Tryapanosoma prevalence in tsetse proboscis (Glossina sp.) collected in protected and tsetse invested areas in Nigeria

doi:doi:10.1594/PANGAEA.913422

Tryapanosoma prevalence in tsetse proboscis (Glossina sp.) collected in protected and tsetse invested areas in Nigeria

This data set includes the results of nested PCR-analysis of tsetse (Glossina sp.) proboscis samples with primers described by Adams et.al. (2006) and Hamilton et al (2004) from a tsetse (Glossina sp.) collection carried out in Nigeria between 2014 and 2016. Traps were employed for one to three days in four different National Parks, one Game Reserve and two tsetse invested areas. Each tsetse was identified morphologically and dissected in the field. DNA was crudely extracted from the proboscis by Proteinase K digest. Presence of trypanosomes was investigated by amplification of Internal Transcribed Spacer-1 (ITS1). Identification of the respective trypanosome species was done based on lengths polymorphism of the ITS1 region by estimating the approximate size of the DNA amplicon based on electrophoretic separation in agarose gels, as well as sequencing of selected ITS1 amplicons and additional glycosomal Glyceraldehyde-3-phosohate dehydrogenase.

Data and Resources

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Cite this as

Weber, Judith Sophie, Shaida, Stephen Saikiu, Ngomtcho, Sen Claudine Henriette, Musa, Usman Baba, Adamu, Ahmadu, Gbem, Thaddeus Terlumun, Berger, Petra, Mamman, Mohammed, Nok, Jonathan Andrew, Kelm, Sørge (2020). Dataset: Tryapanosoma prevalence in tsetse proboscis (Glossina sp.) collected in protected and tsetse invested areas in Nigeria. https://doi.org/10.1594/PANGAEA.913422

