Molecular responses of calcifying and non-calcifying Antarctic benthic species to Ocean Acidification - Superoxide dismutase activity

Southern Ocean organisms are thought to be particularly vulnerable to ocean acidification, as they inhabit cold waters where calcite-aragonite saturation states are naturally low. It is also generally assumed that calcifying animals would be more affected by ocean acidification than non-calcifying ones. In this context, we aimed to study the impacts of reduced pH on the ascidia Cnemidocarpa verrucosa sp. A. Here, we used gene expression profiling and enzymatic activity to study the responses of that Antarctic benthic species to ocean acidification. We sampled Cnemidocarpa verrucosa sp. A. by scuba diving at approximately 15 m depth at Carlini station, Potter Cove, King George Island, Antarctica. Caspases 3/7 activity as indicators of apoptosis intensity was measured using the Caspase-Glow 3/7 Assay kit (Promega, USA) following the manufacturer's instructions. Samples were homogenized (16-33 mg) in lysis buffer consisting in 25 mM HEPES, 5 mM MgCl₂·6H₂O, 1 mM EGTA, 1 μg/mL pepstatin, 1 μg/mL leupectin, and 1 μg/mL aprotinin at a ratio 1:100 (Rivera-Ingraham et al., 2013) using a Precellys homogenizer (2 cycles at 5,500 x g at 4°C for 20 s). Homogenates were centrifuged at 13,000 x g at 4°C for 15 min and the supernatant was used to measure luminescence using Tristar LB941 plate reader (Berthold Technologies GmbH & Co. KG, Bad Wildbad, Germany). The total protein content of the samples was measured using the method of Bradford (1976). Caspase/Apoptotic activity was expressed as relative light units (RLU) per μg of protein × 104.

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Servetto, Natalia, Ruiz, Micaela Belen, Martinez, Mariano, Harms, Lars, de Aranzamendi, M C, Alurralde, Gastón, Giménez, D, Abele, Doris, Held, Christoph, Sahade, Ricardo José (2023). Dataset: Molecular responses of calcifying and non-calcifying Antarctic benthic species to Ocean Acidification - Superoxide dismutase activity. https://doi.org/10.1594/PANGAEA.956185

DOI retrieved: 2023

Additional Info

Field Value
Imported on November 30, 2024
Last update November 30, 2024
License CC-BY-4.0
Source https://doi.org/10.1594/PANGAEA.956185
Author Servetto, Natalia
Given Name Natalia
Family Name Servetto
More Authors
Ruiz, Micaela Belen
Martinez, Mariano
Harms, Lars
de Aranzamendi, M C
Alurralde, Gastón
Giménez, D
Abele, Doris
Held, Christoph
Sahade, Ricardo José
Source Creation 2023
Publication Year 2023
Resource Type text/tab-separated-values - filename: ANT_PotterCove_2015_SOD
Subject Areas
Name: BiologicalClassification

Name: Chemistry

Related Identifiers
Title: Molecular responses of calcifying and non-calcifying Antarctic benthic species to Ocean Acidification - Apoptotic activity
Identifier: https://doi.org/10.1594/PANGAEA.956183
Type: DOI
Relation: References
Year: 2023
Authors: Servetto Natalia , Ruiz Micaela Belen , Martinez Mariano , Harms Lars , de Aranzamendi M C , Alurralde Gastón , Giménez D , Abele Doris , Held Christoph , Sahade Ricardo José , Bradford Marion M , Rivera-Ingraham Georgina A , Rocchetta Iara , Meyer Stefanie , Abele Doris .

Title: A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding
Identifier: https://doi.org/10.1016/0003-2697(76)90527-3
Type: DOI
Relation: References
Year: 1976
Source: Analytical Biochemistry
Authors: Servetto Natalia , Ruiz Micaela Belen , Martinez Mariano , Harms Lars , de Aranzamendi M C , Alurralde Gastón , Giménez D , Abele Doris , Held Christoph , Sahade Ricardo José , Bradford Marion M , Rivera-Ingraham Georgina A , Rocchetta Iara , Meyer Stefanie , Abele Doris .

Title: Oxygen radical formation in anoxic transgression and anoxia-reoxygenation: Foe or phantom? Experiments with a hypoxia tolerant bivalve
Identifier: https://doi.org/10.1016/j.marenvres.2013.09.007
Type: DOI
Relation: References
Year: 2013
Source: Marine Environmental Research
Authors: Servetto Natalia , Ruiz Micaela Belen , Martinez Mariano , Harms Lars , de Aranzamendi M C , Alurralde Gastón , Giménez D , Abele Doris , Held Christoph , Sahade Ricardo José , Bradford Marion M , Rivera-Ingraham Georgina A , Rocchetta Iara , Meyer Stefanie , Abele Doris .