Seawater carbonate chemistry and growth and carbon fixation of Antarctic cryptophyte

We conducted a multiple-stressor experiment to evaluate the response of the still poorly studied key Antarctic cryptophyte species Geminigera cryophila (CCMP 2564, isolated from the Southern Ocean and obtained from Matt Johnson's Laboratory of Protistan Ecology at the Woods Hole Oceanography Institute, United States) to warming in combination with ocean acidification and high irradiance. Based on the thermal growth response of G. cryophila, we grew the cryptophyte at suboptimal (2°C) and optimal (4°C) temperatures in combination with two light intensities (medium light: 100 μmol photons/m2/s and high light [HL]: 500 μmol photons/m2/s) under ambient (400 μatm pCO2) and high pCO2 (1000 μatm pCO2) conditions.