Online measurement data of continuous aqueous two-phase flotation (atpf) for enzyme purification

TechnicalRemarks: - The .csv files contain experimental data obtained from ATPF experiments at laboratory scale. - The flotation tank used contains 700 ml of the aqueous two-phase system (ATPS). The phase forming components were polyethylene glycol 1000 and trisodium citrate dihydrate. - Phospholipase A2 was used as the model enzyme and the model suspension was mixed with the bottom phase. - The flotation tank was conceptually divided into three compartments, each compartment containing a gassing unit and an electrical conductivity probe. The electrical conductivity probes were inserted 1 cm below the phase interface. - An UV/Vis spectrometer with flow cell was implemented at the top phase outlet to obtain the top phase concentration in real-time. The bottom phase concentration was calculated via a mass balance equation.

  • The file names contain the process parameters in the following order: feed concentration_gas flow rate combination (Vd_1-Vd_2-Vd_3, see also below)_residence time_volume ratio (top to bottom phase)_experiment number.
  • The .csv files contain the following experimental data:
  • t: time in min
  • R_top, R_bot: top and bottom phase recirculation (R = 1 for full recirculation, R = 0 for no recirculation)
  • c_bot_3, c_top_3: enzyme concentration obtained from UV/Vis measurements in % (w/w).
  • kappa_bot_1, kappa_bot_2, kappa_bot_3: Electrical conductivity measurements in all three compartments in mS/cm.
  • Qd_bot, Qd_top: Volume flows of top and bottom phase in ml/min.
  • Vd_1, Vd_2, Vd_3: Gas volume flows of the three gassing units in ml/min.

Cite this as

Lohfink, Kim Carina, Kirsch, Michel, Nirschl, Hermann (2024). Dataset: Online measurement data of continuous aqueous two-phase flotation (atpf) for enzyme purification. https://doi.org/10.35097/pn5npep7vfjn92fe

DOI retrieved: 2024

Additional Info

Field Value
Imported on November 28, 2024
Last update November 28, 2024
License CC BY 4.0 Attribution
Source https://doi.org/10.35097/pn5npep7vfjn92fe
Author Lohfink, Kim Carina
Given Name Kim Carina
Family Name Lohfink
More Authors
Kirsch, Michel
Nirschl, Hermann
Source Creation 2024
Publishers
Karlsruhe Institute of Technology
Production Year 2024
Publication Year 2024
Subject Areas
Name: Engineering

Related Identifiers
Identifier: https://publikationen.bibliothek.kit.edu/1000174648
Type: URL
Relation: IsIdenticalTo