Molecular responses of calcifying and non-calcifying Antarctic benthic species to Ocean Acidification - Apoptotic activity

Southern Ocean organisms are thought to be particularly vulnerable to ocean acidification, as they inhabit cold waters where calcite-aragonite saturation states are naturally low. It is also generally assumed that calcifying animals would be more affected by ocean acidification than non-calcifying ones. In this context, we aimed to study the impacts of reduced pH on the ascidia Cnemidocarpa verrucosa sp. A. Here, we used gene expression profiling and enzymatic activity to study the responses of that Antarctic benthic species to ocean acidification. We sampled Cnemidocarpa verrucosa sp. A. by scuba diving at approximately 15 m depth at Carlini station, Potter Cove, King George Island, Antarctica. Superoxide dismutase (SOD) activity was measured in the ascidia, samples (approximately 70 mg of brachial basket) were homogenized in 20 mM Tris-HCl, 1 mM EDTA, pH 7.6, with a ratio 1:4 w/v. Homogenates were centrifuged at 14,000 x g for 3 min at 4°C and the supernatant was used to measure SOD activity at 20°C following Livingstone et al. (1992) protocol. Supernatant was mixed with the measurement buffer (43 mM K₂HPO₄, 43 mM KH₂PO₄, 0.1 mM EDTA, pH 7.68), 5 mM Xanthina (Sigma X-0626), 100 µM Citocromo-C (Sigma C-2037), 0.3 mU/µl XOD (Xanthin-Oxidasa, Sigma X-4875) in 2 M (NH₄)2SO₄. The measurement was made in a photometer at 20°C, 550 nm wavelength, for 3 minutes, every 10 seconds. For the calculations, the total protein content of the samples was measured using the method of Bradford (1976). Superoxide dismutase activity was expressed in activity in the extract (mU) / amount of protein (mg). All measurements were made in triplicate.

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Servetto, Natalia, Ruiz, Micaela Belen, Martinez, Mariano, Harms, Lars, de Aranzamendi, M C, Alurralde, Gastón, Giménez, D, Abele, Doris, Held, Christoph, Sahade, Ricardo José (2023). Dataset: Molecular responses of calcifying and non-calcifying Antarctic benthic species to Ocean Acidification - Apoptotic activity. https://doi.org/10.1594/PANGAEA.956183

DOI retrieved: 2023

Additional Info

Field Value
Imported on November 30, 2024
Last update November 30, 2024
License CC-BY-4.0
Source https://doi.org/10.1594/PANGAEA.956183
Author Servetto, Natalia
Given Name Natalia
Family Name Servetto
More Authors
Ruiz, Micaela Belen
Martinez, Mariano
Harms, Lars
de Aranzamendi, M C
Alurralde, Gastón
Giménez, D
Abele, Doris
Held, Christoph
Sahade, Ricardo José
Source Creation 2023
Publication Year 2023
Resource Type text/tab-separated-values - filename: ANT_PotterCove_2015_Caspase
Subject Areas
Name: BiologicalClassification

Name: Chemistry

Related Identifiers
Title: Molecular responses of calcifying and non-calcifying Antarctic benthic species to Ocean Acidification - Superoxide dismutase activity
Identifier: https://doi.org/10.1594/PANGAEA.956185
Type: DOI
Relation: References
Year: 2023
Authors: Servetto Natalia , Ruiz Micaela Belen , Martinez Mariano , Harms Lars , de Aranzamendi M C , Alurralde Gastón , Giménez D , Abele Doris , Held Christoph , Sahade Ricardo José , Bradford Marion M , Livingstone D R , Lips F , Garcia Martinez P , Pipe R K .

Title: A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding
Identifier: https://doi.org/10.1016/0003-2697(76)90527-3
Type: DOI
Relation: References
Year: 1976
Source: Analytical Biochemistry
Authors: Servetto Natalia , Ruiz Micaela Belen , Martinez Mariano , Harms Lars , de Aranzamendi M C , Alurralde Gastón , Giménez D , Abele Doris , Held Christoph , Sahade Ricardo José , Bradford Marion M , Livingstone D R , Lips F , Garcia Martinez P , Pipe R K .

Title: Antioxidant enzymes in the digestive gland of the common mussel Mytilus edulis
Identifier: https://doi.org/10.1007/BF00702471
Type: DOI
Relation: References
Year: 1992
Source: Marine Biology
Authors: Servetto Natalia , Ruiz Micaela Belen , Martinez Mariano , Harms Lars , de Aranzamendi M C , Alurralde Gastón , Giménez D , Abele Doris , Held Christoph , Sahade Ricardo José , Bradford Marion M , Livingstone D R , Lips F , Garcia Martinez P , Pipe R K .