DOI retrieved: 2020

Additional Info

Field	Value
Imported on	November 29, 2024
Last update	November 29, 2024
License	CC-BY-4.0
Source	https://doi.org/10.1594/PANGAEA.913422
Author	Weber, Judith Sophie
Given Name	Judith Sophie
Family Name	Weber
More Authors	Shaida, Stephen Saikiu Ngomtcho, Sen Claudine Henriette Musa, Usman Baba Adamu, Ahmadu Gbem, Thaddeus Terlumun Berger, Petra Mamman, Mohammed Nok, Jonathan Andrew Kelm, Sørge
Source Creation	2020
Publication Year	2020
Resource Type	application/zip - filename: Tsetse_Nigeria_biosample_analyse_proboscis
Subject Areas	Name: Biosphere
Related Identifiers	Title: Diversity and phylogenetic relationships of Glossina populations in Nigeria and the Cameroonian border region Identifier: https://doi.org/10.1186/s12866-018-1293-6 Type: DOI Relation: References Year: 2018 Source: BMC Microbiology Authors: Shaida Stephen Saikiu , Weber Judith Sophie , Gbem Thaddeus Terlumun , Ngomtcho Sen Claudine Henriette , Musa Usman Baba , Achukwi Mbunkah Daniel , Mamman Mohammed , Ndams Iliya Shehu , Nok Jonathan Andrew , Kelm Sørge , Weber Judith Sophie , Ngomtcho Sen Claudine Henriette , Shaida Stephen Saikiu , Chechet Gloria Dada , Gbem Thaddeus Terlumun , Nok Jonathan Andrew , Mamman Mohammed , Achukwi Mbunkah Daniel , Kelm Sørge , Adams Emily R , Malele Imna I , Msangi Atway R , Gibson Wendy C , Hamilton Patrick B , Stevens Jamie R , Gaunt Michael W , Gidley Jennifer , Gibson Wendy C , Ngomtcho Sen Claudine Henriette , Weber Judith Sophie , Ngo Bum Elisabeth , Gbem Thaddeus Terlumun , Kelm Sørge , Achukwi Mbunkah Daniel . Title: Genetic diversity of trypanosome species in tsetse flies (Glossina spp.) in Nigeria Identifier: https://doi.org/10.1186/s13071-019-3718-y Type: DOI Relation: References Year: 2019 Source: Parasites & Vectors Authors: Shaida Stephen Saikiu , Weber Judith Sophie , Gbem Thaddeus Terlumun , Ngomtcho Sen Claudine Henriette , Musa Usman Baba , Achukwi Mbunkah Daniel , Mamman Mohammed , Ndams Iliya Shehu , Nok Jonathan Andrew , Kelm Sørge , Weber Judith Sophie , Ngomtcho Sen Claudine Henriette , Shaida Stephen Saikiu , Chechet Gloria Dada , Gbem Thaddeus Terlumun , Nok Jonathan Andrew , Mamman Mohammed , Achukwi Mbunkah Daniel , Kelm Sørge , Adams Emily R , Malele Imna I , Msangi Atway R , Gibson Wendy C , Hamilton Patrick B , Stevens Jamie R , Gaunt Michael W , Gidley Jennifer , Gibson Wendy C , Ngomtcho Sen Claudine Henriette , Weber Judith Sophie , Ngo Bum Elisabeth , Gbem Thaddeus Terlumun , Kelm Sørge , Achukwi Mbunkah Daniel . Title: Tsetse fly sampling, tissue and analysis codes used in Weber et al., 2020 Identifier: https://store.pangaea.de/Publications/Weber-etal_2020/Fly_sample_overview_codes.pdf Type: DOI Relation: References Authors: Shaida Stephen Saikiu , Weber Judith Sophie , Gbem Thaddeus Terlumun , Ngomtcho Sen Claudine Henriette , Musa Usman Baba , Achukwi Mbunkah Daniel , Mamman Mohammed , Ndams Iliya Shehu , Nok Jonathan Andrew , Kelm Sørge , Weber Judith Sophie , Ngomtcho Sen Claudine Henriette , Shaida Stephen Saikiu , Chechet Gloria Dada , Gbem Thaddeus Terlumun , Nok Jonathan Andrew , Mamman Mohammed , Achukwi Mbunkah Daniel , Kelm Sørge , Adams Emily R , Malele Imna I , Msangi Atway R , Gibson Wendy C , Hamilton Patrick B , Stevens Jamie R , Gaunt Michael W , Gidley Jennifer , Gibson Wendy C , Ngomtcho Sen Claudine Henriette , Weber Judith Sophie , Ngo Bum Elisabeth , Gbem Thaddeus Terlumun , Kelm Sørge , Achukwi Mbunkah Daniel . Title: Trypanosome identification in wild tsetse populations in Tanzania using generic primers to amplify the ribosomal RNA ITS-1 region Identifier: https://doi.org/10.1016/j.actatropica.2006.10.002 Type: DOI Relation: References Year: 2006 Source: Acta Tropica Authors: Shaida Stephen Saikiu , Weber Judith Sophie , Gbem Thaddeus Terlumun , Ngomtcho Sen Claudine Henriette , Musa Usman Baba , Achukwi Mbunkah Daniel , Mamman Mohammed , Ndams Iliya Shehu , Nok Jonathan Andrew , Kelm Sørge , Weber Judith Sophie , Ngomtcho Sen Claudine Henriette , Shaida Stephen Saikiu , Chechet Gloria Dada , Gbem Thaddeus Terlumun , Nok Jonathan Andrew , Mamman Mohammed , Achukwi Mbunkah Daniel , Kelm Sørge , Adams Emily R , Malele Imna I , Msangi Atway R , Gibson Wendy C , Hamilton Patrick B , Stevens Jamie R , Gaunt Michael W , Gidley Jennifer , Gibson Wendy C , Ngomtcho Sen Claudine Henriette , Weber Judith Sophie , Ngo Bum Elisabeth , Gbem Thaddeus Terlumun , Kelm Sørge , Achukwi Mbunkah Daniel . Title: Trypanosomes are monophyletic: evidence from genes for glyceraldehyde phosphate dehydrogenase and small subunit ribosomal RNA Identifier: https://doi.org/10.1016/j.ijpara.2004.08.011 Type: DOI Relation: References Year: 2004 Source: International Journal for Parasitology Authors: Shaida Stephen Saikiu , Weber Judith Sophie , Gbem Thaddeus Terlumun , Ngomtcho Sen Claudine Henriette , Musa Usman Baba , Achukwi Mbunkah Daniel , Mamman Mohammed , Ndams Iliya Shehu , Nok Jonathan Andrew , Kelm Sørge , Weber Judith Sophie , Ngomtcho Sen Claudine Henriette , Shaida Stephen Saikiu , Chechet Gloria Dada , Gbem Thaddeus Terlumun , Nok Jonathan Andrew , Mamman Mohammed , Achukwi Mbunkah Daniel , Kelm Sørge , Adams Emily R , Malele Imna I , Msangi Atway R , Gibson Wendy C , Hamilton Patrick B , Stevens Jamie R , Gaunt Michael W , Gidley Jennifer , Gibson Wendy C , Ngomtcho Sen Claudine Henriette , Weber Judith Sophie , Ngo Bum Elisabeth , Gbem Thaddeus Terlumun , Kelm Sørge , Achukwi Mbunkah Daniel . Title: Molecular screening of tsetse flies and cattle reveal different Trypanosoma species including T. grayi and T. theileri in northern Cameroon Identifier: https://doi.org/10.1186/s13071-017-2540-7 Type: DOI Relation: References Year: 2017 Source: Parasites & Vectors Authors: Shaida Stephen Saikiu , Weber Judith Sophie , Gbem Thaddeus Terlumun , Ngomtcho Sen Claudine Henriette , Musa Usman Baba , Achukwi Mbunkah Daniel , Mamman Mohammed , Ndams Iliya Shehu , Nok Jonathan Andrew , Kelm Sørge , Weber Judith Sophie , Ngomtcho Sen Claudine Henriette , Shaida Stephen Saikiu , Chechet Gloria Dada , Gbem Thaddeus Terlumun , Nok Jonathan Andrew , Mamman Mohammed , Achukwi Mbunkah Daniel , Kelm Sørge , Adams Emily R , Malele Imna I , Msangi Atway R , Gibson Wendy C , Hamilton Patrick B , Stevens Jamie R , Gaunt Michael W , Gidley Jennifer , Gibson Wendy C , Ngomtcho Sen Claudine Henriette , Weber Judith Sophie , Ngo Bum Elisabeth , Gbem Thaddeus Terlumun , Kelm Sørge , Achukwi Mbunkah Daniel .