Sialidase assay and analysis of free sialic acid in tsetse (Glossina sp.) collected in protected and tsetse invested areas in Nigeria

doi:doi:10.1594/PANGAEA.913420

Sialidase assay and analysis of free sialic acid in tsetse (Glossina sp.) collected in protected and tsetse invested areas in Nigeria

This data set includes results of a sialidase assay applied in tsetse gut tissue from a tsetse (Glossina sp.) collection carried out in Nigeria between 2014 and 2016. Traps were employed for one to three days in four different National Parks, one Game Reserve and two tsetse invested areas. Each tsetse was identified morphologically and dissected in the field, all tissues preserved and used for further analysis. The whole gut of each tsetse was homogenized in the field and partially preserved for DNA analysis and used to investigate for sialidase activity with a fluorescent substrate and a natural substrate. Analysis for sialidase activity was done by fluorescent measurement and reverse phase HPLC of processed gut samples.

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Cite this as

Weber, Judith Sophie, Shaida, Stephen Saikiu, Ngomtcho, Sen Claudine Henriette, Musa, Usman Baba, Adamu, Ahmadu, Gbem, Thaddeus Terlumun, Berger, Petra, Mamman, Mohammed, Nok, Jonathan Andrew, Kelm, Sørge (2020). Dataset: Sialidase assay and analysis of free sialic acid in tsetse (Glossina sp.) collected in protected and tsetse invested areas in Nigeria. https://doi.org/10.1594/PANGAEA.913420

DOI retrieved: 2020

Additional Info

Field	Value
Imported on	November 29, 2024
Last update	November 29, 2024
License	CC-BY-4.0
Source	https://doi.org/10.1594/PANGAEA.913420
Author	Weber, Judith Sophie
Given Name	Judith Sophie
Family Name	Weber
More Authors	Shaida, Stephen Saikiu Ngomtcho, Sen Claudine Henriette Musa, Usman Baba Adamu, Ahmadu Gbem, Thaddeus Terlumun Berger, Petra Mamman, Mohammed Nok, Jonathan Andrew Kelm, Sørge
Source Creation	2020
Publication Year	2020
Resource Type	text/tab-separated-values - filename: Tsetse_free_sia_Nigeria
Subject Areas	Name: Biosphere
Related Identifiers	Title: Diversity and phylogenetic relationships of Glossina populations in Nigeria and the Cameroonian border region Identifier: https://doi.org/10.1186/s12866-018-1293-6 Type: DOI Relation: References Year: 2018 Source: BMC Microbiology Authors: Shaida Stephen Saikiu , Weber Judith Sophie , Gbem Thaddeus Terlumun , Ngomtcho Sen Claudine Henriette , Musa Usman Baba , Achukwi Mbunkah Daniel , Mamman Mohammed , Ndams Iliya Shehu , Nok Jonathan Andrew , Kelm Sørge , Hara Shuuji , Yamaguchi Masatoshi , Takemori Yasuyo , Yoshitake Takashi , Nakamura Masaru , Wang Libo , Wang John D , Zhou Xiang , Wu Lijun , Sun Xue-Long . Title: Tsetse fly sampling, tissue and analysis codes used in Weber et al., 2020 Identifier: https://store.pangaea.de/Publications/Weber-etal_2020/Fly_sample_overview_codes.pdf Type: DOI Relation: References Authors: Shaida Stephen Saikiu , Weber Judith Sophie , Gbem Thaddeus Terlumun , Ngomtcho Sen Claudine Henriette , Musa Usman Baba , Achukwi Mbunkah Daniel , Mamman Mohammed , Ndams Iliya Shehu , Nok Jonathan Andrew , Kelm Sørge , Hara Shuuji , Yamaguchi Masatoshi , Takemori Yasuyo , Yoshitake Takashi , Nakamura Masaru , Wang Libo , Wang John D , Zhou Xiang , Wu Lijun , Sun Xue-Long . Title: 1,2-diamino-4,5-methylenedioxybenzene as a highly sensitive fluorogenic reagent for α-dicarbonyl compounds Identifier: https://doi.org/10.1016/S0003-2670(00)85285-X Type: DOI Relation: References Year: 1988 Source: Analytica Chimica Acta Authors: Shaida Stephen Saikiu , Weber Judith Sophie , Gbem Thaddeus Terlumun , Ngomtcho Sen Claudine Henriette , Musa Usman Baba , Achukwi Mbunkah Daniel , Mamman Mohammed , Ndams Iliya Shehu , Nok Jonathan Andrew , Kelm Sørge , Hara Shuuji , Yamaguchi Masatoshi , Takemori Yasuyo , Yoshitake Takashi , Nakamura Masaru , Wang Libo , Wang John D , Zhou Xiang , Wu Lijun , Sun Xue-Long . Title: Systematic investigation of quinoxaline derivatization of sialic acids and their quantitation applicability using high performance liquid chromatography Identifier: https://doi.org/10.1039/c4ra08930h Type: DOI Relation: References Year: 2014 Source: RSC Advances Authors: Shaida Stephen Saikiu , Weber Judith Sophie , Gbem Thaddeus Terlumun , Ngomtcho Sen Claudine Henriette , Musa Usman Baba , Achukwi Mbunkah Daniel , Mamman Mohammed , Ndams Iliya Shehu , Nok Jonathan Andrew , Kelm Sørge , Hara Shuuji , Yamaguchi Masatoshi , Takemori Yasuyo , Yoshitake Takashi , Nakamura Masaru , Wang Libo , Wang John D , Zhou Xiang , Wu Lijun , Sun Xue-Long .