Quantification of intracellular 'free' calcium in isolated coral cells of Stylophora pistillata in laboratory experiment

Despite its prominent role as an intracellular messenger in all organisms, intracellular free calcium ([Ca2+]i) has not been quantified in corals or cnidarians in general. Ratiometric calcium dyes and cell imaging have been key methods in successful research on [Ca2+]i in mammlian and plat model systems, and could be applied to corals. Here, we developed a procedure to quantity [Ca2+]i in isolated cells from the model coral species Stylophora pistillata using Indo-1 and confocal microscopy mainatined in aquarium culture at the Centre Scientifique de Monaco, Monaco between 2020 and 2023. We quantified [Ca2+]i in coral cells with and without intracellular dinoflagellate symbionts, and verified our procedure on cultured mammalian cells. We then used our procedure to measure changes in [Ca2+]i in coral cells exposed to the classic inhibitor of [Ca2+], thapsigargin, and also used it to record elevations in [Ca2+]i in coral cells undergoing apoptosis. Our procedure paves the way for future studies into intracellular calcium in corals and other cnidarians.

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Venn, Alexander A, Techer, Nathalie, Segonds, Natacha, Tambutté, Eric, Tambutté, Sylvie (2024). Dataset: Quantification of intracellular 'free' calcium in isolated coral cells of Stylophora pistillata in laboratory experiment. https://doi.org/10.1594/PANGAEA.968788

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Field Value
Imported on November 29, 2024
Last update November 29, 2024
License CC-BY-4.0
Source https://doi.pangaea.de/10.1594/PANGAEA.968788
Author Venn, Alexander A
Given Name Alexander A
Family Name Venn
More Authors
Techer, Nathalie
Segonds, Natacha
Tambutté, Eric
Tambutté, Sylvie
Resource Type text/tab-separated-values - filename: LabExp_S_pistillata_Ca2+
Subject Areas
Name: BiologicalClassification

Name: Biosphere

Related Identifiers
Title: Quantification of cytosolic 'free' calcium in isolated coral cells with confocal microscopy
Identifier: https://doi.org/10.1242/jeb.247638
Type: DOI
Relation: References
Year: 2024
Source: Journal of Experimental Biology
Authors: Venn Alexander A , Techer Nathalie , Segonds Natacha , Tambutté Eric , Tambutté Sylvie .