several biosynthetic steps 
46
pathway has been investigated for decades, several biosynthetic steps 
            
47
have remained partly hypothetical. Genetic evidence suggested that the 
47
have remained partly hypothetical. Genetic evidence suggested that the 
            
48
decisive capsaicin synthase is encoded by the Pun1 locus. Yet, genetic 
48
decisive capsaicin synthase is encoded by the Pun1 locus. Yet, genetic 
            
49
evidence of the Pun1 locus was never corroborated by functionally 
49
evidence of the Pun1 locus was never corroborated by functionally 
            
50
active capsaicin synthase that presumably catalyzes amide bond 
50
active capsaicin synthase that presumably catalyzes amide bond 
            
51
formation between trans 8-methyl-6-nonenoyl-CoA derived from branched 
51
formation between trans 8-methyl-6-nonenoyl-CoA derived from branched 
            
52
chain amino acid biosynthesis and vanilloylamine derived from the 
52
chain amino acid biosynthesis and vanilloylamine derived from the 
            
53
phenylpropanoid pathway. In this report, we demonstrate enzymatic 
53
phenylpropanoid pathway. In this report, we demonstrate enzymatic 
            
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activity of a recombinant capsaicin synthase encoded by Pun1, 
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activity of a recombinant capsaicin synthase encoded by Pun1, 
            
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functionally expressed in E. coli, and provide information on 
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functionally expressed in E. coli, and provide information on 
            
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substrate specificity and catalytic properties. Recombinant capsaicin 
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substrate specificity and catalytic properties. Recombinant capsaicin 
            
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synthase is specific for selected aliphatic CoA-esters and highly 
57
synthase is specific for selected aliphatic CoA-esters and highly 
            
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specific for vanilloylamine. Partly purified from E. coli, the 
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specific for vanilloylamine. Partly purified from E. coli, the 
            
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recombinant active enzyme is a monomeric protein of 51 kDa that is 
59
recombinant active enzyme is a monomeric protein of 51 kDa that is 
            
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independent of additional co-factors or associated proteins, as 
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independent of additional co-factors or associated proteins, as 
            
61
previously proposed. These data can now be used to design capsaicin 
61
previously proposed. These data can now be used to design capsaicin 
            
62
synthase variants with different properties and alternative substrate 
62
synthase variants with different properties and alternative substrate 
            
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preferences.",
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preferences.",
            
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  "num_resources": 0,
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  "num_resources": 0,
            
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  "num_tags": 8,
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  "num_tags": 8,
            
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  "orcid": "0000-0003-0630-8105",
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  "orcid": "0000-0003-0630-8105",
            
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  "organization": {
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  "organization": {
            
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    "approval_status": "approved",
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    "approval_status": "approved",
            
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    "created": "2023-01-12T13:30:23.238233",
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    "created": "2023-01-12T13:30:23.238233",
            
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    "description": "RADAR (Research Data Repository) is a 
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    "description": "RADAR (Research Data Repository) is a 
            
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cross-disciplinary repository for archiving and publishing research 
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cross-disciplinary repository for archiving and publishing research 
            
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data from completed scientific studies and projects. The focus is on 
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data from completed scientific studies and projects. The focus is on 
            
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research data from subjects that do not yet have their own 
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research data from subjects that do not yet have their own 
            
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discipline-specific infrastructures for research data management. ",
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discipline-specific infrastructures for research data management. ",
            
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    "id": "013c89a9-383c-4200-8baa-0f78bf1d91f9",
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    "id": "013c89a9-383c-4200-8baa-0f78bf1d91f9",
            
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    "image_url": "radar-logo.svg",
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    "image_url": "radar-logo.svg",
            
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    "is_organization": true,
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    "is_organization": true,
            
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    "name": "radar",
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    "name": "radar",
            
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    "state": "active",
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    "state": "active",
            
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    "title": "RADAR",
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    "title": "RADAR",
            
