Twinnetworkdataset032

Other: The data comprises bright-field time-lapse images of zebrafish embryos acquired in multiple batches within multi-well plates using an Acquifer Imaging Machine. Embryos from incrosses of Tg(sebox:EGFP) and incrosses of TE zebrafish strains were used for imaging. Small-molecule signaling pathway modulator LDN-193189 was added to well A2 with a final concentration of 3.5 µM and to well A3 with a final concentration of 4 µM. Small-molecule signaling pathway modulator SB-505124 was added to wells B1-B3 with final concentrations of 4 µM in well B1, 5 µM in well B2, and 6 µM in well B3. Well A1 did not receive small-molecule signaling pathway modulator treatment. Imaging started with embryos between 2-3 hpf and ended with embryos between 27-28 hpf. Images of embryos were acquired at 180 acquisition time points with a time interval of 501 s between each acquisition time point. Individual embryo segments were identified and extracted using a trained neural network for object detection. Within this experiment folder, data are organized by microscope position and embryo number.