Colimitation of the unicellular photosynthetic diazotroph Crocosphaera watsonii by phosphorus, light, and carbon dioxide

We describe interactive effects of total phosphorus (total P = 0.1-4.0 µmol/L; added as H2NaPO4), irradiance (40 and 150 µmol quanta/m**2/s), and the partial pressure of carbon dioxide (P-CO2; 19 and 81 Pa, i.e., 190 and 800 ppm) on growth and CO2- and dinitrogen (N-2)-fixation rates of the unicellular N-2-fixing cyanobacterium Crocosphaera watsonii (WH0003) isolated from the Pacific Ocean near Hawaii. In semicontinuous cultures of C. watsonii, elevated P-CO2 positively affected growth and CO2- and N-2-fixation rates under high light. Under low light, elevated P-CO2 positively affected growth rates at all concentrations of P, but CO2- and N-2-fixation rates were affected by elevated P-CO2 only when P was low. In both high-light and low-light cultures, the total P requirements for growth and CO2- and N-2-fixation declined as P-CO2 increased. The minimum concentration (C-min) of total P and half-saturation constant (K-1/2) for growth and CO2- and N-2-fixation rates with respect to total P were reduced by 0.05 µmol/L as a function of elevated P-CO2. We speculate that low P requirements under high P-CO2 resulted from a lower energy demand associated with carbon-concentrating mechanisms in comparison with low-P-CO2 cultures. There was also a 0.10 µmol/L increase in C-min and K-1/2 for growth and N-2 fixation with respect to total P as a function of increasing light regardless of P-CO2 concentration. We speculate that cellular P concentrations are responsible for this shift through biodilution of cellular P and possibly cellular P uptake systems as a function of increasing light. Changing concentrations of P, CO2, and light have both positive and negative interactive effects on growth and CO2-, and N-2-fixation rates of unicellular oxygenic diazotrophs like C. watsonii.

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Garcia, Nathan S, Fu, Feixue, Hutchins, David A (2013). Dataset: Colimitation of the unicellular photosynthetic diazotroph Crocosphaera watsonii by phosphorus, light, and carbon dioxide. https://doi.org/10.1594/PANGAEA.835394

DOI retrieved: 2013

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Field Value
Imported on November 30, 2024
Last update November 30, 2024
License CC-BY-3.0
Source https://doi.org/10.1594/PANGAEA.835394
Author Garcia, Nathan S
Given Name Nathan S
Family Name Garcia
More Authors
Fu, Feixue
Hutchins, David A
Source Creation 2013
Publication Year 2013
Resource Type text/tab-separated-values - filename: Garcia_2013
Subject Areas
Name: BiologicalClassification

Name: Chemistry

Name: Oceans

Related Identifiers
Title: Colimitation of the unicellular photosynthetic diazotroph Crocosphaera watsonii by phosphorus, light, and carbon dioxide
Identifier: https://doi.org/10.4319/lo.2013.58.4.1501
Type: DOI
Relation: References
Year: 2013
Source: Limnology and Oceanography
Authors: Garcia Nathan S , Fu Feixue , Hutchins David A , Hutchins David A , Fu Feixue , Lavigne Héloïse , Epitalon Jean-Marie , Gattuso Jean-Pierre .

Title: Deployment: lab Hutchins 07-12 diazotrophs
Identifier: https://www.bco-dmo.org/deployment/59043
Type: DOI
Relation: References
Year: 2013
Source: Biological and Chemical Oceanography Data Management Office
Authors: Garcia Nathan S , Fu Feixue , Hutchins David A , Hutchins David A , Fu Feixue , Lavigne Héloïse , Epitalon Jean-Marie , Gattuso Jean-Pierre .

Title: seacarb: seawater carbonate chemistry with R. R package version 3.0
Identifier: https://cran.r-project.org/package=seacarb
Type: DOI
Relation: References
Year: 2014
Authors: Garcia Nathan S , Fu Feixue , Hutchins David A , Hutchins David A , Fu Feixue , Lavigne Héloïse , Epitalon Jean-Marie , Gattuso Jean-Pierre .