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    "type": "organization"
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    "type": "organization"
            
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  },
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  },
            
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  "owner_org": "013c89a9-383c-4200-8baa-0f78bf1d91f9",
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  "owner_org": "013c89a9-383c-4200-8baa-0f78bf1d91f9",
            
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  "private": false,
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  "private": false,
            
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  "production_year": "2022",
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  "production_year": "2022",
            
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  "publication_year": "2022",
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  "publication_year": "2022",
            
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  "publishers": [
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  "publishers": [
            
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    {
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    {
            
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      "publisher": "Vogt, Thomas"
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      "publisher": "Vogt, Thomas"
            
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    }
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    }
            
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  ],
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  ],
            
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  "related_identifiers": [
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  "related_identifiers": [
            
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    {
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    {
            
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      "identifier": "10.3390/molecules27206878",
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      "identifier": "10.3390/molecules27206878",
            
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      "identifier_type": "DOI",
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      "identifier_type": "DOI",
            
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      "relation_type": "IsSupplementTo"
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      "relation_type": "IsSupplementTo"
            
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    {
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    {
            
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      "identifier": 
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      "identifier": 
            
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"https://identifiers.org/pubchem.compound:1548943",
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"https://identifiers.org/pubchem.compound:1548943",
            
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      "identifier_type": "URL",
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      "identifier_type": "URL",
            
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      "relation_type": "Documents"
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      "relation_type": "Documents"
            
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      "identifier": "https://identifiers.org/pubchem.compound:165576",
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      "identifier": "https://identifiers.org/pubchem.compound:165576",
            
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      "identifier_type": "URL",
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      "identifier_type": "URL",
            
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      "relation_type": "Documents"
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      "relation_type": "Documents"
            
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    }
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  ],
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  "relationships_as_object": [],
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  "relationships_as_object": [],
            
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  "relationships_as_subject": [],
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  "relationships_as_subject": [],
            
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  "repository_name": "RADAR (Research Data Repository)",
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  "repository_name": "RADAR (Research Data Repository)",
            
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  "resource_type": "Dataset - chromatogram data (arw files), protein 
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  "resource_type": "Dataset - chromatogram data (arw files), protein 
            
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LC-MS/MS, images (tiff, jpeg), tabular data (csv, xlsx)",
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LC-MS/MS, images (tiff, jpeg), tabular data (csv, xlsx)",
            
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  "resources": [],
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  "resources": [],
            
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  "services_used_list": "",
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  "services_used_list": "",
            
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  "source_metadata_created": "2022",
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  "source_metadata_created": "2022",
            
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  "source_metadata_modified": "",
            
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  "state": "active",
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  "state": "active",
            
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  "subject_areas": [
            
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    {
            
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      "subject_area_additional": "",
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      "subject_area_additional": "",
            
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      "subject_area_name": "Biochemistry"
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      "subject_area_name": "Biochemistry"
            
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    }
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    }
            
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  ],
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  ],
            
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  "tags": [
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  "tags": [
            
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    {
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    {
            
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      "display_name": "BAHD",
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      "display_name": "BAHD",
            
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      "id": "8a5f3d7c-afd7-44ed-9792-a3ae0a885b35",
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      "id": "8a5f3d7c-afd7-44ed-9792-a3ae0a885b35",
            
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      "name": "BAHD",
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      "name": "BAHD",
            
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      "state": "active",
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      "state": "active",
            
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      "vocabulary_id": null
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      "vocabulary_id": null
            
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    },
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    },
            
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    {
134
    {
            
135
      "display_name": "Capsaicin",
135
      "display_name": "Capsaicin",
            
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      "id": "bbd5ccfb-a796-4f5f-bc95-6bc2f1fa65c9",
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      "id": "bbd5ccfb-a796-4f5f-bc95-6bc2f1fa65c9",
            
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      "name": "Capsaicin",
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      "name": "Capsaicin",
            
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      "state": "active",
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      "state": "active",
            
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      "vocabulary_id": null
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      "vocabulary_id": null
            
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    },
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    },
            
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    {
141
    {
            
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      "display_name": "Capsicum",
142
      "display_name": "Capsicum",
            
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      "id": "6d9c4e69-e04b-4638-961b-f60439d47b71",
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      "id": "6d9c4e69-e04b-4638-961b-f60439d47b71",
            
144
      "name": "Capsicum",
144
      "name": "Capsicum",
            
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      "state": "active",
145
      "state": "active",
            
146
      "vocabulary_id": null
146
      "vocabulary_id": null
            
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    },
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    },
            
148
    {
148
    {
            
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      "display_name": "CoA-ester",
149
      "display_name": "CoA-ester",
            
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      "id": "9a4b1c85-6dff-4e36-9965-0bea1040d0af",
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      "id": "9a4b1c85-6dff-4e36-9965-0bea1040d0af",
            
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      "name": "CoA-ester",
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      "name": "CoA-ester",
            
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      "state": "active",
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      "state": "active",
            
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      "vocabulary_id": null
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      "vocabulary_id": null
            
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    },
154
    },
            
155
    {
155
    {
            
156
      "display_name": "Pun1",
156
      "display_name": "Pun1",
            
157
      "id": "0c0118f4-3109-4e15-8d13-6e7271a87ffb",
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      "id": "0c0118f4-3109-4e15-8d13-6e7271a87ffb",
            
158
      "name": "Pun1",
158
      "name": "Pun1",
            
159
      "state": "active",
159
      "state": "active",
            
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      "vocabulary_id": null
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      "vocabulary_id": null
            
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    },
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    },
            
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    {
162
    {
            
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      "display_name": "acyltransferase",
163
      "display_name": "acyltransferase",
            
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      "id": "228fc1dc-1a69-4d85-aac6-4724f2a4110d",
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      "id": "228fc1dc-1a69-4d85-aac6-4724f2a4110d",
            
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      "name": "acyltransferase",
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      "name": "acyltransferase",
            
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      "state": "active",
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      "state": "active",
            
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      "vocabulary_id": null
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      "vocabulary_id": null
            
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    },
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    },
            
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    {
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    {
            
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      "display_name": "enzyme activity",
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      "display_name": "enzyme activity",
            
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      "id": "5444a8c0-f0d5-4bc0-8e1b-e482795b60bd",
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      "id": "5444a8c0-f0d5-4bc0-8e1b-e482795b60bd",
            
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      "name": "enzyme activity",
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      "name": "enzyme activity",
            
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      "state": "active",
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      "state": "active",
            
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      "vocabulary_id": null
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      "vocabulary_id": null
            
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    },
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    },
            
176
    {
176
    {
            
177
      "display_name": "substrate specificity",
177
      "display_name": "substrate specificity",
            
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      "id": "195b59a9-46ce-4489-83a8-aeb4b1680fed",
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      "id": "195b59a9-46ce-4489-83a8-aeb4b1680fed",
            
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      "name": "substrate specificity",
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      "name": "substrate specificity",
            
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      "state": "active",
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      "state": "active",
            
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      "vocabulary_id": null
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      "vocabulary_id": null
            
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    }
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    }
            
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  ],
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  ],
            
184
  "title": "supporting information: chemical synthesis of trans 
184
  "title": "supporting information: chemical synthesis of trans 
            
185
8-methyl-6-nonenoyl-coa and functional expression unravel capsaicin 
185
8-methyl-6-nonenoyl-coa and functional expression unravel capsaicin 
            
186
synthase activity encoded by the pun1 locus",
186
synthase activity encoded by the pun1 locus",
            
187
  "type": "vdataset",
187
  "type": "vdataset",
            
188
  "url": "https://doi.org/10.22000/799"
188
  "url": "https://doi.org/10.22000/799"
            
189
}
189
}